A5100643580- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- HER2/EGFR in Cancer Research
- Catalytic C–H Functionalization Methods
- Natural product bioactivities and synthesis
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Cancer-related gene regulation
- BRCA gene mutations in cancer
- NF-κB Signaling Pathways
- Traditional Chinese Medicine Analysis
- Synthesis and Catalytic Reactions
- Cyclopropane Reaction Mechanisms
- Advanced Breast Cancer Therapies
- Pharmacological Effects of Natural Compounds
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Synthetic Organic Chemistry Methods
- Single-cell and spatial transcriptomics
- Kruppel-like factors research
Nantong University
2025
Chinese University of Hong Kong
2019-2024
University of Hong Kong
2018-2024
Xiangya Hospital Central South University
2009-2024
Central South University
2009-2024
Zhongshan Hospital
2020-2023
Fudan University
2017-2023
Hunan Cancer Hospital
2021-2023
Guangzhou University
2022
Cancer Institute (WIA)
2020-2021
Hepatocellular carcinoma (HCC) is a kind of complicated disease with an increasing incidence all over the world. A classic Chinese Medicine formula Zuojin Pill (ZJP) was shown to exert therapeutic effects on HCC. However, its chemical and pharmacological profiles remain be elucidated. In current study, network pharmacology approach applied characterize action mechanism ZJP All compounds were obtained from corresponding databases active selected according their oral bioavailability...
Abstract Dissecting and understanding the cancer ecosystem, especially that around tumor margins, which have strong implications for cell infiltration invasion, are essential exploring mechanisms of metastasis developing effective new treatments. Using a novel border scanning digitization model enabled by nanoscale resolution-SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq), we identified 500 µm-wide zone centered in patients with liver cancer, referred to as “the invasive zone”. We...
Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide with high mortality. Circulating miRNA has been demonstrated as a novel noninvasive biomarker for many tumors. This study aimed to investigate potential of circulating miR-125b prognostic marker HCC. Exosomes were extracted from serum samples collected two independent cohorts: cohort 1: HCC (n=30), chronic hepatitis B (CHB, n=30), liver cirrhosis (LC, n=30); 2: (n=128). We found that levels remarkably increased in...
Hepatocellular carcinoma (HCC) is a major cause of cancer-related death worldwide.Although it has been known that hepatic stellate cells (HSCs) play critical roles in the development and progression HCC, molecular mechanism underlying crosstalk between HSCs cancer still remains unclear.Here, we investigated interactions through an indirect co-culture system.The expressions cellular exosomal miR-148a-3p were evaluated by quantitative real-time PCR.Cell counting kit-8 was used for evaluating...
Background and Purpose As a typical hypervascular tumour, hepatocellular carcinoma (HCC) is predominantly grown through angiogenesis. Geniposide promising anti‐inflammatory compound found in Gardenia jasminoides , but its effects on the progression of HCC remain untested. Experimental Approach The anti‐HCC geniposide was investigated cellular models orthotopic mice. Transcriptional regulation VEGF promoter measured by dual‐luciferase reporter assay. anti‐angiogenic action tube formation Both...
Abstract Altered energy metabolism of cancer cells shapes the immune cell response in tumor microenvironment that facilitates progression. Herein, we reported novel cell-expressed Piwi Like RNA-Mediated Gene Silencing 1 (PIWIL1) mediating crosstalk fatty acid and human hepatocellular carcinoma (HCC). PIWIL1 expression HCC was increased compared to normal hepatic tissues positively correlated with proliferation rate lines. overexpression accelerated vitro vivo growth tumors, while knockdown...
Abstract Betulinic acid (BA) is a natural product extracted from broad range of medicinal and edible herbal plants. Previous studies showed that BA induces cell death in tumors derived multiple tissues; however, the underlying mechanism remains obscure. The present study aimed to effects on autophagy apoptosis hepatocellular carcinoma (HCC). Human HCC lines orthotopic implanted mice were employed examine BA‐induced tumor suppression; RT 2 long noncoding RNA (lncRNA) PCR array database...
Triple negative breast cancer (TNBC) is resistant to conventional chemotherapy due high proportions of stem cells (CSCs). The aim this study unravel the miR-137-mediated regulatory mechanism B-cell lymphoma/leukemia 11A (BCL11A) in TNBC.A corhort 34 TNBC tumor tissues and paired adjacent normal tissues, as well 25 non-TNBC were collected post-operatively from patients with cancer. Q-PCR was performed determine mRNA levels miR-137 BCL11A cell lines. Bioinformatics analysis dual luciferase...
Enantio- and diastereoselective synthesis of multifunctional spiropyrazolone scaffolds has been achieved using secondary amine-catalyzed [4 + 2] annulations α,β,γ,δ-unsaturated pyrazolones with aldehydes. The pyrazolone substrates serve as C4 synthons to produce 6-membered, carbocycle-based, chiral derivatives. synthesized compounds showed potent toxicity against a panel cancer cell lines. most compound 3h-induced cycle arrest macroautophagy in HCT116 colorectal cells, triggering...
As an aggressive subtype of breast cancer with a high risk recurrence, triple-negative (TNBC) lacks available treatment targets. LncRNA MIR100HG promotes cell proliferation in TNBC. However, few studies have investigated the molecular mechanism Thus, additional in-depth investigations are needed to unravel its associated regulatory mechanism.MIR100HG and miR-5590-3p expression TNBC tissue samples lines was detected by RT-qPCR. Flow cytometry, transwell, wound-healing, CCK8 colony formation...
Hepatocellular carcinoma (HCC) is one of the most fatal cancers across world. Chinese medicine has been used as adjunctive or complementary therapy for management HCC. Huachansu belongs to a class toxic steroids isolated from toad venom that important anti-cancer property. This study was aimed identify bioactive constituents and molecular targets capsules (HCSCs) treating HCC using network pharmacology analysis experimental assays. The major components HCSCs were determined ultra-performance...
N6-methyladenosine (m6A) methyltransferase METTL3 has been implicated in carcinogenesis, which may be associated the overexpression of MALAT1. However, downstream mechanics actions remain largely unknown. This study intends to probe mechanism (m6 A) and MALAT1 adriamycin resistance breast cancer. Through Bioinformatics databases lncMAP, TCGA GTEx, we predicted transcription factors E2F1 AGR2 The Cancer Genome Atlas Genotype-Tissue Expression (GTEx) were used screen target genes MeRIP-qPCR...
Doxorubicin (DOX) resistance in breast cancer (BC) poses a huge challenge for the therapeutic effect on BC. Lnc KCNQ1OT1 play crucial roles chemotherapy resistance. However, role and mechanism of lnc DOX BC have not been investigated, which merits further exploration. Based MCF‐7 MDA-MB-231 cells, MCF‐7/DOX MDA-MB-231/DOX cells were established using gradient concentrations DOX. IC50 values cell viability determined MTT. Cell proliferation was investigated by colony formation. Flow cytometry...
Interleukin (IL)‑32 is a newly identified cytokine in humans and primates. It has been established that IL‑32 may antagonize cancer growth. However, to the best of our knowledge, direct effect on breast cell growth not yet investigated. In addition, rodents lack expression IL‑32; hence, effects xenografts nude mice have studied. The present study aimed examine potential regulatory cells mice. IL-32 tumor cuture xenograft model were investigated, as well apoptosis. proliferation apoptosis...
Triple-negative breast cancer (TNBC) is a highly invasive subtype of cancer. This study investigated the molecular mechanism and influences MIR503HG, miR-224-5p, homeobox A9 (HOXA9) on TNBC cell growth migration. Dual-luciferase reporter gene RNA immunoprecipitation were performed to examine regulation HOXA9. Cell proliferation, apoptosis, migration, invasion evaluated by colony formation, flow cytometry, Transwell assays. Finally, nude mice employed investigate influence MIR503HG tumor...
Our aim was to demonstrate the significance of miRNA, lncRNA, and circRNA in transformation human bronchial epithelial cells induced by benzo(a)pyrene (BaP), investigate their regulatory networks. Hierarchical clustering, gene ontology (GO) analysis, Kyoto Encyclopedia Genes Genomes (KEGG) analysis network regulation were used analyze high-throughput sequencing results cell line BEAS-2B BaP-transformed (BEAS-2B-T). 76,191,786 3,431differentially-expressed mRNA detected, respectively; 43 48...
Abstract Solid tumors are complex ecosystems, and heterogeneity is the major challenge for overcoming tumor relapse metastasis. Uncovering spatial of cell types functional states in essential developing effective treatment, especially invasive fronts tumor, most active region cells infiltration invasion. We firstly used SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq) with a nanoscale resolution to characterize microenvironment intrahepatic cholangiocarcinoma (ICC). Enrichment...