A5089194050- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- Circular RNAs in diseases
- Child Nutrition and Water Access
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Food Security and Health in Diverse Populations
- Poverty, Education, and Child Welfare
- Cancer, Lipids, and Metabolism
- Urinary Tract Infections Management
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Child Nutrition and Feeding Issues
- Health and Well-being Studies
- Phagocytosis and Immune Regulation
- Pluripotent Stem Cells Research
- Healthcare Education and Workforce Issues
- Advanced Glycation End Products research
- TGF-β signaling in diseases
- PI3K/AKT/mTOR signaling in cancer
- Cholesterol and Lipid Metabolism
- Problem Solving Skills Development
Yale University
2014-2024
Civil Service Hospital
2021-2023
Ministry of Health and Population
2023
Grande International Hospital
2022
Kathmandu Medical College Teaching Hospital
2022
B.P. Koirala Institute of Health Sciences
2022
Center for Vascular Biology Research
2018-2021
Karnali Academy of Health Sciences
2018
Kagoshima Medical Center
2018
National Hospital Organization
2018
To study the efficacy of anti-miRNA-33 therapy on progression atherosclerosis.Ldlr(-/-) mice were injected subcutaneously with PBS, control, or anti-miR-33 oligonucleotides weekly and fed a Western diet for 12 weeks. At end treatment, expression miR-33 target genes was increased in liver aorta, demonstrating effective inhibition function. Interestingly, plasma high-density lipoprotein (HDL)-cholesterol significantly anti-miR-33-treated but only when they chow diet. However, HDL isolated from...
Alterations in ectopic lipid deposition and circulating lipids are major risk factors for developing cardiometabolic diseases. Angiopoietin-like protein 4 (ANGPTL4), a that inhibits lipoprotein lipase (LPL), controls fatty acid (FA) uptake adipose oxidative tissues regulates triacylglycerol-rich (TAG-rich) lipoproteins. Unfortunately, global depletion of ANGPTL4 results severe metabolic abnormalities, inflammation, fibrosis when mice fed high-fat diet (HFD), limiting our understanding the...
Brown adipose tissue (BAT) controls triglyceride-rich lipoprotein (TRL) catabolism. This process is mediated by the lipase (LPL), an enzyme that catalyzed hydrolysis of triglyceride (TAG) in glycerol and fatty acids (FA), which are burned to generate heat. LPL activity regulated angiopoietin-like 4 (ANGPTL4), a secretory protein produced tissues (AT), liver, kidney, muscle. While role ANGPTL4 regulating metabolism well established, specific contribution BAT derived controlling lipid glucose...
Summary The miR-143/145 cluster regulates VSMC specific gene expression, thus controlling differentiation, plasticity and contractile function, promoting the phenotypic switch from a contractile/non-proliferative to migrating/proliferative state. More recently increased miR-145 expression was observed in human carotid atherosclerotic plaques symptomatic patients. goal of this study investigate contribution during atherogenesis by generating mice lacking on an Ldlr-deficient background....
Significance Previous work has shown that foamy macrophages accumulate desmosterol, a cholesterol biosynthetic intermediate. However, the participation of desmosterol in regulating functions cholesterol-laden physiological context atherosclerosis remained elusive. In this work, we selectively deplete by overexpressing DHCR24, enzyme catalyzes conversion into cholesterol, myeloid cells and demonstrate content atherosclerotic plaque controls their function activation. Molecular analysis...
Hepatic uptake and biosynthesis of fatty acids (FAs), as well the partitioning FAs into oxidative, storage, secretory pathways, are tightly regulated processes. Dysregulation one or more these processes can promote excess hepatic lipid accumulation, ultimately leading to systemic metabolic dysfunction. Angiopoietin-like-4 (ANGPTL4) is a protein that inhibits lipoprotein lipase (LPL) modulates triacylglycerol (TAG) homeostasis. To understand role ANGPTL4 in liver metabolism under normal...
Abstract AgRP neurons drive hunger, and excessive nutrient intake is the primary driver of obesity associated metabolic disorders. While many factors impacting central regulation feeding behavior have been established, role microRNAs in this process poorly understood. Utilizing unique mouse models, we demonstrate that miR-33 plays a critical neurons, loss leads to increased feeding, obesity, dysfunction mice. These effects include multiple target genes involved mitochondrial biogenesis fatty...
Abnormal remodeling of atherosclerotic plaques can lead to rupture, acute myocardial infarction, and death. Enhancement plaque extracellular matrix (ECM) may improve morphology stabilize lesions. Here, we demonstrate that chronic administration LNA-miR-29 into an mouse model improves indices morphology. This occurs due upregulation miR-29 target genes the ECM (col1A col3A) resulting in reduced lesion size, enhanced fibrous cap thickness, necrotic zones. Sustained treatment did not affect...
Abstract Background Despite consistent efforts to enhance child nutrition, poor nutritional status of children continues be a major public health problem in Nepal. This study identified the predictors severe acute malnutrition (SAM) among aged 6 59 months two districts Methods We used data from cross-sectional conducted admitted Outpatient Therapeutic Care Centers (OTCC). The was assessed using mid-upper arm circumference (MUAC) measurement. To determine which variables predict occurrence...
miR-33 is an intronic microRNA within the gene encoding SREBP2 transcription factor. Like its host gene, has been shown to be important regulator of lipid metabolism. Inhibition promote cholesterol efflux in macrophages by targeting transporter ABCA1, thus reducing atherosclerotic plaque burden. also improve high-density lipoprotein (HDL) biogenesis liver and increase circulating HDL-C levels both rodents nonhuman primates. However, evaluating extent which these changes HDL metabolism...
Angiopoietin-like 4 (ANGPTL4), a key protein involved in lipoprotein metabolism, has diverse effects. There is an association between Angptl4 and diabetic kidney disease; however, this not been well investigated. We show that both podocyte- tubule-specific ANGPTL4 are crucial fibrogenic molecules diabetes. Diabetes accelerates the phenotype control mice but mutant mice. The protective effect observed correlated with reduction stimulator of interferon genes pathway activation, expression...
The present work highlights astrocytic fatty acid homeostasis as a critical regulator of susceptibility to develop obesity.
Atherosclerosis is the major cause of death and disability in diabetic obese subjects with insulin resistance. Akt2, a phosphoinositide-dependent serine-threonine protein kinase, highly express insulin-responsive tissues; however, its role during progression atherosclerosis remains unknown. Thus, we aimed to investigate contribution Akt2 atherosclerosis. We found that germ-line Akt2-deficient mice develop similar atherosclerotic plaques as wild-type despite higher plasma lipids glucose...
The role of cell-specific ANGPTL4 is not well known in the context ECs, specifically pathological angiogenesis and its relation to diabetic kidney disease. Here, we demonstrate that endothelial required induce a metabolic phenotype favors mesenchymal activation ECs tubules conditions. Diabetes accelerates fibrogenesis control mice however, same effects were observed endothelial-cell specific knock out mice. This diabetes directly linked with neovascularization, leakage, lipid glycolysis...
Epigenetic modifications of the genome, including DNA methylation, histone methylation/acetylation, and noncoding RNAs, have been reported to play a fundamental role in regulating immune response during progression atherosclerosis. SETDB2 is member KMT1 family lysine methyltransferases, members this typically methylate H3 Lys9 (H3K9), an epigenetic mark associated with gene silencing. Previous studies shown that involved innate adaptive immunity, proinflammatory response, hepatic lipid...
Non-communicable Diseases are an alarming public health emergency in Nepal. Owing to the risk of NCD's Nepal, Government Nepal has developed a Multisectoral Action Plan for Disease 2014-2020 and adopted World Health Organization Package Essential protocol. Prior its implementation baseline study been carried out assess status facilities Nepal.A descriptive cross-sectional was Kailali Ilam district encompassing total 92 facilities. A set structured questionnaire interview guideline used...
In this study, we used a polydimethylsiloxane (PDMS)-based platform for the generation of intact, perfusion-competent microvascular networks in vitro. COMSOL Multiphysics, finite-element analysis and simulation software package, was to obtain simulated velocity, pressure, shear stress profiles. Transgene-free human induced pluripotent stem cells (hiPSCs) were differentiated into partially arterialized endothelial (hiPSC-ECs) 5 d under completely chemically defined conditions, using small...
Background: Type II Diabetes Mellitus (Type DM) is a metabolic disorder characterized by glucotoxicity and lipotoxicity. Marker of glycemic control are HbA1c, fasting blood glucose postprandial glucose. There altered lipid parameters in DM which possess significant cardiovascular risk to the patient. Recent studies have shown that ceruloplasmin, an inflammatory marker having antioxidant property,is ideal know status, degree insulin resistance cancer risk.Aims Objectives: Present study was...
Background: Medical undergraduates are more prone to emotional distress in comparison the general population and non-medical undergraduates. This study aimed identify prevalence factors associated with depression anxiety symptoms among undergraduate medical students. Methods: A cross-sectional was conducted 204 students a institute Kathmandu. Depression, Anxiety Stress Scale-42 used of symptoms. The data were analyzed using multivariable logistic regression models.Results: 30.9% 38.7%...