A5049719465- Photoreceptor and optogenetics research
- Ion Channels and Receptors
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Metal complexes synthesis and properties
- Molecular Communication and Nanonetworks
- Nicotinic Acetylcholine Receptors Study
- Trace Elements in Health
- RNA modifications and cancer
- Drug Transport and Resistance Mechanisms
- Phytochemicals and Antioxidant Activities
- RNA Research and Splicing
- Neuroscience and Neural Engineering
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Insect Pest Control Strategies
- Nanocluster Synthesis and Applications
- Plant Stress Responses and Tolerance
- Photochromic and Fluorescence Chemistry
- Molecular Sensors and Ion Detection
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Microtubule and mitosis dynamics
Texas A&M University
2015-2024
The University of Texas MD Anderson Cancer Center
2024
Texas A&M Health Science Center
2015-2022
Mitchell Institute
2017-2019
University of Science and Technology of China
2010-2017
Cancer Research Institute
2017
High Magnetic Field Laboratory
2015-2017
In-Q-Tel
2013
The application of current channelrhodopsin-based optogenetic tools is limited by the lack strict ion selectivity and inability to extend spectra sensitivity into near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable platform (termed 'Opto-CRAC') that selectively remotely controls Ca2+ oscillations Ca2+-responsive gene expression regulate function non-excitable cells, including T lymphocytes, macrophages dendritic cells. When coupled upconversion nanoparticles,...
Widerstand ist zwecklos: Ein Platin(IV)-Prodrug, das an ein Wirkstofftransportsystem auf der Basis von Goldnanostäbchen konjugiert ist, vermeidet die Art Wirkstoffresistenz, mit Cisplatin assoziiert (siehe Bild). Das Konjugat wird durch Endocytose in Zellen aufgenommen und umgeht dadurch resistenzassoziierte Aufnahme, Kupfertransportprotein Ctr1 vermittelt wird. Platin(IV)-Prodrug gegen deaktivierendes Glutathion Metallothionein inerter als Cisplatin. Detailed facts of importance to...
Abstract Store-operated Ca 2+ entry mediated by STIM1 and ORAI1 constitutes one of the major routes in mammalian cells. The molecular choreography STIM1–ORAI1 coupling is initiated endoplasmic reticulum (ER) store depletion with subsequent oligomerization ER-luminal domain, followed its redistribution towards plasma membrane to gate channels. mechanistic underpinnings this inside-out signalling were largely undefined. By taking advantage a unique gain-of-function mutation within...
Cancer cells adopt various modes of migration during metastasis. How the ubiquitination machinery contributes to cancer cell motility remains underexplored. Here, we report that tripartite motif (TRIM) 59 is frequently up-regulated in metastatic breast cancer, which correlated with advanced clinical stages and reduced survival among patients. TRIM59 knockdown (KD) promoted apoptosis inhibited tumor growth, while overexpression led opposite effects. Importantly, uncovered as a key regulator...
Genetically encoded photoswitches have enabled spatial and temporal control of cellular events to achieve tailored functions in living cells, but their applications probe the structure-function relations signaling proteins are still underexplored. We illustrate herein incorporation various blue light-responsive photoreceptors into modular domains stromal interaction molecule 1 (STIM1) manipulate protein activity faithfully recapitulate STIM1-mediated events. Capitalizing on these optogenetic...
Abstract Stromal interaction molecule 1 (STIM1) monitors ER-luminal Ca 2+ levels to maintain cellular balance and support signalling. The prevailing view has been that STIM1 senses reduced ER through dissociation of bound from a single EF-hand site, which triggers dramatic loss secondary structure dimerization the luminal domain. Here we find domain 5–6 -binding sites, binding at these sites is energetically coupled controls switch second structured conformation rather than protein...
Abstract Platinum phenanthroline complexes inhibit amyloid‐β (Aβ) aggregation and reduce Aβ‐caused neurotoxicity [ Proc. Natl. Acad. Sci. , 2008 105 6813–6818]. In this study, we investigated the interactions of Aβ 1–16 with [PtCl 2 (phen)] (phen=1,10‐phenanthroline) using HPLC, ESI‐MS, NMR spectroscopy characterized identity products tandem mass spectrometry. Results indicated that ligand could induce noncovalent between peptide platinum complexes, leading to rapid platination. Multiple...
A light-controllable membrane tether to control protein subcellular localization and manipulate interorganellar contact sites at nanoscales.
The Ca2+ release-activated (CRAC) channels control many Ca2+-modulated physiological processes in mammals. Hyperactivating CRAC are known to cause several human diseases, including Stormorken syndrome. Here, we show the design of azopyrazole-derived photoswitchable channel inhibitors (designated piCRACs), which enable optical inhibition store-operated influx and downstream signaling. Moreover, piCRAC-1 has been applied vivo alleviate thrombocytopenia hemorrhage a zebrafish model syndrome...
The current optogenetic toolkit lacks a robust single-component Ca2+-selective ion channel tailored for remote control of Ca2+ signaling in mammals. Existing tools are either derived from engineered channelrhodopsin variants without strict selectivity or based on the stromal interaction molecule 1 (STIM1) that might crosstalk with other targets. Here, we describe design light-operated (designated LOCa) by inserting plant-derived photosensory module into intracellular loop an ORAI1 channel....
Mediated through the combined action of STIM proteins and Orai channels, store-operated Ca2+ entry (SOCE) functions ubiquitously among different cell types. The existence multiple genes has made it difficult to assign specific roles each homolog in mediating signals. Using CRISPR/Cas9 gene editing tools, we generated cells with both or all three homologs deleted directly monitored store We found that unstimulated, SOCE null KO still retain 50~70% ER stores wildtype (wt) cells. After brief...
The endoplasmic reticulum (ER) Ca2+ sensors stromal interaction molecule 1 (STIM1) and STIM2, which connect ER depletion with extracellular influx, are crucial for the maintenance of homeostasis in mammalian cells. Despite recent progress unraveling role STIM2 signaling, mechanistic underpinnings its activation remain underexplored. We use an engineering approach to direct ER-resident STIMs plasma membrane (PM) while maintaining their correct topology, as well Förster resonance energy...
Platinum phenanthroline complexes have been found to inhibit Aβ aggregation and reduce caused neurotoxicity. Our previous results revealed the synergistic roles of ligand Pt(ii) coordination in inhibition aggregation. In this work, reactions PtCl2(phen) with metal bound were investigated. HPLC show that copper decreases reaction rate Aβ1-16 influences distribution products on profiles. EPR reveal Cu(2+) remains coordinated peptide upon binding [Pt(phen)](2+), however, sites are changed. The...
Abstract Voltage‐gated Ca 2+ (Ca V ) channels mediate entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering RGK GTPases, class genetically encoded channel modulators, enable photo‐tunable modulation activity in mammalian cells. This optogenetic tool (designated optoRGK) tailored for could find broad applications interrogating wide range ‐mediated physiological processes.
Methionine binding converts monofunctional platinum antitumor agents to trans configuration adducts in the reaction with DNA, which suggests correlation of two classes complexes mechanism.