A5100725106- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Genetics, Bioinformatics, and Biomedical Research
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- T-cell and B-cell Immunology
- Biomedical Text Mining and Ontologies
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Bacteriophages and microbial interactions
- Eicosanoids and Hypertension Pharmacology
Shanghai University of Medicine and Health Sciences
2018-2021
Shanghai Tenth People's Hospital
2018
Tongji University
2018
Columbia University Irving Medical Center
1994
New York University
1994
Objective The lack of highly sensitive and specific diagnostic biomarkers is a major contributor to the poor outcomes patients with hepatocellular carcinoma (HCC). We sought develop non-invasive approach using circulating cell-free DNA (cfDNA) for early detection HCC. Design Applying 5hmC-Seal technique, we obtained genome-wide 5-hydroxymethylcytosines (5hmC) in cfDNA samples from 2554 Chinese subjects: 1204 HCC, 392 chronic hepatitis B virus infection (CHB) or liver cirrhosis (LC) 958...
This study aimed to establish a quantitative droplet-digital polymerase chain reaction (ddPCR) detection system for clinical hepatitis B virus (HBV) DNA in samples from patients with HBV infection the aim of monitoring fluctuations and planning future antiviral treatments. Primers probes conserved region sequence (GenBank: AB554017.1) were designed by alignment GenBank. The PUC57 plasmid containing gene fragment was then synthesized according amplified primer. Linear templates obtained...
Abstract Background Recently, more and studies have highlighted the critical regulatory roles of circular RNAs (circRNAs), a class non-coding RNAs, in progression many human cancers, including prostate cancer (PCa). circRNA microarray analysis was performed to identify circRNAs that are differentially expressed PCa tissues. Methods 104 pairs tissues matched adjacent normal (at least 2 cm distal tumor margin) were obtained. on four investigate potential involvement PCa. Flow cytometric...
Abstract The lack of highly sensitive and specific diagnostic biomarkers is a major contributor to the poor outcomes patients with hepatocellular carcinoma (HCC), second-most common cause cancer deaths worldwide. We sought develop clinically convenient minimally-invasive approach that can be deployed at scale for sensitive, specific, reliable diagnosis HCC, evaluate potential prognostic value this approach. study cohort comprised 2,728 subjects, including HCC (n = 1,208), controls 965) (572...
Objective: This study aimed to identify the novel microRNAs (miRNAs) for early diagnosis of bladder cancer. Materials and Methods: Differentially expressed miRNAs between advanced cancer were identified by differential expression analysis, using miRNA-seq data from The Cancer Genome Atlas (TCGA). optimal subset feature pathologic stage prediction was acquired Random Forest algorithm used construct a support vector machine (SVM) classifier. performance SVM classifier in predicting progression...
Molecular diagnosis plays more and important role in disease diagnosis, prognosis evaluation curative effect monitoring. With the support of relevant national policies gradual release accurate medical needs from society, molecular relies on its advantages accuracy, convenience, sensitivity, non-invasiveness automation to develop rapidly direction precision inspection. The application diagnostic is undoubtedly catalyst for development diagnostics, but status far satisfactory. It necessary...