A5102887448- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- Circular RNAs in diseases
- Immune cells in cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver physiology and pathology
- FOXO transcription factor regulation
- Cancer, Lipids, and Metabolism
- Photonic and Optical Devices
- Peptidase Inhibition and Analysis
- Semiconductor Lasers and Optical Devices
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- PARP inhibition in cancer therapy
- RNA Interference and Gene Delivery
- Curcumin's Biomedical Applications
- Adenosine and Purinergic Signaling
Zhongshan Hospital
2018-2024
Fudan University
1990-2024
Advanced Semiconductor Engineering (Taiwan)
2024
National Cheng Kung University
2023
Mayo Clinic
2023
Mayo Clinic Hospital
2023
Ministry of Education of the People's Republic of China
2018-2020
The overall response rate for anti-PD-1 therapy remains modest in hepatocellular carcinoma (HCC). We found that a combination of IFNα and anti-PD-1-based immunotherapy resulted enhanced antitumor activity patients with unresectable HCC. In both immunocompetent orthotopic spontaneous HCC models, synergized the treatment led to significant enrichment cytotoxic CD27+CD8+ T cells. Mechanistically, suppressed HIF1α signaling by inhibiting FosB transcription cells, resulting reduced glucose...
Guanosine triphosphate binding protein 4 (GTPBP4) is a key regulator of cell cycle progression and MAPK activation. However, how its biological properties intersect with cellular metabolism in hepatocellular carcinoma (HCC) development remains poorly unexplained. Here, high GTPBP4 expression found to be significantly associated worse clinical outcomes patients HCC. Moreover, upregulation paralleled by DNA promoter hypomethylation regulated DNMT3A, methyltransferase. Additionally, both gain-...
Cholangiocarcinoma was a highly malignant liver cancer with poor prognosis, and immune infiltration status considered an important factor in response to immunotherapy. In this investigation, we tried locate related genes of cholangiocarcinoma through combination bulk-sequencing single-cell sequencing technology. Single sample gene set enrichment analysis used annotate datasets TCGA CHOL, GSE32225, GSE26566. Differentially expressed between high- low-infiltrated groups dataset were yielded...
A TLR9 agonist in combination with a PD-1 inhibitor produced powerful antitumor responses clinical trial despite agonists as monotherapies failing to generate systemic immune due immunosuppressive effects.However, the mechanism involved improved response induced by their remains unknown.Methods: Subcutaneous and orthotopic Hepa1-6 tumor model was used for single-drug combined-drug treatment.We stimulation or lentiviral vectors overexpress activate signaling.We next investigated crosstalk...
<p>Supplementary materials and methods, Supplementary Figures figure legends, Tables</p>
<div>Abstract<p>Anti–PD-L1–based combination immunotherapy has become the first-line treatment for unresectable hepatocellular carcinoma (HCC). However, objective response rate is lower than 40%, highlighting need to identify mechanisms of tolerance immune checkpoint inhibitors and accurate biomarkers response. In this study, we used next-generation sequencing analyze HCC samples from 10 patients receiving anti–PD-L1 therapy. Activation renin–angiotensin system was elevated in...
Abstract The aim of this study was to identify novel prognostic mRNA and microRNA (miRNA) biomarkers for hepatocellular carcinoma (HCC) using methods in systems biology. Differentially expressed mRNAs, miRNAs, long non-coding RNAs (lncRNAs) were compared between HCC tumor tissues normal liver Cancer Genome Atlas (TCGA) database. Subsequently, a prognosis-associated co-expression network, an mRNA–miRNA regulatory mRNA–miRNA–lncRNA network constructed through Cox survival analysis. Seven...
Autophagy in tumor was also found to influence immune microenvironment. The relation between autophagy and cancer intrinsic PD1 PD-L1 expression not clear.With data from TCGA GTEx databases, mRNA levels of autophagy-related genes were compared samples normal tissues, which correlated with survival status. Expression associated clinical traits datasets GSE14520 ICGC LIRI. Single sample gene set enrichment analysis (ssGSEA) used calculate scores samples, using signatures MSigDB database....
Prognosis of hepatocellular carcinoma (HCC) remains poor due to high recurrence rate and ineffective treatment options, highlighting the need better understand mechanism metastasis in HCC.We first collected messenger RNA (mRNA) expression data from 442 cases HCC patients The Cancer Genome Atlas (TCGA) database as well 251 Zhongshan Hospital during 2009 2010 analyze pattern tissue microarray (TMA) baculoviral IAP repeat containing 3 (BIRC3). Then, we used BIRC3 gain-of-function...
Abstract Circular RNAs (circRNAs) are a class of regulatory with complex roles in healthy and diseased tissues. However, the oncogenic role circRNAs hepatocellular carcinoma (HCC) remains poorly understood, including mechanisms by which circular ubiquitin-binding associated protein 2 (circUBAP2) contributes to tumorigenesis. We analyzed expression circUBAP2 20 paired samples HCC tissue as well seven cell lines via quantitative real-time polymerase chain reaction. Functional experiments, such...
Abstract Anti-PD-L1-based combination immunotherapy has become the first-line treatment for unresectable hepatocellular carcinoma (HCC). However, objective response rate is lower than 40%, highlighting need to identify mechanisms of tolerance immune checkpoint inhibitors and accurate biomarkers response. Here, we employed next-generation sequencing analyze HCC samples from 10 patients receiving anti-PD-L1 therapy. Activation renin-angiotensin system was elevated in nonresponders compared...
Functional roles of SIGLEC15 in hepatocellular carcinoma (HCC) were not clear, which was recently found to be an immune inhibitor with similar structure inhibitory B7 family members. expression HCC explored public databases and further examined by PCR analysis. PD-L1 patterns samples through immunohistochemistry. knocked-down or over-expressed cell lines, CCK8 tests used examine proliferative ability vitro. Influences on tumor growth deficient immunocompetent mice respectively. Co-culture...
There are some controversies about the involvement of microRNA (miR)-19a-3p in hepatocellular carcinoma (HCC) biology, even though many studies have shown that it plays an important role cancer. In this study, we found miR-19a-3p is usually overexpressed HCC tissues compared with corresponding peritumorous tissues, and its expression was associated tumor size poor overall survival. MiR-19a-3p promoted cell proliferation significantly, more cells were S phase. vivo, liver growth, active...
SIGLEC family genes can also be expressed on tumor cells in different cancer types, and though it has been found that by immune exploited tumors to escape surveillance, functions of derived expression microenvironment (TME) were barely investigated, which could play roles patients' survival. Using bioinformatic analysis, mutation status was explored through the cBioPortal database, them UALCAN database. The GEPIA database used compare genes' mRNA between cancers generate a highly correlated...
Cell adhesion molecule cluster of differentiation 44 (CD44) plays a significant role in cancer cell local invasion, intravasation, migration, and the establishment metastatic lesions. However, little is known about underlying mechanism how CD44 regulates hepatocellular carcinoma (HCC) extrahepatic metastasis (EHM).The expression HCC tissues lines was detected through western blot immunohistochemistry (IHC). Through gain- loss-of-function assays, we examined oncogenic roles regulating growth...
Abstract Sorafenib (SF), a multi‐kinase inhibitor, is the first FDA‐approved systemic chemotherapy drug for advanced hepatocellular carcinoma (HCC). However, its clinical application limited by severe toxicity and side effects associated with high applied doses. Sophora alopecuroides L. traditionally used as Chinese herbal medicine treating gastrointestinal diseases, bacillary dysentery, viral hepatitis, other exerts an important role in anti‐tumor. Hence, we investigated synergistic actions...
Abstract Previous research suggests that far upstream element-binding protein 1 (FUBP1) plays an important role in various tumors including epatocellular carcinoma (HCC). However, the of FUBP1 liver cancer remains controversial, and regulatory pathway by awaits to be determined. This study aims identify HCC progression. Our result shows high level expression predicts poor prognosis after surgery. Overexpression promotes proliferation, invasion, metastasis activating transforming growth...
Colorectal cancer (CRC) is one of the most prominent malignant diseases, with a high incidence and dismal prognosis. Metastasis to liver leading cause death in CRC patients. This study aimed identify accurate metastatic biomarkers investigate potential molecular mechanisms metastasis colorectal (LMCRC).Three independent datasets were screened downloaded from Gene Expression Omnibus (GEO) database. The GEO2R tool was used differentially expressed genes (DEGs) tissues metastases. Next,...
Background: Liver metastasis (LM) accounts for most colorectal cancer (CRC)-related deaths. However, how metastatic CRC cells gain the ability to survive and grow in liver remains largely unknown. Methods: First, we screened differentially expressed genes (DEGs) between LM paired primary tumors (PTs) Gene Expression Omnibus (GEO) database, identified cytochrome P450 1B1 (CYP1B1) as only common differential gene. Then, verified messenger RNA (mRNA) protein expression level clinical specimens....