A5102825947- Gastrointestinal Tumor Research and Treatment
- Gastric Cancer Management and Outcomes
- Epigenetics and DNA Methylation
- Metastasis and carcinoma case studies
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Gastrointestinal disorders and treatments
- Esophageal and GI Pathology
- Circular RNAs in diseases
- Pancreatic and Hepatic Oncology Research
- Eosinophilic Esophagitis
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Helicobacter pylori-related gastroenterology studies
- Gastroesophageal reflux and treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related molecular mechanisms research
- Soft tissue tumor case studies
- Cancer Cells and Metastasis
- Foreign Body Medical Cases
- Dysphagia Assessment and Management
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
Fudan University
2015-2024
Zhongshan Hospital
2015-2024
South China Institute of Collaborative Innovation
2022
Ministry of Education of the People's Republic of China
2018-2020
Sun Yat-sen University
2019
The First Affiliated Hospital, Sun Yat-sen University
2019
Cancer Institute (WIA)
2018
Objective The lack of highly sensitive and specific diagnostic biomarkers is a major contributor to the poor outcomes patients with hepatocellular carcinoma (HCC). We sought develop non-invasive approach using circulating cell-free DNA (cfDNA) for early detection HCC. Design Applying 5hmC-Seal technique, we obtained genome-wide 5-hydroxymethylcytosines (5hmC) in cfDNA samples from 2554 Chinese subjects: 1204 HCC, 392 chronic hepatitis B virus infection (CHB) or liver cirrhosis (LC) 958...
Recently, the dysregulation of circular RNA (circRNA) have been shown to important regulatory roles in cancer development and progression, including hepatocellular carcinoma (HCC). However, most circRNAs HCC are still unknown.The expression tripartite motif containing 33-12 (circTRIM33-12) tissues cell lines was detected by qRT-PCR. The role circTRIM33-12 progression assessed western blotting, CCK-8, flow cytometry, transwell a subcutaneous tumor mouse assays both vitro vivo. In vivo circRNA...
Ratiometric biosensors employing Förster Resonance Energy Transfer (FRET) enable the real-time tracking of metabolite dynamics. Here, we introduce an approach for generating a FRET-based biosensor in which changes apparent FRET efficiency rely on analyte-controlled fluorogenicity rhodamine rather than commonly used distance change between donor-acceptor fluorophores. Our fluorogenic, rhodamine-based, chemigenetic (
Abstract Lymphoid‐specific helicase (LSH) is overexpressed in tumor tissues and its overexpression associated with poor prognosis several cancers. However, the role molecular mechanism of LSH hepatocellular carcinoma (HCC) remains largely unknown. Herein, we report that was HCC, from a public HCC database, validated by clinical samples our department. Ectopic expression promoted growth cells vivo vitro. Mechanistically, activating transcription centromere protein F (CENPF). Clinically,...
Sorafenib, a multikinase inhibitor, is new standard treatment for patients with advanced hepatocellular carcinoma (HCC).However, resistance to this regimen frequently observed in clinical practice, and the molecular basis of remains largely unknown.Herein, antitumor activity sorafenib was assessed 16 patient-derived xenograft (PDX) models HCC.Gene expression analysis conducted identify factors that promote resistance.Quantitative RT-PCR immunoblotting were used determine gene activation...
The molecular pathogenesis of intrahepatic cholangiocarcinoma (iCCA) is poorly understood, and its incidence continues to increase worldwide. Deficiency mitogen‐activated protein kinase 4 (MAP3K4) has been reported induce the epithelial‐mesenchymal transition (EMT) process placental embryonic development, yet role in human cancer remains unknown. MAP3K4 somatic mutation iCCA so we sequenced all exons 124 patients. We identified nine mutations 10 (8.06%) patients, especially those with lymph...
Abstract Hepatocellular carcinoma (HCC) is closely associated with abnormal DNA methylation. In this study, we analyzed 450K methylation chip data from 377 HCC samples and 50 adjacent normal in the TCGA database. We screened 47,099 differentially methylated sites using Cox regression as well SVM‐RFE FW‐SVM algorithms, constructed a model three risk categories to predict overall survival based on 134 sites. The showed 10‐fold cross‐validation score of 0.95 satisfactory predictive power,...
Abstract 5‐Hydroxymethylcytosine (5hmC) is a DNA modification that generated by the oxidation of 5‐methylcytosine (5mC) in reaction catalyzed ten‐eleven translocation (TET) family enzymes. It tends to mark gene activation and affects spectrum developmental disease‐related biological processes. In this manuscript, we present 5hmC selective chemical labelling technology (hmC‐Seal) capture sequence 5hmC‐containing fragments with low input. We tested 10 tumour/adjacent colon cancer tissues...
Background Postoperative extrahepatic metastases (EHM) contribute to a grim outcome in patients with hepatocellular carcinoma (HCC) who are undergoing curative surgical resection. The current study investigated the clinical value of circulating tumor cells (CTCs) predicting EHM after surgery. Methods A total 197 HCC were resection assigned retrospective training cohort (144 patients) or prospective validation (53 patients). CELLSEARCH system was used for detection CTCs prior and 1 month...
Achalasia is a rare motility disorder of the esophagus caused by gradual degeneration myenteric neurons. Immune-mediated ganglionitis has been proposed to underlie loss Here, we measure immune cell transcriptional profile paired lower esophageal sphincter (LES) tissue and blood samples in achalasia controls using single-cell RNA sequencing (scRNA-seq). In achalasia, identify pattern expanded cells specific phenotype, especially LES tissue. We show C1QC+ macrophages tissue-resident memory T...
// Ai-Wu Ke 1, * , Peng-Fei Zhang Ying-Hao Shen Ping-Ting Gao Zhao-Ru Dong Chi Jia-Bin Cai 1 Xiao-Yong Huang Chao Wu 2 Lu Qiang Kang 3 Li-Xin Liu Nan Xie Zao-Zhuo Mei-Yu Hu Ya Cao 4 Shuang-Jian Qiu Hui-Chuan Sun Jian Zhou Jia Fan Guo-Ming Shi Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Invasion (Fudan University), Ministry Education, Shanghai 200032, P.R. China Department Hepatobiliary Surgery, Subei People's Yangzhou 225000, Second...
The approach to paraoesophageal structures and lesions via the oesophagus was initiated by introduction of peroral endoscopic myotomy (POEM), followed removal tumours within muscular layer. We now report a case which suggests that next step, is, remove not related oesophageal wall, would also be possible. This is 66-year-old male patient with 4.0×2.0×1.5 cm benign schwannoma from recurrent laryngeal nerve bulging into proximal oesophagus, we removed POEM tunnel. postoperative course...
The spectrum of endoscopic resection methods, including full thickness techniques, has enabled removal most smaller gastric submucosal tumours (SMTs). We developed an extended transmural dissection technique for lesions with predominately extraluminal growth arising from the muscularis propria. It consists intraperitoneal access endoscope besides lesion followed by subserosal outside. was successful in two patients (2–3 cm) stromal tumours; were resected completely and short-term follow-up...
Achalasia is characterized by impaired swallowing due to lower esophageal sphincter (LES) dysfunction and an increased risk of carcinoma. Aflatoxin a known carcinogen. Esophageal retention relieved per oral endoscopic myotomy (POEM), which lowers the cancer risk. The present study determined whether aflatoxin involved in pathogenesis achalasia or cancer. A total 75 patients with were prospectively enrolled from tertiary center. levels their contents measured using ELISA, mucosal specimens...