A5016372231- Ophthalmology and Visual Impairment Studies
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Retinopathy of Prematurity Studies
- Connexins and lens biology
- Intraocular Surgery and Lenses
- Photoreceptor and optogenetics research
- Spaceflight effects on biology
- Circadian rhythm and melatonin
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Cell death mechanisms and regulation
- Visual perception and processing mechanisms
- Retinal Imaging and Analysis
- Neuropeptides and Animal Physiology
- DNA Repair Mechanisms
- Structural Engineering and Vibration Analysis
- melanin and skin pigmentation
- Adenosine and Purinergic Signaling
- Neuroscience of respiration and sleep
Cell Technology (China)
2025
Southern Medical University
2025
Macau University of Science and Technology
2025
Wenzhou Medical University
2015-2024
Affiliated Eye Hospital of Wenzhou Medical College
2015-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2024
Zhejiang Lab
2021-2024
Shanghai Jiao Tong University
2024
Shanghai Ninth People's Hospital
2024
Hubei Normal University
2024
Worldwide, myopia is the leading cause of visual impairment. It results from inappropriate extension ocular axis and concomitant declines in scleral strength thickness caused by extracellular matrix (ECM) remodeling. However, identities initiators signaling pathways that induce ECM remodeling are unknown. Here, we used single-cell RNA-sequencing to identify activated sclera during development. We found hypoxia-signaling, eIF2-signaling, mTOR-signaling were murine myopic sclera. Consistent...
Purpose: The purpose of this study was to changes in choroidal thickness (ChT) and blood perfusion (ChBP), the correlation between them, guinea pig myopia. Methods: reliability optical coherence tomography angiography (OCTA) for measuring ChT ChBP verified pigs, after cervical dislocation (n = 7) or temporal ciliary artery transection 6). Changes refraction, axial length, ChT, were measured during spontaneous myopia 9), monocular form-deprivation (FDM, n 13), lens-induced (LIM, 14), 4 days...
Purpose: To explore the association of choroidal vascularity and choriocapillaris blood perfusion with myopic severity in anisomyopes. Methods: Refractive error, axial length (AL), other biometric parameters were measured 34 anisomyopic young adults. Macular thickness (ChT) vascularity, including total area (TCA), luminal (LA), stromal (SA), index (CVI), determined from swept-source optical coherence tomography (SS-OCT) vertical horizontal B-scans. The percentage flow voids (FV%) was...
MicroRNAs (miRNAs) are endogenously expressed, noncoding, small RNAs that inhibit protein translation through binding to target mRNAs. Recent studies have demonstrated miRNAs can regulate tumor cell proliferation and migration. MicroRNA-34a (miR-34a), a potential key effector of the p53 tumor-suppressor gene, was studied as suppressor in uveal melanoma.Northern blot analysis performed detect expression level miR-34a melanoma cells melanocytes. Subsequently, migration were examined by MTS...
Purpose: In guinea pigs, choroidal thickness (ChT) and blood perfusion (ChBP) simultaneously decrease in experimental myopia, both increase during recovery. However, the causal relationship between ChBP myopia requires further investigation. this study, we examined changes of with three different antimyopia treatments. We also actively increased to examine direct effect on development pigs. Methods: Experiment 1: Guinea pigs wore occluders right eye for two weeks induce form-deprivation...
To determine whether dopamine receptor D1 (D1R) signaling pathway activation by bright light (BL) in specific retinal neuronal cell types contributes to inhibiting form-deprivation myopia (FDM) mice.Mice (3-weeks old) were raised under either normal (NL: 100-200 lux) or BL (2500-5000 conditions with without form deprivation. Refraction changes evaluated an eccentric infrared photorefractor, and ocular axial components optical coherence tomography. The D1R antagonist, SCH39166, was...
Leber's hereditary optic neuropathy (LHON) is the most common mitochondrial disorder. Nuclear modifier genes are proposed to modify phenotypic expression of LHON-associated DNA (mtDNA) mutations. By using an exome sequencing approach, we identified a LHON susceptibility allele (c.572G>T, p.191Gly>Val) in YARS2 gene encoding tyrosyl-tRNA synthetase, which interacts with m.11778G>A mutation cause visual failure. We performed functional assays by lymphoblastoid cell lines derived from members...
BackgroundMyopia is a good model for understanding the interaction between genetics and environmental stimuli. Here we dissect biological processes affecting myopia progression.MethodsHuman Genetic Analyses: (1) gene set analysis (GSA) of new genome wide association study (GWAS) data 593 individuals with high (refraction ≤ -6 diopters [D]); (2) over-representation (ORA) 196 genes de novo mutations, identified by whole sequencing 45 high-myopia trio families, (3) ORA 284 previously reported...
Purpose: The development of myopia in guinea pigs can be inhibited by attenuating scleral hypoxia increasing choroidal blood perfusion (ChBP). In this study, we reduced ChBP through surgical and pharmacological methods to determine the effect on development. We also determined whether was quinpirole, a drug that enhances form-deprivation (FDM). Methods: right eyes via transection temporal ciliary arteries or daily injections phenylephrine into inferior peribulbar space for one week during...
Myopia is a leading cause of visual impairment and blindness worldwide. However, safe accessible approach for myopia control prevention currently unavailable. Here, we investigated the therapeutic effect dietary supplements omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on progression in animal models decreases choroidal blood perfusion (ChBP) caused by near work, risk factor young adults. We demonstrated that daily gavage ω-3 PUFAs (300 mg docosahexaenoic acid [DHA] plus 60...
The development of the anterior segment mammalian eye is critical for normal ocular function, whereas abnormal can cause glaucoma, a leading blindness in world. We report that orphan G protein-coupled receptor 48 (Gpr48/LGR4) plays an important role structure. Disruption Gpr48 causes wide spectrum dysgenesis (ASD), including microphthalmia, iris hypoplasia, irdiocorneal angle malformation, cornea dysgenesis, and cataract. Detailed analyses reveal defective myogenesis extracellular matrix...
purpose. To investigating the role of mitochondrial haplotypes in development Leber's hereditary optic neuropathy (LHON) associated with ND4 G11778A mutation Chinese families. methods. A three-generation family LHON was studied by clinical and genetic evaluation as well molecular biochemical analysis (mt)DNA. results. This exhibits a high penetrance expressivity visual impairment. The average age at onset 13.9 years this family. Of members, 86% male 29% female matrilineal relatives had loss,...
Uveal melanoma is the most common primary intraocular malignancy in adult humans. Unlike cutaneous melanoma, uveal disseminates preferentially to liver through hematogenous system. To date, mechanism underlying this metastatic homing largely unknown. This study investigated effect of hepatocyte growth factor (HGF)-triggered signaling pathways identify role HGF and its downstream effectors inducing migration cells.Migration cells was measured by vitro wound healing transwell assays. The...
High-grade myopia (HM) is highly heritable, and has a high prevalence in the Han Chinese population. We carried out genome-wide association study involving 102 HM cases suffering from retinal degeneration, 335 controls who were free fundus diseases. Significant single-nucleotide polymorphisms replicated two follow-up studies: stage I involved 2628 independent 9485 controls, II further 263 586 HM-free controls. The results combined meta-analysis. Cases drawn A locus an intergenic region at...