A5049616628- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Plant Virus Research Studies
Corteva (United States)
2018-2020
Pioneer Hi-Bred
1995-2018
Experimental Station
2002
University of Arizona
2002
While transformation of the major monocot crops is currently possible, process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond reach most academic laboratories. Here, we report a approach involving overexpression maize (Zea mays) Baby boom (Bbm) Wuschel2 (Wus2) genes, which produced high frequencies in numerous previously nontransformable inbred lines. For example, Pioneer PHH5G recalcitrant...
Constitutive expression of the
Abstract The use of Baby boom ( Bbm ) and Wuschel2 Wus2 has made maize transformation more efficient across an increasingly wide range inbreds. However, the benefits have come with requirement excising these helper components to enable plant regeneration, which adds size T-DNA, complexity system. A new system advantages smaller simplicity for selectable marker gene-containing T-DNA is described. First, expression Zm-Wus2 alone driven by Pltp promoter Zm - pro ), was determined be sufficient...
The genome of the Mastreviruses encodes a replication-associated protein (RepA) that interacts with members plant retinoblastoma-related family, which are putative cell cycle regulators. Expression ZmRb1, maize gene, and RepA inhibited stimulated, respectively, division in tobacco cultures. effect was mitigated by over-expression ZmRb1. increased transformation frequency callus growth rate high type II germplasm. RepA-containing transgenic calli remained embryogenic, were readily...
Retinoblastoma-related (RBR) genes inhibit the cell cycle primarily by repressing adenovirus E2 promoter binding factor (E2F) transcription factors, which drive expression of numerous required for DNA synthesis and progression. The RBR-E2F pathway is conserved in plants, but cereals such as maize are characterized having a complex RBR gene family with at least 2 functionally distinct members, RBR1 RBR3. Although has clear inhibitory function, it not known whether RBR3 positive or negative...
Use of the morphogenic genes Baby Boom (Bbm) and Wuschel2 (Wus2), along with new ternary constructs, has increased genotype range type explants that can be used for maize transformation. In addition, altering ectopic expression pattern Bbm/Wus2 resulted in rapid transformation methods are faster applicable to a broader inbreds. However, compromise quality regenerated plants, leading sterility. We reasoned excising after but before regeneration would increase production fertile T0 plants....
Endosperm development in maize (Zea mays L.) and related cereals comprises a cell proliferation stage followed by period of rapid growth coupled to endoreduplication. Regulation the cycle developing endosperm is poorly understood. We have characterized various subunits cyclin-dependent kinase (CDK) complexes, master regulators all eukaryotes. A-, B-, D-type cyclins as well A- B-type kinases were with respect their RNA protein expression profiles. Two main patterns identified: one showing...
ABSTRACT Use of the morphogenic genes Baby Boom ( Bbm ) and Wuschel2 Wus2 ), along with new ternary constructs, has increased genotype range type explants that can be used for maize transformation. In addition, altering ectopic expression pattern Bbm/Wus2 resulted in rapid transformation methods are faster applicable to a broader inbreds. However, compromise quality regenerated plants, leading sterility. We reasoned excising after but before regeneration would increase production fertile T0...