A5035833387- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Genetics and Plant Breeding
- Plant Micronutrient Interactions and Effects
- Plant Genetic and Mutation Studies
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Crop Yield and Soil Fertility
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Genomics and Chromatin Dynamics
- Bioenergy crop production and management
- Polysaccharides and Plant Cell Walls
- RNA Research and Splicing
- Wheat and Barley Genetics and Pathology
- Metabolomics and Mass Spectrometry Studies
- Light effects on plants
Purdue University West Lafayette
2016-2025
University of Kansas
2024
University of Florida
2024
Research Institute of Molecular Pathology
2024
University of Oxford
2023
John Wiley & Sons (United Kingdom)
2023
Hudson Institute
2023
John Wiley & Sons (United States)
2023
South University
2016-2020
Plant (United States)
2017-2019
dwarf4 (dwf4) mutants of Arabidopsis display a dwarfed phenotype due to lack cell elongation. Dwarfism could be rescued by the application brassinolide, suggesting that DWF4 plays role in brassinosteroid (BR) biosynthesis. The locus is defined four mutant alleles. One these result T-DNA insertion. Plant DNA flanking insertion site was cloned and used as probe isolate entire gene. Sequence analysis revealed encodes cytochrome P450 monooxygenase with 43% identity putative steroid hydroxylating...
Genome duplication (or polyploidization) has occurred throughout plant evolutionary history and is thought to have driven the adaptive radiation of plants. We found that cytotype root, not genotype, determined majority heritable natural variation in leaf potassium (K) concentration Arabidopsis thaliana. Autopolyploidy also provided resistance salinity may represent an outcome enhanced K accumulation plants with higher ploidy.
The origins of maize were the topic vigorous debate for nearly a century, but neither current genetic model nor earlier archaeological models account totality available data, and recent work has highlighted potential contribution wild relative, Zea mays ssp. mexicana . Our population analysis reveals that origin modern can be traced to an admixture between ancient in highlands Mexico some 4000 years after domestication began. We show variation is key component diversity, both at individual...
Endoreduplication is a form of nuclear polyploidization that results in multiple, uniform copies chromosomes. This process common plants and animals, especially tissues with high metabolic activity, it generally occurs cells are terminally differentiated. In plants, endoreduplication well documented the endosperm cotyledons developing seeds, but also many throughout plant. It thought provides mechanism to increase level gene expression, function this has not been thoroughly investigated....
Abstract Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2),constitutive photomorphogenesis dwarfism(cpd), brassinosteroid insensitive1 (bri1), dwf4, have been reported to be defective in either biosynthesis or perception brassinosteroids. We present further ofdwf1-1 additional dwf1 alleles. Feeding tests with brassinosteroid-biosynthetic intermediates revealed that can rescued...
Abstract Maize starchy endosperm mutants have kernel phenotypes that include a brittle texture, susceptibility to insect pests, and inferior functional characteristics of products made from their flour. At least 18 such been identified, but only in the cases opaque2 (o2) floury2 (fl2), which affect different aspects storage protein synthesis, is molecular basis mutation known. To better understand relationship between these biochemical bases, we characterized amino acid composition, as well...
Genome duplication, which results in polyploidy, is disruptive to fundamental biological processes. duplications occur spontaneously a range of taxa and problems such as sterility, aneuploidy, gene expression aberrations are common newly formed polyploids. In mammals, genome duplication associated with cancer spontaneous abortion embryos. Nevertheless, stable polyploid species both plants animals. Understanding how natural selection enabled these overcome early challenges can provide...
Genome-wide association studies (GWAS) have identified loci linked to hundreds of traits in many different species. Yet, because linkage equilibrium implicates a broad region surrounding each locus, the causal genes often remain unknown. This problem is especially pronounced nonhuman, nonmodel species, where functional annotations are sparse and there frequently little information available for prioritizing candidate genes. We developed computational approach, Camoco, that integrates by GWAS...
Most mineral elements found in plant tissues come exclusively from the soil, necessitating that plants adapt to highly variable soil compositions survive and thrive. Profiling element concentrations genetically diverse populations is providing insights into plant-environment interactions control elemental accumulation, as well identifying underlying genes. The resulting molecular understanding of adaptation environment both demonstrates how soils can shape genetic diversity provides...
A small number of phytohormones dictate the pattern plant form affecting fitness via reproductive architecture and plant's ability to forage for light, water, nutrients. Individual phytohormone contributions have been studied extensively, often following a single component architecture, such as height or branching. Both brassinosteroid (BR) gibberellin (GA) affect height, branching, sexual organ development in maize (Zea mays). We identified molecular basis nana plant2 (na2) phenotype...
Transposons are generally kept silent by epigenetic mechanisms including DNA methylation. Here, we identified a pair of H arbinger transposon-derived proteins (HDPs), HDP1 and HDP2, as anti-silencing factors in Arabidopsis. hdp1 hdp2 mutants displayed an enhanced silencing transgenes some transposons. Phylogenetic analyses revealed that HDP2 were co-domesticated from the Harbinger transposon-encoded transposase DNA-binding protein, respectively. interacts with nucleus, analogous to their...
Identifying the genetic basis of local adaptation and fitness trade-offs across environments is a central goal evolutionary biology. Cold acclimation an adaptive plastic response for surviving seasonal freezing, costs may be general mechanism in temperate zone species. Starting with locally adapted ecotypes
Abstract Polyploidy, the inheritance of more than two genome copies per cell, has played a major role in evolution higher plants. Little is known about transition from diploidy to polyploidy but some species, triploids are thought function as intermediates this transition. In contrast, other species triploidy viewed block. We investigated responses Arabidopsis thaliana triploidy. The genetic variability was tested by comparing generated crosses between Col-0, diploid, and either natural...
We report the characterization of a maize Wee1 homologue and its expression in developing endosperm. Using 0.8-kb cDNA from an expressed sequence tag project, we isolated 1.6-kb (ZmWee1), which encodes protein 403 aa with calculated molecular size 45.6 kDa. The deduced amino acid shows 50% identity to kinase domain human Wee1. Overexpression ZmWee1 Schizosaccharomyces pombe inhibited cell division caused cells enlarge significantly. Recombinant obtained Escherichia coli is able inhibit...
The molecular mechanisms underlying lethality of F1 hybrids between diverged parents are one target speciation research. Crosses diploid and tetraploid individuals the same genotype can result in lethality, this dosage-sensitive incompatibility plays a role polyploid speciation. We have identified variation interploidy crosses Arabidopsis thaliana determined genetic architecture maternally expressed via QTL mapping. A single large-effect QTL, DR. STRANGELOVE 1 (DSL1), was as well two with...
Aneuploid cells are characterized by incomplete chromosome sets. The resulting imbalance in gene dosage has phenotypic consequences that specific to each karyotype. Even the case of Down syndrome, most viable and studied form human aneuploidy, mechanisms underlying connected phenotypes remain mostly unclear. Because their tolerance plants provide a powerful system for genome-wide investigation aneuploid syndromes, an approach is not feasible animal systems. Indeed, many plant species,...
Allotetraploids carry pairs of diverged homoeologs for most genes. With the genome doubled in size, number putative interactions is enormous. This poses challenges on how to coordinate two disparate genomes, and creates opportunities by enhancing phenotypic variation. New combinations alleles co-adapt respond new environmental pressures. Three stages allopolyploidization process - parental species divergence, hybridization, duplication have been well analyzed. The last stage evolutionary...
Whole-genome duplication resulting from polyploidy is ubiquitous in the evolutionary history of plant species. Yet, polyploids must overcome meiotic challenge pairing, recombining, and segregating more than two sets chromosomes. Using genomic sequencing synthetic natural allopolyploids Arabidopsis thaliana arenosa, we determined that dosage variation chromosomal translocations consistent with homoeologous pairing were frequent allopolyploids. To test role structural differentiation versus...
Sulphur (S) is an essential element for all living organisms. The uptake, assimilation and metabolism of S in plants are well studied. However, the regulation homeostasis remains largely unknown. Here, we report on identification characterisation more sulphur accumulation1 (msa1-1) mutant. MSA1 protein localized to nucleus required both S-adenosylmethionine (SAM) production DNA methylation. Loss function nuclear localised leads a reduction SAM roots strong S-deficiency response even at ample...
A major challenge in global crop production is mitigating yield loss due to plant diseases. One of the best strategies control these losses through breeding for disease resistance. barrier identification resistance genes quantification severity, which typically based on determination a subjective score by human observer. We hypothesized that image-based, non-destructive measurements morphology over an extended period after pathogen infection would capture subtle quantitative differences...
Two maize (Zea mays) cyclin-dependent kinase (CDK) inhibitors, Zeama;KRP;1 and Zeama;KRP;2, were characterized shown to be expressed in developing endosperm. Similar the CDK inhibitors Arabidopsis (Arabidopsis thaliana) tobacco (Nicotiana tabacum), proteins contain a carboxy-terminal region related inhibitory domain of mammalian Cip/Kip inhibitors. is present endosperm between 7 21 d after pollination, period that encompasses onset endoreduplication, while Zeama;KRP;2 protein declines during...
The genome of the Mastreviruses encodes a replication-associated protein (RepA) that interacts with members plant retinoblastoma-related family, which are putative cell cycle regulators. Expression ZmRb1, maize gene, and RepA inhibited stimulated, respectively, division in tobacco cultures. effect was mitigated by over-expression ZmRb1. increased transformation frequency callus growth rate high type II germplasm. RepA-containing transgenic calli remained embryogenic, were readily...