A5101855504- RNA modifications and cancer
- Cancer-related gene regulation
- RNA Research and Splicing
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Toxic Organic Pollutants Impact
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Effects and risks of endocrine disrupting chemicals
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Zeolite Catalysis and Synthesis
- Carcinogens and Genotoxicity Assessment
- Advanced Sensor and Energy Harvesting Materials
- Renal and related cancers
- Plant-Microbe Interactions and Immunity
- Adhesion, Friction, and Surface Interactions
- Mitochondrial Function and Pathology
- Metal-Organic Frameworks: Synthesis and Applications
- Cancer and Skin Lesions
- Hair Growth and Disorders
- Polymer Surface Interaction Studies
- Geochemistry and Elemental Analysis
Center for Excellence in Molecular Cell Science
2017-2025
University of Chinese Academy of Sciences
2017-2025
Chinese Academy of Sciences
2004-2025
Research Center for Eco-Environmental Sciences
2024-2025
Air Force Medical University
2024-2025
Xijing Hospital
2024-2025
Southern Medical University
2023-2024
Nanfang Hospital
2023-2024
Wuhan Donghu University
2024
Wuhan University
2024
A systematic interrogation of male germ cells is key to complete understanding molecular mechanisms governing spermatogenesis and the development new strategies for infertility therapies contraception. Here we develop an approach purify all types homogeneous spermatogenic by combining transgenic labeling synchronization cycle seminiferous epithelium, subsequent single-cell RNA-sequencing. We reveal extensive previously uncharacterized dynamic processes signatures in gene expression, as well...
Abstract Osmoregulation is important for plant growth, development and response to environmental changes. SNF1-related protein kinase 2s (SnRK2s) are quickly activated by osmotic stress central components in abscisic acid (ABA) signaling pathways; however, the upstream required SnRK2 activation early still unknown. Here, we report a critical role B2, B3 B4 subfamilies of Raf-like kinases (RAFs) as well ABA Arabidopsis thaliana . RAFs phosphorylation SnRK2s. Analyses high-order mutants reveal...
Abstract The phytohormone abscisic acid (ABA) is crucial for plant responses to environmental challenges. SNF1-regulated protein kinase 2s (SnRK2s) are key components in ABA-receptor coupled core signaling, and rapidly phosphorylated activated by ABA. Recent studies have suggested that Raf-like kinases (RAFs) participate ABA-triggered SnRK2 activation. In vitro assays also suggest the existence of autophosphorylation SnRK2s. Thus, how quickly during ABA signaling still needs be clarified....
Abstract Proper follicle development is very important for the production of mature oocytes, which essential maintenance female fertility. This complex biological process requires precise gene regulation. The most abundant modification mRNA, N 6 -methyladenosine (m A), involved in many RNA metabolism processes, including splicing, translation, stability, and degradation. Here, we report that m A plays roles during oocyte development. Oocyte-specific inactivation key methyltransferase Mettl3...
Abstract Background Hair follicle stem cells (HFSCs) typically remain quiescent and are activated only during the transition from telogen to anagen ensure that hair enters a new cycle. The metabolic behavior of in tissues is regulated by macroautophagy/autophagy, changes HFSC metabolism directly affect their activation maintenance. However, role autophagy regulation function remains unclear. Methods Back skin samples were obtained mice at different cycle stages, immunofluorescence staining...
ASH2L is a core component of KMT2 complexes, crucial for H3K4 trimethylation. However, its role in spermatogenesis remains elusive. Here, we demonstrate an essential Ash2l meiotic prophase but dispensable mitosis differentiated spermatogonia. Using germ cell-specific knockout mouse model, reveal that deficiency leads to arrest and sterility both sexes. Ash2l-deficient spermatocytes exhibit failures chromosomal synapsis associated with persistent DMC1 foci γH2AX, resulting meiocyte loss due...
Abstract Epigenetic programming governs cell fate determination during development through intricately controlling sequential gene activation and repression. Although H3K4me3 is widely recognized as a hallmark of activation, its role in modulating transcription output timing within continuously developing system remains poorly understood. In this study, we provide detailed characterization the epigenomic landscapes male germ cells. We identified thousands spermatid-specific broad domains...
The present study provides information on the time trend of PBDEs in three sediment cores from Pearl River Estuary (PRE), South China, using 210Pb dating technique. ΣPBDEs (except for BDE 209) concentrations all increased gradually bottom (mid-1970s) to middle layer (later 1980s and early 1990s) followed by different temporal trends locations surface sediments, reflecting variations consumption commercial penta-BDEs mixture regions Delta. 209 remained constant until 1990 thereafter...
The N6-methyladenosine (m6A) RNA modification is essential during embryonic development of various organs. However, its role in and early postnatal islet remains unknown. Mice which methyltransferase-like 3/14 (Mettl3/14) were deleted Ngn3+ endocrine progenitors (Mettl3/14nKO) developed hyperglycemia hypoinsulinemia at 2 weeks after birth. We found that Mettl3/14 specifically regulated both functional maturation mass expansion neonatal β-cells before weaning. Transcriptome m6A methylome...
Abstract Meiotic recombination is initiated by the formation of double-strand breaks (DSBs), which are repaired as either crossovers (COs) or noncrossovers (NCOs). In most mammals, PRDM9-mediated H3K4me3 controls nonrandom distribution DSBs; however, both timing and mechanism DSB fate control remain largely undetermined. Here, we generated comprehensive epigenomic profiles synchronized mouse spermatogenic cells during meiotic prophase I, revealing spatiotemporal functional relationships...
BACKGROUND: Myocardial mitochondrial dysfunction underpins the pathogenesis of heart failure (HF), yet therapeutic options to restore myocardial function are scarce. Epigenetic modifications DNA (mtDNA), such as methylation, play a pivotal role in modulating homeostasis. However, their involvement HF remains unclear. METHODS: Experimental models were established through continuous angiotensin II and phenylephrine (AngII/PE) infusion or prolonged ischemia/reperfusion injury. The landscape N 6...
Reversible adhesives are widely needed in our daily lives and industrial applications. However, robust switchable adhesion on rough surfaces with on-demand attachment detachment remains highly challenging. Here, we report a snail-mucus-inspired touch-responsive hydrogel (TRH), whose universal is triggered by simple contact the attaching surface. TRH composed of polymeric saturated sodium acetate (NaAc) prepared one-pot synthesis. At room temperature, an amorphous soft state, which allows it...
Abstract Hydrothermal reactions of InCl 3 with pyromellitic dianhydride and pyridine derivatives produce three coordination polymers: {(HL)[In (btec) 2 (OH) ] · L} n [btec = 1,2,4,5‐benzenetetracarboxylate, L 2‐picoline ( 1 ), 4‐picoline )] {(Hdpea)[In ]} [dpea 1,2‐bis(4‐pyridyl)ethane] ). Single crystal X‐ray diffraction powder analyses reveal that compounds – are isostructural possess the same 3D framework as 1D open channels. Thermal gravimetric studies show stable up to 300 °C. The...
Sertoli cells are the major component of spermatogonial stem cell (SSC) niche; however, regulatory mechanisms in that dictate SSC fate decisions remain largely unknown. Here we revealed features N6-methyladenosine (m6A) mRNA modification and demonstrated functions WTAP, key subunit m6A methyltransferase complex spermatogenesis. m6A-sequencing analysis identified 21,909 sites from 15,365 putative m6A-enriched transcripts within 6,122 genes, including many cell-specific genes. Conditional...
Inspired by the adhesive capabilities of snails, this work successfully develops a touch-initiated hydrogel that exhibits remarkable on-demand adhesion on diverse range surfaces.
Nuclear RNA decay has emerged as a mechanism for post-transcriptional gene regulation in cultured cells. However, whether this process occurs animals and holds biological relevance remains largely unexplored. Here, we demonstrate that MTR4, the central cofactor of nuclear exosome, is essential embryogenesis spermatogenesis. Embryonic development Mtr4 knockout mice arrests at 6.5 day. Germ cell-specific results male infertility with specific severe defect meiotic initiation. During...
BACKGROUND: Heart failure with preserved ejection fraction (HFpEF) has become the most prevalent type of heart failure, but effective treatments are lacking. Cardiac lymphatics play a crucial role in maintaining health by draining fluids and immune cells. However, their involvement HFpEF remains largely unexplored. METHODS: We examined cardiac lymphatic alterations mice comorbid obesity hypertension, tissues from patients HFpEF. Using genetically engineered mouse models various cellular...
The R-loop is a naturally formed three-strand nucleic acid structure that recently has been reported to participate in multiple biological processes and helped answer some previously unexplained scientific questions. Meiosis process involves chromatin-related events such as DNA double-stranded breaks (DSB) formation, repairing transcriptional dynamics.Explore the regulatory roles physiological functions of R-loops mammalian meiosis process.In our study, using genome-wide S9.6 CUT & Tag seq,...
ABSTRACT The most significant feature of meiosis is the recombination process during prophase I. CXXC finger protein 1 (CXXC1) binds to CpG islands and mediates deposition H3K4me3 by SETD1 complex. CXXC1 also predicted recruit H3K4me3-marked regions chromosome axis for generation double-strand breaks (DSBs) in meiosis. Therefore, we deleted Cxxc1 before onset with Stra8-Cre. conditional knockout mice were completely sterile spermatogenesis arrested at MII. Knockout led a decrease level from...