A5002965034- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Autophagy in Disease and Therapy
- Legume Nitrogen Fixing Symbiosis
- Lipid Membrane Structure and Behavior
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Fungal and yeast genetics research
- Lysosomal Storage Disorders Research
- Plant biochemistry and biosynthesis
- Microtubule and mitosis dynamics
- Plant Gene Expression Analysis
- Pancreatic function and diabetes
- Plant Parasitism and Resistance
- Advanced Fluorescence Microscopy Techniques
- Phytase and its Applications
- Calcium signaling and nucleotide metabolism
Michigan State University
2016-2025
Great Lakes Bioenergy Research Center
2016-2025
John Wiley & Sons (United Kingdom)
2023
University of Oxford
2005-2023
Hudson Institute
2023
John Wiley & Sons (United States)
2023
Michigan United
2012-2020
United States Department of Energy
2015-2019
University of Wisconsin–Madison
2019
Oxford Brookes University
1999-2017
A challenging task in the study of secretory pathway is identification and localization new proteins to increase our understanding functions different organelles. Previous proteomic studies endomembrane system have been hindered by contaminating proteins, making it impossible assign Here we used organelle isotope tagging technique conjunction with tags for relative absolute quantitation 2D liquid chromatography simultaneous assignment multiple subcellular compartments. With this approach,...
Abstract Membranes are primary sites of perception environmental stimuli. Polyunsaturated fatty acids major structural constituents membranes that also function as modulators a multitude signal transduction pathways evoked by Different stresses induce production distinct blend oxygenated polyunsaturated acids, “oxylipins.” We employed three Arabidopsis (Arabidopsis thaliana) ecotypes to examine the oxylipin signature in response specific and determined wounding drought differentially alter...
Endoplasmic reticulum (ER)-mediated protein secretion and quality control have been shown to play an important role in immune responses both animals plants. In mammals, the ER membrane-located IRE1 kinase/endoribonuclease, a key regulator of unfolded response (UPR), is required for plasma cell development accommodate massive immunoglobulins. Plant cells can secrete so-called pathogenesis-related (PR) proteins with antimicrobial activities upon pathogen challenge. However, whether plays any...
The mechanisms that control protein transport between the endoplasmic reticulum (ER) and Golgi apparatus are poorly characterized in plants. Here, we examine tobacco leaves structural relationship ER membranes using electron microscopy demonstrate contain elements close association and/or direct contact with ER. We further visualized trafficking marker proteins tagged green fluorescent protein. Using photobleaching techniques, showed membrane markers constitutively cycle to from an...
In contrast with animals, plant cells contain multiple mobile Golgi stacks distributed over the entire cytoplasm. However, distribution and dynamics of protein export sites on endoplasmic reticulum (ER) surface have yet to be characterized. A widely accepted model for ER-to-Golgi transport is based sequential action COPII COPI coat complexes. The complex assembles by ordered recruitment cytosolic components ER membrane. Here, we visualized two early machinery, small GTPase Sar1p its GTP...
In plants, heat stress responses are controlled by transcription factors that conserved among all eukaryotes and can be constitutively expressed or induced heat. Heat-inducible distinct from the "classical" have also been reported to contribute tolerance. Here, we show bZIP28, a gene encoding putative membrane-tethered factor, is up-regulated in response bZIP28 null mutant has striking heat-sensitive phenotype. The heat-inducible expression of genes encode BiP2, an endoplasmic reticulum (ER)...
We have fused the signal anchor sequences of a rat sialyl transferase and human galactosyl along with Arabidopsis homologue yeast HDEL receptor (AtERD2) to jellyfish green fluorescent protein (GFP) transiently expressed chimeric genes in tobacco leaves. All constructs targeted Golgi apparatus co-expression DsRed fusions immunolabelling stably transformed BY2 cells indicated that fusion proteins located all stacks. Exposure tissue brefeldin A (BFA) resulted reversible redistribution ST-GFP...
The tonoplast was proposed as a default destination of membrane-bound proteins without specific targeting signals. To investigate the nature this targeting, we created type I fusion with green fluorescent protein followed by transmembrane domain human lysosomal LAMP1. We varied length from 23 to either 20 or 17 amino acids deletion within hydrophobic domain. resulting chimeras, called TM23, TM20, and TM17, were expressed transiently stably in tobacco. TM23 clearly accumulated plasmalemma,...
We have characterized the requirements to inhibit function of plant vacuolar sorting receptor BP80 in vivo and gained insight into crucial role recycling between prevacuolar compartment Golgi apparatus. The drug wortmannin interferes with BP80-mediated route vacuole induces hypersecretion a soluble BP80-ligand. Wortmannin does not prevent receptor-ligand binding itself but causes levels be limiting. Consequently, overexpression partially restores cargo transport. To simulate traffic, we...
Over the past few years, a vast amount of research has illuminated workings secretory system eukaryotic cells. The bulk this work been focused on yeast Saccharomyces cerevisiae, or mammalian At superficial level, plants are typical eukaryotes with respect to operation system; however, important differences emerge in function and appearance endomembrane organelles. In particular, plant specialized several ways support synthesis many components complex cell wall, kinds vacuole have taken...
Tocopherols are nonpolar compounds synthesized and localized in plastids but whose genetic elimination specifically impacts fatty acid desaturation the endoplasmic reticulum (ER), suggesting a direct interaction with ER-resident enzymes. To functionally probe for such interactions, we developed transorganellar complementation, where mutated pathway activities one organelle experimentally tested substrate accessibility complementation by active enzymes retargeted to companion organelle....
Arabidopsis thaliana calmodulin binding transcription activator (CAMTA) factors repress the expression of genes involved in salicylic acid (SA) biosynthesis and SA-mediated immunity healthy plants grown at warm temperature (22°C). This repression is overcome exposed to low (4°C) for more than a week infected by biotrophic hemibiotrophic pathogens. Here, we present evidence that CAMTA3-mediated SA pathway nonstressed involves action an N-terminal module (NRM) acts independently (CaM) IQ CaM...
Summary The endoplasmic reticulum (ER) has the ability to maintain balance between demand for and synthesis of secretory proteins. To ensure protein‐folding homeostasis in ER, cells invoke signaling pathways known as unfolded protein response (UPR). initiate UPR, yeasts largely rely on a conserved sensor, IRE1. In metazoans, there are at least three independent UPR signalling pathways. Some transducers have been identified plants, but no genetic interaction among them yet examined....
In plant cells, the endoplasmic reticulum (ER) and Golgi apparatus form a unique system in which single stacks are motile close association with underlying ER tubules. Arabidopsis has three RHD3 (ROOT HAIR DEFECTIVE 3) isoforms that analogous to mammalian atlastin GTPases involved shaping We used live-cell imaging, genetic complementation, split ubiquitin assays western blot analyses tobacco show mediates generation of tubular network is required for distribution motility root leaf epidermal...
The unfolded protein response (UPR) signaling network encompasses two pathways in plants, one mediated by inositol-requiring protein-1 (IRE1)-bZIP60 mRNA and the other site-1/site-2 proteases (S1P/S2P)-bZIP17/bZIP28. As major sensor of UPR eukaryotes, IRE1, to endoplasmic reticulum (ER) stress, catalyzes unconventional splicing HAC1 yeast, bZIP60 plants XBP1 metazoans. Recent studies suggest that IRE1p mRNA, only pathway found evolves as a cognate system responsible for robust induction....
Viruses recruit cellular membranes and subvert proteins involved in lipid biosynthesis to build viral replicase complexes replication organelles. Among the lipids, sterols are important components of membranes, affecting shape curvature membranes. In this paper, tombusvirus protein is shown co-opt Oxysterol-binding related (ORPs), whose deletion yeast model host leads decreased replication. addition, tombusviruses also Scs2p VAP facilitate formation membrane contact sites (MCSs), where...
Abstract Xyloglucan is a polysaccharide that has important roles in the formation and function of walls surround growing land plant cells. Many these plants synthesize xyloglucan contains galactose two different side chains (L F), which exist distinct molecular environments. However, little known about contribution to function. Here, we show Arabidopsis (Arabidopsis thaliana) mutants devoid F chain galactosyltransferase MURUS3 (MUR3) form lacks much less galactosylated xylose than its...
The target of rapamycin (TOR) kinase is an evolutionarily conserved hub nutrient sensing and metabolic signaling. In plants, a functional connection TOR activation with glucose availability was demonstrated, while it yet unclear whether branched-chain amino acids (BCAAs) are primary input signaling as they in yeast mammalian cells. Here, we report on the characterization Arabidopsis mutant over-accumulating BCAAs. Through chemical interventions targeting by examining mutants BCAA...
Significance Plant cells have a polysaccharide-based wall that maintains their structural and functional integrity determines shape. Reorganization of components is required to allow growth differentiation. One matrix polysaccharide postulated play an important role in this reorganization xyloglucan (XyG). While the structure XyG well understood, its biosynthesis not. Through genetic studies with Arabidopsis CSLC genes, we demonstrate they are responsible for synthesis glucan backbone. A...
The unfolded protein response (UPR) is a highly conserved that protects plants from adverse environmental conditions. UPR elicited by endoplasmic reticulum (ER) stress, in which and misfolded proteins accumulate within the ER. Here, we induced maize (
Plants produce phylogenetically and spatially restricted, as well structurally diverse specialized metabolites via multistep metabolic pathways. Hallmarks of evolution include enzymatic promiscuity recruitment primary enzymes examples genomic clustering pathway genes. Solanaceae glandular trichomes defensive acylsugars, with sidechains that vary in length across the family. We describe a tomato gene cluster on chromosome 7 involved medium chain acylsugar accumulation due to trichome specific...