A5101769595- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Climate variability and models
- Electrospun Nanofibers in Biomedical Applications
- Connective tissue disorders research
- Aortic Disease and Treatment Approaches
- Venous Thromboembolism Diagnosis and Management
- Tropical and Extratropical Cyclones Research
- PI3K/AKT/mTOR signaling in cancer
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Tissue Engineering and Regenerative Medicine
- High voltage insulation and dielectric phenomena
- Protein Tyrosine Phosphatases
- Congenital heart defects research
- Cancer Genomics and Diagnostics
- Meteorological Phenomena and Simulations
- Reproductive Biology and Fertility
- Coronary Interventions and Diagnostics
- Breast Cancer Treatment Studies
- Fire effects on ecosystems
- Advanced ceramic materials synthesis
- Fibroblast Growth Factor Research
Northeast Institute of Geography and Agroecology
2024-2025
Chinese Academy of Sciences
2016-2025
Chongqing Cancer Hospital
2014-2025
Chongqing University
2024-2025
Peking University Shenzhen Hospital
2020-2025
Northeast Forestry University
2025
Shanxi Agricultural University
2024
Shenzhen Institutes of Advanced Technology
2016-2024
Shandong Provincial QianFoShan Hospital
2004-2024
Beijing Tsinghua Chang Gung Hospital
2024
The molecular mechanisms responsible for the development and progression of atherosclerotic lesions have not been fully established. Here, we investigated role played by endothelial-to-mesenchymal transition (EndMT) its key regulator FGF receptor 1 (FGFR1) in atherosclerosis. In cultured human endothelial cells, both inflammatory cytokines oscillatory shear stress reduced FGFR1 expression activated TGF-β signaling. We further explored link between disrupted signaling atherosclerosis...
Atherosclerosis is driven by synergistic interactions between pathological, biomechanical, inflammatory, and lipid metabolic factors. Our previous studies demonstrated that absence of caveolin-1 (Cav1)/caveolae in hyperlipidemic mice strongly inhibits atherosclerosis, which was attributed to activation endothelial nitric oxide (NO) synthase (eNOS) increased production NO reduced inflammation low-density lipoprotein trafficking. However, the contribution eNOS athero-protection Cav1 exact...
MicroRNA-21 was upexpressed in gastric cancer (GC) indicating that it is a potential diagnostic biomarker for GC. In this study, 50 GC patients and healthy controls were recruited. miR-21 levels serum peripheral blood mononuclear cells (PBMCs) quantified using quantitative real-time PCR. CA199, CEA measured electrochemiluminescence assay. The sensitivity specificity of circulating miR-21, CA199 diagnosis, the correlation to clinicopathological features, value different stages determined....
Human endothelial cells are initiators and targets of the rejection response. Pre-operative modification by small interfering RNA transfection could shape nature host response post-transplantation. Ablation cell class II major histocompatibility complex molecules targeting transactivator can reduce capacity human to recruit activate alloreactive T cells. Here, we report development RNA-releasing poly(amine-co-ester) nanoparticles, distinguished their high content a hydrophobic lactone. We...
Summary Caseinolytic peptidase P mediates degradation of unfolded mitochondrial proteins and activates protein response (mt UPR ) to maintain homeostasis. Clpp −/− female mice generate a lower number mature oocytes two‐cell embryos, no blastocysts. have smaller mitochondria, with aspect ratio (length/width), decreased expression genes that promote fusion. A 4‐fold increase in atretic follicles at 3 months, reduced primordial 6–12 months are observed ovaries. This is associated upregulation...
Smooth muscle cell (SMC) proliferation has been thought to limit the progression of thoracic aortic aneurysm and dissection (TAAD) because loss medial cells associates with advanced disease. We investigated effects SMC in media by conditional disruption Tsc1, which hyperactivates mTOR complex 1. Consequent hyperplasia led progressive degeneration TAAD. In addition diminished contractile synthetic functions, fate-mapped SMCs displayed increased proteolysis, endocytosis, phagocytosis,...
Climate change and human activity are increasing the frequency of wildfires in peatlands threatening permafrost peatland carbon pools. In Northeast China, low-severity prescribed fires conducted annually on to reduce risk wildfires. These typically do not burn surface peat but lead loss vegetation introduction pyrogenic carbon. However, long-term effects repeated soil stability these ecosystems remain unclear. Thus, we fire experiments over 3 years Great Khingan Mountains. Our findings...
Abstract Smooth muscle cells (SMCs) in normal blood vessels exist a highly differentiate state characterized by expression of SMC-specific contractile proteins (“contractile phenotype”). Following vessel injury vivo or when cultured vitro the presence multiple growth factors, SMC undergo phenotype switch loss markers and appearance non-muscle (“proliferative While number factors have been reported to modulate this process, its regulation remains uncertain. Here we show that induction FGF...
Transforming growth factor-beta is a pleiotropic cytokine having diverse roles in vascular morphogenesis, homeostasis, and pathogenesis. Altered activity of signaling through transforming has been implicated thoracic aortic aneurysms dissections, conditions characterized by reduced structural integrity the wall that associates with altered biomechanics mechanobiology. We quantify contrast passive active biaxial biomechanical properties ascending proximal descending aorta mouse model...
Research Article13 May 2016Open Access Source DataTransparent process Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression Pei-Yu Chen Corresponding Author Department of Internal Medicine, Yale Cardiovascular Center, University School New Haven, CT, USA Search for more papers by this author Lingfeng Qin Surgery, Guangxin Li Vascular The First Hospital China Medical University, Shenyang, George Tellides Michael Simons orcid.org/0000-0003-0348-7734 Cell Biology, Information...
Rationale: Complement activation contributes to multiple immune-mediated pathologies. In late allograft failure, donor-specific antibody deposits complement membrane attack complexes (MAC) on graft endothelial cells (ECs), substantially increasing their immunogenicity without causing lysis. Internalized MAC stabilize NIK (NF-κB [nuclear factor kappa-light-chain-enhancer of activated B cells]–inducing kinase) protein Rab5+MAC+ endosomes, activating noncanonical NF-κB signaling. However, the...
Abstract Novel biological vascular conduits, such as decellularized tissue engineered grafts (TEVGs) are hindered by high thrombogenicity. To mimic the antithrombogenic surface of native vessels with a continuous glycosaminoglycan layer that is present on endothelial cells (ECs), hyaluronic acid (HA) modified established, to effectively shield blood platelets from collagen‐triggered activation. Using amine groups 4 mm diameter TEVGs, HA hydrogel coating built via bifunctional thiol‐reactive...
Biglycan, a member of the small leucine-rich proteoglycan family, has been implicated in development and progression human cancers. However, clinical significance biglycan expression gastric cancer not determined. In present study, mRNA protein concentrations were analyzed using quantitative realtime reverse transcription polymerase chain reaction Western blot 69 adjacent non-tumorous tissues, respectively. Biglycan was further assessed immunohistochemistry tissue microarrays that contained...
Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21inhibitor significantly increased levels BGC-823, but exerted an opposite effect. transfected had less cellular toxicity host cells compared conventional transfection methods. The promoted...
Significance Complement activation contributes to host defense and immunopathology. We recently discovered that membrane attack complexes (MAC), the terminal effector mechanisms of complement, activate proinflammatory functions in human endothelial cells (ECs) via noncanonical NF-ΚB signaling. Here we elucidate initial steps how MACs this pathway. formed on surface ECs are rapidly internalized clathrin-mediated endocytosis into Rab5 + endosomes, which subsequently recruit activated Akt a...
Mechanical homeostasis promotes proper aortic structure and function. Pathological conditions may arise, in part, from compromised or lost homeostasis. There is thus a need to quantify the homeostatic state when it emerges. Here we changes mechanical loading, geometry, structure, function of murine aorta late prenatal period into maturity.
Abstract Salicylic acid (SA) is a phytohormone required for plant growth and defense signaling. There are two major SA biosynthesis pathways in plants: the isochorismate synthase (ICS) pathway phenylalanine ammonia-lyase (PAL) pathway. It has been demonstrated several species, including model Arabidopsis , that derived predominantly from ICS Here, we employed CRISPR/Cas9 system to generate knockout mutants rice ( Oryza sativa L.). The Osics display severe defects, completely devoid of...
Context: The sensitivity and specificity of blood microRNA-421 (miR-421) as a gastric cancer (GC) biomarker has not been determined.Objective: To investigate the diagnostic value miR-421 GC biomarker.Materials methods: in serum peripheral mononuclear cells (PBMCs) 90 patients controls was detected by quantitative real-time PCR. correlation to clinicopathological features well comparing carcinoembryonic antigen (CEA) 125 (CA-125) were analyzed.Results: increased significantly than controls....