A5100436536- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Algal biology and biofuel production
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Venous Thromboembolism Diagnosis and Management
- Polyamine Metabolism and Applications
- Protease and Inhibitor Mechanisms
- CAR-T cell therapy research
- Blood Coagulation and Thrombosis Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Enzyme Production and Characterization
- Bioactive Compounds and Antitumor Agents
- Plant Genetic and Mutation Studies
- Weed Control and Herbicide Applications
- Natural product bioactivities and synthesis
- Antioxidant Activity and Oxidative Stress
- RNA regulation and disease
- Phytochemical Studies and Bioactivities
- Plant Reproductive Biology
Nanjing Agricultural University
2023
Corteva (United States)
2019-2020
Shanghai Academy of Agricultural Sciences
2019
Xiamen University
2018
Pioneer Hi-Bred
2015-2016
Dalian Medical University
2013
Chinese Academy of Sciences
2008
Institute of Botany
2008
While transformation of the major monocot crops is currently possible, process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond reach most academic laboratories. Here, we report a approach involving overexpression maize (Zea mays) Baby boom (Bbm) Wuschel2 (Wus2) genes, which produced high frequencies in numerous previously nontransformable inbred lines. For example, Pioneer PHH5G recalcitrant...
We have developed a novel hybridization platform that utilizes nuclear male sterility to produce hybrids in maize and other cross-pollinating crops. A key component of this is process termed Seed Production Technology (SPT). This incorporates transgenic SPT maintainer line capable propagating nontransgenic male-sterile lines for use as female parents hybrid production. The homozygous recessive sterile transformed with construct containing (i) complementary wild-type fertility gene restore...
Abstract CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture productivity, but the possibility of unintended off-target edits evoked some concerns. Here we employ a three-step strategy to investigate Cas9 nuclease specificity in complex plant genome. Our approach pairs computational prediction with genome-wide biochemical detection followed by validation maize plants. results reveal high frequency (up 90%) on-target editing no evidence cleavage activity when...
Modern maize hybrids often contain biotech and native traits. To-date all traits have been randomly inserted in the genome. Consequently, developing with multiple is expensive, time-consuming complex. Here we report using CRISPR-Cas9 to generate a Complex Trait Locus (CTL) facilitate trait stacking. A CTL consists of preselected sites positioned within small well-characterized chromosomal region where genes are inserted. We generated individual lines, each carrying site-specific insertion...
The purpose of this study was to investigate the ameliorating effect dioscin (1) on multidrug resistance (MDR) in adriamycin (ADR)-resistant erythroleukemic cells (K562/adriamycin, K562/ADR) and clarify molecular mechanisms involved. High levels 1 (MDR1) mRNA protein reduced ADR retention were found K562/ADR compared with parental (K562). Dioscin (1), a constituent plants genus Discorea, significantly inhibited MDR1 expression promoter nuclear factor κ-B (NF-κB) activity cells. suppression...
Protein glutaminase (PG) is a novel protein modification biotechnology that increasingly being used in the food industry. However, current level of fermentation PG-producing strains still does not meet requirements industrial production. To obtain mutant with high PG production, atmospheric and room temperature plasma (ARTP) combined LiCl chemical mutagen were mutagenesis producing Chryseobacterium proteolyticum 1003.A strain (WG15) was successfully obtained based on malonic acid resistance...
Effective delivery of therapeutic genes or small molecular drugs into macrophages is important for cell based immune therapy, but it remains a challenge due to the intracellular reactive oxygen species and endosomal degradation therapeutics inside cells. In this report, star-like amphiphilic biocompatible β-cyclodextrin-graft-(poly(ε-caprolactone)-block-poly(2-(dimethylamino) ethyl methacrylate)x (β-CD-g-(PCL-b-PDMAEMA)x ) copolymer, consisting cyclodextrin core, hydrophobic...
Expression levels and clinical significance of matrix metalloproteinase-1 (MMP1), MMP-2 inflammatory factors in the serum patients with deep venous thrombosis (DVT) lower extremity were investigated. Fifty untreated DVT selected as group, 50 undergoing health examination enrolled normal control group. Enzyme-linked immunosorbent assay (ELISA) was used to determine MMP-1, MMP-2, interleukin-6 (IL-6), IL-8 tumor necrosis factor-α (TNF-α) serum. Western blotting adopted detect expression MMP-1...