A5101462684- Plant Stress Responses and Tolerance
- Mitochondrial Function and Pathology
- Aluminum toxicity and tolerance in plants and animals
- Heavy metals in environment
- ATP Synthase and ATPases Research
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Micronutrient Interactions and Effects
- Heavy Metals in Plants
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- Microbial bioremediation and biosurfactants
- Pesticide Residue Analysis and Safety
- Plant responses to water stress
- Analytical chemistry methods development
- RNA modifications and cancer
- Fungal and yeast genetics research
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Agricultural safety and regulations
- Plant and animal studies
- Epigenetics and DNA Methylation
- Seed Germination and Physiology
Women's Hospital, School of Medicine, Zhejiang University
2021-2025
Shanghai Academy of Agricultural Sciences
2008-2024
Zhejiang University
2008-2021
Children's Hospital of Zhejiang University
2016-2021
Ministry of Education of the People's Republic of China
2020
Institute of Genetics
2020
Hangzhou Normal University
2015
Pennsylvania State University
2013-2014
Biotechnology Research Institute
2009-2012
Zhejiang University of Science and Technology
2008-2010
Carex (Cyperaceae), with an estimated 2000 species, nearly cosmopolitan distribution and broad range of habitats, is one the largest angiosperm genera in temperate zone. In this article, we provide argument evidence for a broader circumscription to add all species currently classified Cymophyllus (monotypic), Kobresia (c. 60 species), Schoenoxiphium 15 species) Uncinia 70 those as Carex. these comprise tribe Cariceae (subfamily Cyperoideae, Cyperaceae) form well-supported monophyletic group...
Leber's hereditary optic neuropathy (LHON) is the most common mitochondrial disorder. Nuclear modifier genes are proposed to modify phenotypic expression of LHON-associated DNA (mtDNA) mutations. By using an exome sequencing approach, we identified a LHON susceptibility allele (c.572G>T, p.191Gly>Val) in YARS2 gene encoding tyrosyl-tRNA synthetase, which interacts with m.11778G>A mutation cause visual failure. We performed functional assays by lymphoblastoid cell lines derived from members...
The phytotoxicity and antioxidative adaptations of lead (Pb) accumulating ecotype (AE) non-accumulating (NAE) Sedum alfredii Hance were investigated under different Pb treatments involving 0, 0.02 mmol/L Pb, 0.1 Pb/0.1 ethylenediaminetetraacetic acid (EDTA) for 6 days. With the increasing level, concentration in shoots AE plants enhanced accordingly, EDTA supply helped 51% translocation to compared with those treated alone. Moreover, presence alleviated through changes plant biomass, root...
Plant guard cells gate CO2 uptake and transpirational water loss through stomatal pores. As a result of decades experimental investigation, there is an abundance information on the involvement specific proteins secondary messengers in regulation movements pairwise relationships between cell components. We constructed multi-level dynamic model signal transduction during light-induced opening effect plant hormone abscisic acid (ABA) this process. The integrates into coherent network direct...
Abstract Individual metabolites have been implicated in abscisic acid (ABA) signaling guard cells, but a metabolite profile of this specialized cell type is lacking. We used liquid chromatography–multiple reaction monitoring mass spectrometry for targeted analysis 85 signaling-related Arabidopsis thaliana protoplasts over time course ABA treatment. The utilized ∼350 million from ∼30,000 plants the Columbia accession (Col) wild and heterotrimeric G-protein α subunit mutant, gpa1, which has...
Leber's hereditary optic neuropathy (LHON) is a maternally inherited eye disease. X-linked nuclear modifiers were proposed to modify the phenotypic manifestation of LHON-associated mitochondrial DNA (mtDNA) mutations. By whole-exome sequencing, we identified LHON modifier (c.157C>T, p.Arg53Trp) in PRICKLE3 encoding protein linked biogenesis ATPase 3 Chinese families. All affected individuals carried both ND4 11778G>A and p.Arg53Trp mutations, while subjects bearing only single mutation...
Abstract: Phytoremediation has gained increased attention as a cost-effective method for the remediation of heavy metal-contaminated sites. Because some plants possess range potential mechanisms that may be involved in detoxification metals, they manage to survive under metal stresses. High tolerance toxicity could rely either on reduced uptake or plant internal sequestration, which is manifested by an interaction between genotype and its environment. The growing application molecular...
OsDREB1D, a special DREB (dehydration responsive element binding protein) homologous gene, whose transcripts cannot be detected in rice (Oryza sativa L), either with or without stress treatments, was amplified from the genome DNA. The yeast one-hybrid assay revealed that OsDREB1D able to form complex dehydration element/C-repeat motif. It can also bind sequence of LTRE (low temperature element). To analyze function gene transformed and over-expressed Arabidopsis thaliana cv. Columbia....
During embryo implantation, blastocyst interacts with the receptivity endometrium and endometrial epithelium secretes nurturing fluid to support embryonic development. Interferon-λ (IFN-λ) is a novel, non-redundant regulator that participates in fetal-maternal interaction; however, precise molecular mechanism underlying its impact on uterine remains elusive. Here, microarray profiling revealed 149 specific miRNAs were differentially expressed human cells following IFN-λ treatment. In...
In this report, we investigated the pathogenic mechanism underlying deafness-associated mitochondrial(mt) tRNAAsp 7551A > G mutation. The m.7551A mutation is localized at a highly conserved nucleotide(A37), adjacent (3') to anticodon, which important for fidelity of codon recognition and stabilization in functional tRNAs. It was anticipated that altered structure function mt-tRNAAsp primer extension assay demonstrated created m1G37 modification Using cybrid cell lines generated by...
Mitochondria maintain a distinct pool of ribosomal machinery, including tRNAs and activating enzymes, such as mitochondrial tyrosyl-tRNA synthetase (YARS2). Mutations in YARS2, which typically lead to the impairment protein synthesis, have been linked an array human diseases optic neuropathy. However, lack YARS2 mutation animal model makes us difficult elucidate pathophysiology underlying deficiency. To explore this system, we generated knockout (KO) HeLa cells zebrafish using CRISPR/Cas9...
Sedum alfredii Hance has been reported to be a Zn-hyperaccumulator plant species. In this study, root morphological and physiological response of the hyperaccumulating ecotype S. H. (HE) from mined area non-hyperaccumulating (NHE) agricultural supplied levels Zn Pb were investigated. The results showed that concentrations in leaves stems HE 34 41 times higher, whereas lead 1.9 2.4 greater, respectively, than those NHE when grown at 1224 microM and/or 200 Pb. At combined supply with Pb,...
Hydroponics studies were conducted to investigate the antioxidant adaptations, ascorbate and glutathione metabolism in hyperaccumulating ecotype of Sedum alfredii (HE) exposed high Cd environment, when compared with its nonhyperaccumulating (NHE). Exposure induced a burst oxidative stress both ecotypes which was evident by sharp increase hydrogen peroxide (H(2)O(2)) contents lipid peroxidation. Buthionine sulfoximine (BSO), an inhibitor (GSH) synthesis, did not affect H(2)O(2) concentrations...
Abstract Mitochondrial DNA (mtDNA) mutations have been associated with Leber’s hereditary optic neuropathy (LHON) and their pathophysiology remains poorly understood. In this study, we investigated the of a LHON susceptibility allele (m.3394T>C, p.30Y>H) in (MT)-ND1 gene. The incidence m.3394T>C mutation was 2.7% cohort 1741 probands LHON. Extremely low penetrances were observed 26 pedigrees carrying only mutation, while 21 families bearing m.3394T>C, together...
Abstract: Cyclin-Dependent Kinase 8 (CDK8) is a paracrine transcriptional regulator involved in regulating cellular stress response, growth, and neurological functions conjunction with mediator complex subunits 12 (MED12), MED13, cyclin C. Studying the relationship be-tween CDK8 cervical squamous cell carcinoma (CESC) has significant clinical implications diagnosis, treatment, prognosis. Therefore, we analyzed between poor prognosis of CESC. The results indicated that was overexpressed CESC,...