A5018645804- Tissue Engineering and Regenerative Medicine
- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Electrospun Nanofibers in Biomedical Applications
- Cardiovascular Function and Risk Factors
- Connective tissue disorders research
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Cardiac Imaging and Diagnostics
- RNA Interference and Gene Delivery
- Hydrogels: synthesis, properties, applications
- Elasticity and Material Modeling
- Optical Imaging and Spectroscopy Techniques
- Inflammatory Bowel Disease
- Nanoparticle-Based Drug Delivery
- Aortic Disease and Treatment Approaches
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cardiovascular Health and Disease Prevention
- Additive Manufacturing and 3D Printing Technologies
- Diversity and Career in Medicine
- Nanoplatforms for cancer theranostics
- Medical Imaging and Analysis
- Marine Ecology and Invasive Species
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
University of Pennsylvania
2018-2024
California University of Pennsylvania
2024
Yale University
2013-2024
American College of Cardiology
2021
ABSTRACT Shear‐thinning and self‐healing hydrogels are being investigated in various biomedical applications including drug delivery, tissue engineering, 3D bioprinting. Such formed through dynamic reversible interactions between polymers or polypeptides that allow these shear‐thinning properties, physical associations (e.g., hydrogen bonds, guest–host interactions, biorecognition motifs, hydrophobicity, electrostatics, metal–ligand coordination) covalent chemistry Schiff base, oxime...
The fabrication of three-dimensional (3D) scaffolds is indispensable to tissue engineering and 3D printing emerging as an important approach towards this. Hydrogels are often used inks in extrusion-based printing, including with encapsulated cells; however, numerous challenging requirements exist, appropriate viscosity, the ability stabilize after extrusion, cytocompatibility. Here, we present a shear-thinning self-healing hydrogel crosslinked through dynamic covalent chemistry for...
PIWI proteins and their bound PIWI-interacting RNAs (piRNAs) are found in animal germlines essential for fertility, but functions outside of the gonad not well understood. The cnidarian Hydra is a simple metazoan with well-characterized stem/progenitor cells that provides unique model analysis function. Here we report has two proteins, (Hywi) PIWI-like (Hyli), both which expressed all cells, terminally differentiated cells. We identified ∼15 million piRNAs associated Hywi and/or Hyli they...
Central artery stiffness has emerged over the past 15 years as a clinically significant indicator of cardiovascular function and initiator disease. Loss elastic fiber integrity is one primary contributors to increased arterial stiffening in aging, hypertension, related conditions. Elastic fibers consist an elastin core multiple glycoproteins; hence defects any these constituents can adversely affect wall mechanics. In this paper, we focus on mechanical consequences loss fibulin-5,...
Transforming growth factor-beta is a pleiotropic cytokine having diverse roles in vascular morphogenesis, homeostasis, and pathogenesis. Altered activity of signaling through transforming has been implicated thoracic aortic aneurysms dissections, conditions characterized by reduced structural integrity the wall that associates with altered biomechanics mechanobiology. We quantify contrast passive active biaxial biomechanical properties ascending proximal descending aorta mouse model...
Injectable hydrogels are being widely explored for treatment after myocardial infarction (MI) through mechanical bulking or the delivery of therapeutics. Despite this interest, there have been few approaches to image upon injection identify their location, volume, and pattern delivery, features that important understand toward clinical translation. Using a hyaluronic acid (HA) hydrogel as an example, aim study is introduce radiopacity by encapsulating clinically used contrast agent...
Immunotherapies have become the standard treatment for melanoma. To further improve patient responses, combinations of immunotherapies and radiotherapy (RT) are being studied, since radiotherapies can potentially provide additional immune stimulation, in addition to direct antitumor effects. FLASH-RT is a novel, ultrahigh dose rate, radiation delivery approach, with potential at least equivalent tumor control efficacy reduced damage healthy tissue. However, effects combining immunotherapy...
Abstract Background Defining epithelial cell contributions to inflammatory bowel disease (IBD) is essential for the development of much needed therapies barrier repair. Children with very early onset (VEO)-IBD have more extensive, severe, and refractory than older children adults IBD and, in some cases, defective function. We therefore evaluated functional transcriptomic differences between pediatric (VEO onset) non-IBD epithelium using 3-dimensional, biopsy-derived organoids. Methods...
Extracellular vesicles (EVs) are gaining interest in regenerative medicine and biomaterials have been shown to extend EV bioavailability following delivery. Here, we report the labeling of both hydrogels EVs better understand hydrogel design for sustained release into tissues. Shear-thinning were engineered using guest-host (i.e., adamantane-cyclodextrin) modifications hyaluronic acid (GH), as well GH with addition gelatin crosslinked via transglutaminase (GH+Gel) temporally control...
Introduction: Following myocardial infarction (MI), extracellular matrix (ECM) degradation significantly contributes to adverse left ventricular (LV) remodeling mediated by the activation of metalloproteinases (MMPs). Intramyocardial injection hydrogels that locally release MMP inhibitors are designed modulate activation, increase wall thickness, reducing stress, resulting in improved regional mechanics and function. Pressure-volume (PV) analysis is an established approach for evaluation...
Introduction: Left ventricular (LV) remodeling is a known complication from myocardial infarction (MI). Intramyocardial hydrogel (HG) delivery can potentially prevent LV post MI. We evaluated the feasibility of using multimodality imaging approach to assess and response an imageable HG in chronic porcine MI model. Methods: Nine Yorkshire pigs subjected 90 min balloon occlusion reperfusion were randomized 5 days (n=5) or controls (n=4). 99m Tc-Tetrofosmin was injected during risk area (RA)....
Myocardial infarction (MI) is often complicated by left ventricular (LV) remodeling and heart failure. We evaluated the feasibility of a multimodality imaging approach to guide delivery an imageable hydrogel assessed LV functional changes with therapy. Yorkshire pigs underwent surgical occlusions branches anterior descending and/or circumflex artery create anterolateral MI. hemodynamic mechanical effects intramyocardial in central infarct area (Hydrogel group,
Introduction: Myocardial infarction (MI) causes acute regional changes in stiffness and strain, resulting pathological remodeling the left ventricle (LV). Intramyocardial injection of hydrogel (HG) into MI region may alter deformation improve LV remodeling. Using high resolution cineCT (CCT) we evaluated global function, geometry chronic porcine model. Methods: Yorkshire pigs (n=12) subjected to 90 min balloon occlusion reperfusion were randomized 5 days post intramyocardial delivery MI+HG...
Introduction: Early studies have shown therapeutic benefits of mesenchymal stromal cells (MSCs) in cardioprotection due to their angiogenic, proliferative, anti-apoptotic and anti-inflammatory properties, which are now attributed secreted factors such as extracellular vesicles (EVs). While MSC-EVs promise small animals for cardiovascular therapies, large animal required evaluate the benefit clinical translation. One biggest challenges is need generate clinically-relevant quality quantity EVs...