Naijing Hu

ORCID: 0000-0003-2659-6837
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About
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Research Areas
  • SARS-CoV-2 and COVID-19 Research
  • RNA Research and Splicing
  • RNA modifications and cancer
  • Bacteriophages and microbial interactions
  • Monoclonal and Polyclonal Antibodies Research
  • RNA and protein synthesis mechanisms
  • Immunotherapy and Immune Responses
  • Viral gastroenteritis research and epidemiology
  • Phagocytosis and Immune Regulation
  • Erythrocyte Function and Pathophysiology
  • Cancer-related molecular mechanisms research
  • Genomics and Chromatin Dynamics
  • CAR-T cell therapy research
  • Glycosylation and Glycoproteins Research
  • Cancer Immunotherapy and Biomarkers
  • Streptococcal Infections and Treatments
  • Poxvirus research and outbreaks
  • Bacillus and Francisella bacterial research
  • Viral Infections and Immunology Research
  • Toxin Mechanisms and Immunotoxins
  • Antimicrobial Resistance in Staphylococcus
  • DNA Repair Mechanisms
  • Botulinum Toxin and Related Neurological Disorders
  • Natural product bioactivities and synthesis
  • Galectins and Cancer Biology

Chinese Academy of Sciences
2020-2025

Institute of Biophysics
2020-2025

Academy of Military Medical Sciences
2025

Institute of Pharmacology
2021-2025

University of Chinese Academy of Sciences
2021-2025

Henan University
2022

Dali University
2022

Chinese PLA General Hospital
2021-2022

Hunan Normal University
2022

Shenyang Pharmaceutical University
2021

Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we develop a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstruct tertiary reveal surprisingly “unentangled globule” conformation. We uncover many long-range duplexes higher-order junctions,...

10.1038/s41467-021-22785-x article EN cc-by Nature Communications 2021-06-24

A series of novel linear aliphatic amine-linked triaryl derivatives as inhibitors PD-1/PD-L1 were designed, synthesized, and evaluated in vitro vivo. In this chemical series, compound 58 showed the most potent inhibitory activity binding affinity with hPD-L1, an IC50 value 12 nM a KD 16.2 pM, showing potency approximately 2000-fold that hPD-1. Compound could bind hPD-L1 on cellular surface competitively block interaction hPD-1 hPD-L1. T cell function assay, restored function, leading to...

10.1021/acs.jmedchem.0c01329 article EN Journal of Medicinal Chemistry 2020-11-13

Botulinum neurotoxin (BoNT) is a highly lethal toxin produced by the anaerobic bacterium Clostridium botulinum, which leads to nerve paralysis following poisoning. At present, there no specific drug officially approved. Antibodies, particularly single-domain antibodies, represent safe and effective candidates for drugs against BoNT. In this study, receptor-binding domain of botulinum (BoNT/AHCC) was utilized immunize Bactrian camels, resulting in generation nanobody phage library. From...

10.1016/j.heliyon.2025.e42616 article EN cc-by-nc-nd Heliyon 2025-02-01

Nuclear RNA decay has emerged as a mechanism for post-transcriptional gene regulation in cultured cells. However, whether this process occurs animals and holds biological relevance remains largely unexplored. Here, we demonstrate that MTR4, the central cofactor of nuclear exosome, is essential embryogenesis spermatogenesis. Embryonic development Mtr4 knockout mice arrests at 6.5 day. Germ cell-specific results male infertility with specific severe defect meiotic initiation. During...

10.1038/s41467-025-57898-0 article EN cc-by-nc-nd Nature Communications 2025-03-17

The continuous mutation of SARS-CoV-2 has presented enormous challenges to global pandemic prevention and control. Recent studies have shown evidence that the genome sequence nucleocapsid proteins is relatively conserved, their biological functions are being confirmed. There increasing N protein will not only provide a specific diagnostic marker but also become an effective treatment target. In this study, 2G4, which specifically recognizes protein, was identified by screening human phage...

10.1038/s41598-022-12242-0 article EN cc-by Scientific Reports 2022-05-19

Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we developed a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstructed tertiary revealed surprisingly "unentangled globule" conformation. We uncovered many long-range duplexes higher-order...

10.21203/rs.3.rs-132578/v1 preprint EN cc-by Research Square (Research Square) 2020-12-22

Phagocytic resistance plays a key role in tumor-mediated immune escape, so phagocytosis checkpoints are potential target for cancer immunotherapy. CD47 is one of the important checkpoints; thus, blocking interaction between and signal regulatory protein <i>α</i> (SIRP<i>α</i>) may provide new options treatment. Using computer-aided targeted epitope mammalian cell-displayed antibody library, we screened obtained an engineered SIRP<i>α</i> variant fragment crystallizable fusion protein, FD164,...

10.1124/molpharm.120.000202 article EN Molecular Pharmacology 2021-07-27

Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we developed a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstructed tertiary revealed surprisingly "unentangled globule" conformation. We uncovered many long-range duplexes higher-order...

10.21203/rs.3.rs-132578/v2 preprint EN cc-by Research Square (Research Square) 2021-03-11

Sialic acid-binding Ig-like lectin-15 is an important immunosuppressive molecule considered to be a key target in next-generation tumor immunotherapy. In this study, we screened 22 high-affinity antibodies that specifically recognize human Siglec-15 by using large phage antibody library, and five representative sequences were selected for further study. The results showed the binding activity of (EC<sub>50</sub> ranged from 0.02368 μg/mL 0.07949 μg/mL), two Siglec-15-overexpressed cell...

10.1124/molpharm.121.000470 article EN Molecular Pharmacology 2022-06-28

CD47, as an innate immune checkpoint molecule, is important target of cancer immunotherapy. We previously reported that a high-affinity SIRPα variant FD164 fused with IgG1 subtype Fc showed better antitumor effect than wild-type in immunodeficient tumor-bearing model. However, CD47 widely expressed blood cells, and the drugs targeting may cause potential hematological toxicity. Herein, we modified molecule by mutation (N297A) to inactivate Fc-related effector function named it nFD164....

10.1016/j.biopha.2023.114618 article EN cc-by-nc-nd Biomedicine & Pharmacotherapy 2023-04-01

SUMMARY RNA-binding proteins bind at different positions of pre-mRNA molecules to promote or reduce the usage a particular exon. Seeking understand working principle these positional effects, we develop CRIC-seq method enrich single RBP-mediated in situ RNA-RNA spatial interacting fragments for deep sequencing. We determine hnRNPA1- and PTBP1-mediated interactions regulatory mechanisms HeLa cells. Unexpectedly, 3D RNA map analysis shows that loops introns preferably cassette exon splicing by...

10.1101/2022.08.09.503273 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2022-08-11

Immunotherapy with anti-programmed cell death protein-1 (PD-1)/programmed ligand-1 (PD-L1) monoclonal antibodies has become routine in the treatment of many kinds human cancers, such as lung cancer, intestinal and melanoma. The PD-1/PD-L1 pathway inhibits T activation micro-environment, making it an attractive target against cancer. Wild-type (WT) PD-1 ectodomain been shown to have difficulty blocking mixture formation due its low affinity. present work uses three-dimensional (3D) crystal...

10.1016/j.eng.2020.11.011 article EN cc-by-nc-nd Engineering 2021-07-13

SARS-CoV-2 RNA interacts with host factors to suppress interferon responses and simultaneously induces cytokine release drive the development of severe coronavirus disease 2019 (COVID-19). However, how hijacks RNAs elicit such imbalanced immune remains elusive. Here, we analyzed in situ structures interactions infected cells patient lung samples using RIC-seq. We discovered that forms 973 potential duplexes 3'UTRs 142 mRNAs increase their stability by recruiting RNA-binding protein YBX3....

10.2139/ssrn.4369784 preprint EN 2023-01-01

RNA-binding proteins (RBPs) can bind and mediate RNA-RNA contacts. However, identifying specific RBP-organized contacts remains challenging. Here, we present a capture RIC-seq (CRIC-seq) technique to map RBP-associated globally. We describe steps for formaldehyde cross-linking fix RNA in situ conformation, pCp-biotin labeling mark juncture, proximity ligation join proximal RNAs. then detail immunoprecipitation isolate contacts, biotin-streptavidin selection enrich chimeric RNAs, library...

10.1016/j.xpro.2023.102401 article EN cc-by-nc-nd STAR Protocols 2023-07-04

Siglec-15, as an important immunosuppressive molecule, is considered target in the next generation of tumor immunotherapy. In this study, we screened multiple high affinity antibodies that specifically recognized human siglec-15 by using phage antibody library with large capacity, and verified their binding, blocking biological activities vitro vivo. The results showed selected could effectively bind to overexpressed cell lines. As reported ligands siglec-15, binding activity sialyl-Tn,...

10.2139/ssrn.3946270 article EN SSRN Electronic Journal 2021-01-01
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