A5101462608- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Immune cells in cancer
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Tracheal and airway disorders
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Metabolomics and Mass Spectrometry Studies
- Cleft Lip and Palate Research
- Lung Cancer Research Studies
- Cellular Mechanics and Interactions
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Head and Neck Cancer Studies
- Hepatitis B Virus Studies
- Reconstructive Surgery and Microvascular Techniques
- Oral microbiology and periodontitis research
- interferon and immune responses
Nanjing University
2015-2024
Nanjing Medical University
2019-2024
Stomatology Hospital
2015-2024
Shanghai Stomatological Hospital
2009-2014
Pharmaceutical Biotechnology (Czechia)
2011
Stromal carcinoma-related fibroblasts (CAFs) are the main type of non-immune cells in tumor microenvironment (TME). CAFs interact with cancer to promote proliferation. Long non-coding RNAs (lncRNAs) known regulate cell growth, apoptosis and metastasis cells, but their role stromal is unclear. Using RNA sequencing, we identified a lncRNA signature during transformation from normal (NFs) oral squamous carcinoma (OSCC). We uncovered an uncharacterized lncRNA, FLJ22447, which was remarkably...
Saliva is a noninvasive biofluid that can contain metabolite signatures of oral squamous cell carcinoma (OSCC). Conductive polymer spray ionization mass spectrometry (CPSI-MS) employed to record wide range species within few seconds, making this technique appealing as point-of-care method for the early detection OSCC. samples from 373 volunteers, 124 who are healthy, have premalignant lesions, and 125 OSCC patients, were collected discovering validating dysregulated metabolites determining...
Objectives: Integrins, the coordinator of extracellular and intracellular signaling, are often found to be aberrant in tumors can reshape tumor microenvironment. Although previous studies showed that integrin beta 2 (ITGB2) is important for host defense, its expression profile role tumors, especially cancer associated fibroblasts (CAFs) still unknown. Methods: Immunofluorescence stain fluorescence activated cell sorting were used analyze ITGB2 oral squamous carcinoma (OSCC). RT-PCR western...
Local resident normal fibroblasts (NFs) are the major source of cancer-associated (CAFs), which distinguishable from NFs by their tumor-supportive properties. However, mechanism and effects underlying transition to CAFs in oral squamous cell carcinoma (OSCC) remain unclear. Five pairs matching primary derived OSCC patients were sent for RNA sequencing. Epiregulin (EREG) expression was analyzed IHC patients. The role EREG NF-CAF consequential on progression examined...
CD68 has been widely used as a pan-macrophage marker for tumor-associated macrophages (TAM) which always involve in carcinogenesis. But the correlations between CD68(+) TAMs and prognosis of patients show to be inconsistent, might due lack specific markers TAMs. We here found that microlocalization also played unique role with oral squamous cell carcinoma (OSCC). were identified paraffin-embedded OSCC specimens (n = 91) by using immunohistochemistry. The number was remarkably increased from...
// Shengwei Han 1, 2 , Wenguang Xu Zhiyong Wang Xiaofeng Qi Yufeng Yanhong Ni Hao Shen Qingang Hu Wei 1 Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School University, Nanjing, P.R. China Central Laboratory Stomatology, Correspondence to: Han, email: doctorhanwei@hotmail.com Hu, qghu@nju.edu.cn Keywords: oral squamous cell carcinoma, HIF-1, TLR, NF-κB, tumor microenvironment Received: November 19, 2015 Accepted:...
Abstract The traditional maximum dose density chemotherapy renders the tumor patients not only remission but resistance and more adverse side effects. According to widely positive expression of Toll-like receptor (TLR)-3 in oral squamous cell carcinoma (OSCC) (n = 166), we here provided an alternative strategy involved orderly treatment TLR3 agonist polyinosine–polycytidylic acid (PIC) low-dose cisplatin. optimal cisplatin, novel role PIC effects combined were determined vitro distinct human...
The weakened tumour colonization of attenuated Salmonella has severely hampered its clinical development.In this study, we investigated whether an anti-inflammation and antiangiogenesis compound triptolide could improve the efficacy VNP20009, a highly strain, against mice melanoma.By comparing effects conventional VNP20009 monotherapy combination therapy that uses both found significantly improved by reducing number infiltrated neutrophils in melanoma, which led to larger necrotic area...
Gelsolin (GSN) is one of the most abundant actin-binding proteins, and involved in several pathological processes, including Alzheimer's disease, cardiac injury cancer. The aim present study was to assess effect GSN on growth motility oral squamous cell carcinoma Tca8113 cells. overexpression vector pcDNA3.1-GSN transfected into cells stable line identified based G418 antibiotic selection. proliferation, apoptosis, migration invasion examined using a counting kit-8 assay, flow cytometry...
Abstract Orthodontic force-induced osteogenic differentiation and bone formation at tension side play a pivotal role in orthodontic tooth movement (OTM). Platelet-derived growth factor-BB (PDGF-BB) is clinically proven factor during regeneration process with unclear mechanisms. Fibroblasts periodontal ligament (PDL) are considered to be mechanosensitive under force. Thus, we established OTM model investigate the correlation between PDGF-BB fibroblasts side. We confirmed that tensile force...
Abstract The Pattern Of Invasion (POI) of tumor cells into adjacent normal tissues clinically predicts postoperative metastasis/recurrence early oral squamous cell carcinoma (OSCC), but the mechanisms underlying development these subtypes remain unclear. Focusing on highest score POIs (Worst POI, WPOI) present within each tumor, we observe a disease progression-driven shift WPOI towards high-risk type 4/5, associated with mesenchymal phenotype in advanced OSCC. 4-5-derived cancer-associated...
Attenuated Salmonella typhimurium ( S. ) strains can selectively grow and express exogenous genes in tumors for targeted therapy. We engineered strain VNP20009 to secrete tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) under the control of a hypoxia‐induced nirB promoter examined efficacy Salmonella‐ mediated expression TRAIL mice bearing melanoma TRAIL‐resistant RM‐1 tumor. found that VNP preferentially accumulated tissues effectively drove TRAIL. Compared with recombinant...
Acceptor–donor–acceptor structured ITIC small molecule based nanoparticles are explored for highly efficient photothermal therapy application.
The purpose of this study was to design silver nanoparticles (AgNPs) poloxamer thermoreversible gel (AgNPs-PL) and investigate whether could provide sustained antibacterial activity against Enterococcus faecalis (E. faecalis) in the root canal. gels fabricated were characterized terms gelatin temperature, particle size, in-vitro Ag+ release, elemental content. Cytotoxicity AgNPs-PL on primary human periodontal ligament fibroblasts (HPDLFs) examined by CCK-8 assay. Characterization revealed...
Purpose It is essential to explore potential molecular involved in oral squamous cell carcinoma (OSCC) malignant transformation and progression. The purpose of this study was delineate the amino acids metabolic characteristics OSCC patients test their diagnostic value. Experimental Design Eight pairs matched tumor normal samples were collected for Gas chromatography-mass spectrometry (GC-MS) high throughput untargeted analysis. Another 20 cases (each case including Tumor Normal tissues) also...
The growth of tumor is angiogenesis-dependent and it often contains hypoxia necrotic areas. Salmonella VNP20009 could target replicate in areas within induce antitumor effect. Angiogenesis inhibitor endostatin reduce angiogenesis inhibit its growth. However, the phase I trials at maximum-tolerated dose, no effects for bacteria therapy minor therapeutic treatment were seen. ineffectiveness these agents clinical suggests that combination with synergic modalities might be necessary. Here we...
Tumor-targeting bacteria have been developed as powerful anticancer agents. Salmonella typhimurium VNP20009, a representative tumor-targeting strain, has systemically administered single-agent therapy at doses of 1 x 10(6) to 3 colony-forming unit (cfu)/mouse, or in combination with other antitumor agents 10(4) 2 10(5) cfu/mouse. Recently, we reported that oral delivery VNP20009 the dose 10(9) cfu/mouse induced significant effects comparable by systemic administration this strain To further...
Solid tumors often contain hypoxic and necrotic areas that can be targeted by attenuated Salmonella typhimurium VNP20009 (VNP). We sought to develop a hypoxia- inducible promoter system based on the tumor-specific delivered strain VNP confine expression of therapeutic gene specifically or selectively within tumor microenvironment. A hypoxia-inducible - adhE was screened from hypoxia-regulated endogenous proteins through two-dimensional gel electrophoresis matrix-assisted laser desorption...
Monocyte chemotactic protein-1 (MCP-1/CCL2) and macrophage inflammatory protein-1α (MIP-1α/CCL3) are small proteins that have been found in several kinds of tumor tissue samples function as key regulators cancer progression. However, the expression CCL2 CCL3 serum oral squamous cell carcinoma (OSCC) patients remains unknown. This study aimed to investigate prognostic meaning OSCC. The concentration was assessed by ELISA OSCC (n = 98), leukoplakia 14), healthy donors 27). results showed group...
Toll-like receptor (TLR)-7 agonists have been used in cancer immunotherapy, but tumour heterogeneity means that TLR-7 activity is variable different components of the microenvironment and characteristics oral squamous cell carcinoma (OSCC) are unclear.Twenty healthy tissues, 50 leukoplakia tissues 166 retrospective primary OSCC samples were collected for immunohistochemical staining showed up-regulated expression during carcinogenesis. Moreover, patients with high cells (TCs) had poor...