A5102193846- Pneumonia and Respiratory Infections
- Microbial infections and disease research
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Antibiotics Pharmacokinetics and Efficacy
- Semiconductor materials and devices
- Gut microbiota and health
- Vibrio bacteria research studies
- Genetics and Physical Performance
- Blood groups and transfusion
- Forensic and Genetic Research
- Silicon Carbide Semiconductor Technologies
- Medical Malpractice and Liability Issues
- Monoclonal and Polyclonal Antibodies Research
- Liver Disease Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Infections and bacterial resistance
- Bacterial biofilms and quorum sensing
- Health disparities and outcomes
- Alcohol Consumption and Health Effects
- 14-3-3 protein interactions
- Bacterial Infections and Vaccines
- Air Quality and Health Impacts
- Bacterial Identification and Susceptibility Testing
Beijing Information Science & Technology University
2025
Capital Institute of Pediatrics
2014-2024
University of Electronic Science and Technology of China
2023-2024
First Affiliated Hospital of Jiangxi Medical College
2024
Nanchang University
2024
National Engineering Research Center of Electromagnetic Radiation Control Materials
2024
Fudan University
2024
Zhongshan Hospital
2024
State Key Laboratory of Electronic Thin Films and Integrated Devices
2024
Beijing University of Agriculture
2023-2024
Our previous studies have shown that high alcohol-producing Klebsiella pneumoniae (HiAlc Kpn) in the intestinal microbiome could be one of causes non-alcoholic fatty liver disease (NAFLD). Considering antimicrobial resistance K. and dysbacteriosis caused by antibiotics, phage therapy might potential treatment HiAlc Kpn-induced NAFLD, because specificity targeting bacteria. Here, we clarified effectiveness male mice with steatohepatitis. Comprehensive investigations including transcriptomes...
Pneumoconiosis remains a major global occupational health hazard and illness. Accurate data on the incidence of pneumoconiosis are critical for resource planning development policy.We collected period between 1990 2017 annual incident cases age-standardised rates (ASIR) aetiology from Global Burden Disease Study 2017. We calculated average percentage changes ASIR by sex, region in order to determine trends pneumoconiosis.Globally, number increased measure 66.0%, 36 186 60 055 The overall...
A novel coronavirus (SARS-CoV-2) was recently identified in patients with acute respiratory disease and spread quickly worldwide. specific rapid diagnostic method is important for early identification. The reverse-transcription recombinase-aided amplification (RT-RAA) assay a detection several pathogens. Assays were performed within 5–15 min as one-step single tube reaction at 39 °C. In this study, we established two RT-RAA assays the S orf1ab gene of SARS-CoV-2 using clinical specimens...
Monkeypox virus (MPXV) is a human pathogenic that belongs to the genus Orthopoxvirus. In 2022, MPXV caused an unprecedented number of infections in many countries. As it difficult distinguish from other pathogens by its symptoms early stage infection, rapid and reliable assay for detection needed. this study, we developed loop-mediated isothermal amplification (LAMP) specific evaluated application simulated clinical samples. The A27L-1 F3L-1 primer sets were identified as optimal primers,...
To analyze the molecular and clinical characteristics of Mycoplasma pneumoniae (Mp) pneumonia (MPP) to explore related factors predicting severe MPP (SMPP).A total 423 pediatric cases were retrospectively analyzed, in 2013-2017, Beijing, China. Clinical information was collected from medical records. Mp-positive specimens characterized using P1 typing multiple locus variable-number tandem repeat analysis (MLVA). The macrolide resistance-associated mutations also detected.The predominant...
Ratiometric biosensors employing Förster Resonance Energy Transfer (FRET) enable the real-time tracking of metabolite dynamics. Here, we introduce an approach for generating a FRET-based biosensor in which changes apparent FRET efficiency rely on analyte-controlled fluorogenicity rhodamine rather than commonly used distance change between donor-acceptor fluorophores. Our fluorogenic, rhodamine-based, chemigenetic (
Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) currently most discriminative method for typing M. strains. To better understand epidemic pneumoniae-related pediatric patients Beijing, China, we performed MLVA on 118 specimens collected during an from 2010–2012. Eleven distinct types were identified, including four novel types. There was no obvious association macrolide...
Medical litigation represents a growing cost to healthcare systems. Mediation, arbitration, and other alternative dispute resolution (ADR) methods are increasingly used help solve the disputes improve satisfaction. In China, increasing number of medical has contributed concern for safety physicians mistrust between physician patients resulting in ADR processes being established several provinces recent years. Our aim was describe explain impact this new mediation process Chinese system.Our...
High alcohol-producing Klebsiella pneumoniae (HiAlc Kpn) in the gut microbiota had been demonstrated to be causative agent of fatty liver disease (FLD). However, catabolic pathways for alcohol production vivo remain unclear. Here, we characterized genome HiAlc and medium (MedAlc) Kpn constructed an adh (an essential gene encoding dehydrogenase) knock-out W14 strain (W14Δadh) using CRISPR-Cas9 system. Subsequently, established mouse model via gavage administration Δadh strains, respectively....
Pneumonia caused by multidrug-resistant (MDR) Klebsiella pneumoniae of sequence types ST11 and ST383 have highlighted the necessity for new therapies against these prevalent pathogens. Bacteriophages (phages) may be used as alternatives or complements to antibiotics treating MDR bacteria because they show potential efficacy in mouse models even individual clinical cases, also cause fewer side effects, such microbiota-imbalance-induced diseases. In present study, we screened two phages, pKp11...
This study aimed to develop a rapid and sensitive droplet digital PCR (ddPCR) assay for the specific detection of Klebsiella pneumoniae in fecal samples, evaluate its application clinic by comparison with real-time conventional microbial culture. Specific primers probe targeting K. hemolysin (khe) gene were designed. Thirteen other pathogens used specificity probe. A recombinant plasmid containing khe was constructed assess sensitivity, repeatability, reproducibility ddPCR. Clinical samples...
Abstract Background Mycoplasma pneumoniae is one of the most common causative pathogens community-acquired pneumonia (CAP), accounting for as many 30–50% CAP during peak years. An early and rapid diagnostic method key guiding clinicians in their choice antibiotics . Methods The recombinase-aided amplification (RAA) assay a recently developed, detection that has been used several pathogens. assays were performed one-step single tube reaction at 39° Celsius within 15–30 min. In this study, we...
Leptin has been found to be involved in the development and progression of many autoimmune diseases. As an organ-specific disease, pathogenesis Hashimoto’s thyroiditis not fully elucidated. It reported that serum leptin level is increased thyroiditis, but other studies have shown any difference. We replicated a mouse model experimental (EAT) with high-iodine diet injection receptor antagonist Allo-aca reduced thyroid follicle destruction inflammatory cell infiltration EAT mice, thyroxine...
Abstract Background Klebsiella aerogenes can cause ventilator-associated pneumonia by forming biofilms, and it is frequently associated with multidrug resistance. Phages are good antibiotic alternatives unique advantages. There has been a lack of phage therapeutic explorations, kinetic studies, interaction mechanism research targeting K. . Methods Plaque assay, transmission electron microscopy whole-genome sequencing were used to determine the biology, morphology, genomic characteristics...
Mycoplasma pneumoniae is usually susceptible to macrolides, but macrolide-resistant strains have been found frequently in recent years. Mutations domain V of the 23S rRNA gene M. interfere with binding macrolides and mediate macrolide resistance. In this study, we developed a rapid inexpensive method that combines nested PCR (nPCR), single-strand conformation polymorphisms (SSCPs), capillary electrophoresis (CE) detect mutants directly from throat swabs. nPCR was used specifically amplify...
The molecular characteristics of 480 Mycoplasma pneumoniae polymerase chain reaction-positive specimens (331 were previously reported and 149 newly reported) collected from pediatric patients in Beijing, China, between 2003 2015 analyzed. Genotype M4-5-7-2/P1 the most prevalent across 13-year study period, although isolation mutation rates for this genotype varied periods 2003–2007, 2008–2013, 2014–2015. In addition, there was a close association M4-5-7-2 macrolide resistance.
A recent, unprecedented outbreak of human mpox virus infection has led to cases in non-African nations, and the number confirmed or suspected outside Africa exceeded 1,000 within 5 weeks. Mpox may pose a double threat public health context ongoing COVID-19 pandemic. It is difficult distinguish from other diseases early stages, patients are contagious onset nonspecific symptoms; therefore, it crucial develop rapid specific diagnostic methods. The diagnosis relies on real-time polymerase chain...