A5016540627- Mitochondrial Function and Pathology
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Cancer-related molecular mechanisms research
- Helminth infection and control
- Coccidia and coccidiosis research
- Nematode management and characterization studies
- Hearing, Cochlea, Tinnitus, Genetics
- Microbial infections and disease research
- Animal Virus Infections Studies
- Studies on Chitinases and Chitosanases
- Virus-based gene therapy research
- Synthesis and Catalytic Reactions
- Catalytic C–H Functionalization Methods
- Colorectal Cancer Screening and Detection
- Viral Infections and Immunology Research
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Vestibular and auditory disorders
- Parasitic infections in humans and animals
- Sperm and Testicular Function
- Global Cancer Incidence and Screening
- Parasites and Host Interactions
- Antifungal resistance and susceptibility
Shangqiu First People's Hospital
2025
Taizhou University
2017-2024
The University of Texas Health Science Center at San Antonio
2024
Taizhou Central Hospital
2024
Hebei Medical University
2024
Shihezi University
2015-2022
Zhejiang University
2013-2020
Xinjiang Production and Construction Corps
2015-2019
China Pharmaceutical University
2017
McGill University Health Centre
2011-2012
In this report, we investigated the molecular genetic mechanism underlying deafness-associated mitochondrial tRNAHis 12201T>C mutation. The destabilization of a highly conserved base-pairing (5A-68U) by m.12201T>C mutation alters structure and function tRNAHis. Using cybrids constructed transferring mitochondria from lymphoblastoid cell lines derived Chinese family into mtDNA-less (ρo) cells, showed ∼70% decrease in steady-state level mutant cybrids, compared with control cybrids. changed...
Abstract: Seminal oxidative stress occurs when there is an increased production of reactive oxygen species (ROS) and/or a decrease antioxidant activity, promoting impaired sperm function. Peroxiredoxins (PRDX) are abundant in human semen and important enzymes, which act as ROS scavengers modulators ROS‐dependent signaling. Our aim was to determine whether the levels PRDX1 PRDX6 their oxidation on thiol groups associated with motility DNA integrity. We evaluated seminal PRDX level men (13...
C-H alkenylation/annulation of benzimidates with alkynes has been realized by using a Cp*Co(III) catalyst under air. A series substituted isoquinolines were obtained moderate to good yields mild reaction conditions.
As fungal resistance to existing antifungal drugs continues rise, there is an urgent need for new with anti-resistance activity.
Abstract Background Aminoglycoside ototoxicity is one of the common health problems. Mitochondrial 12S rRNA mutations are important causes aminoglycoside ototoxicity. However, incidences associated with less known. Methods A total 440 Chinese pediatric hearing-impaired subjects were recruited from two otology clinics in Ningbo and Wenzhou cities Zhejiang Province, China. These underwent clinical, genetic evaluation molecular analysis mitochondrial rRNA. Resultant mtDNA variants evaluated by...
The deafness-associated m.12201T>C mutation affects the A5-U68 base-pairing within acceptor stem of mitochondrial tRNAHis. primary defect in this is an alteration tRNAHis aminoacylation. Here, we further investigate molecular mechanism 12201T>C and test whether overexpression human histidyl-tRNA synthetase gene (HARS2) cytoplasmic hybrid (cybrid) cells carrying reverses dysfunctions. Using dynamics simulations, demonstrate that perturbs structure function, supported by decreased melting...
Alternative splicing (AS) is important in the regulation of gene expression and aberrant AS emerging as a major factor pathogenesis human conditions, including cancer. Triple-negative breast cancer (TNBC) most challenging subtype with strong invasion, high rate metastasis, poor prognosis. Here we report systematic profiling TNBC.The percent spliced (PSI) values for events 151 TNBC patients were obtained from The Cancer Genome Atlas (TCGA) SpliceSeq database. Univariate Cox stepwise...
Abstract Oxidative phosphorylation (OXPHOS) and fatty acid oxidation (FAO) are key bioenergetics pathways. The machineries for both processes localized in mitochondria. Secondary OXPHOS defects have been documented patients with primary FAO deficiencies, vice versa. However, the underlying mechanisms remain unclear. Intrigued by observations that regulation of supercomplexes (SCs) assembly a mouse deficient cell line its derivatives is associated changes lipid metabolism, proteomics analysis...
Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that caused by Echinococcus granulosus (Eg). At present, serological test an important diagnostic method for Eg infection domestic animals. Here, fusion protein mefAg-1 harboring 8 dominant B-cell epitopes of such as antigen B, tetraspanin 1, 6, reticulon and Eg95 was produced E. coli evaluated CE indirect ELISA. showed ELISA high sensitivity (93.41%) specificity (99.31%), with coincidence rate 97.02%. Overall, it...
Abstract Fasciolosis is a zoonotic parasitic disease that seriously endangers the development of animal husbandry and human health. In order to develop rapid serological diagnostic method for fasciolosis in ruminants, CatL1D CatB4 genes Fasciola hepatica were amplified by reverse transcription polymerase chain reaction (PCR) cloned, respectively, then CatL-B fusion gene (MeCatL-B) was constructed splicing overlap extension PCR technique. The recombinant rCatL1D, rCatB4 rMeCatL-B proteins...
Chitinase AO-801 is a hydrolase secreted by <i>Arthrobotrys oligospora</i> during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase (reAO-801). belongs to typical glycoside 18 family with conserved sequence and tertiary structure (α/β)<sub>8</sub> triose-phosphate isomerase (TIM) barrel. The weight reAO-801 was 42 kDa. effectively degraded colloidal powdered chitin, egg lysate, stage I larval...
<title>Abstract</title> <bold>Background: </bold>Prolyl 4-hydroxylase beta polypeptide, encoded by the P4HB gene, belongs to protein disulfide isomerases family, implicated in multiple risk factor biological processes related occurrence of adverse pregnancy outcomes and embryonic development. Changes gene expression play an important role pathophysiologic processes. Little is known about regulation endogenous especially through selective splicing. In present study, we investigated...
Chitinase is an important virulence factor produced by nematode trapping fungi in the process of infection, and plays role cleavage nematodes their eggshells.In this study, cDNA sequence Arthrobotrys oligospora chitinase AO-379 was amplified RT-PCR inserted into vector pPIC9K to induce expression Pichia pastoris GS115.The recombinant (reAO-379) purified nickel ion affinity chromatography, enzymological properties nematode-degrading activity reAO-379 analyzed.SDS-PAGE Western blot analysis...
Mutations in mitochondrial tRNA genes have been shown to be associated with maternally inherited syndromic and non-syndromic deafness. Among those, mutations such as tRNALeu(UUR) 3243A>G deafness are often present heteroplasmy, the deafness-associated including tRNASer(UCN) 7445A>G homoplasmy or high levels of heteroplasmy. These primary factors underlying development hearing loss. However, other tRNAThr 15927G>A 7444G>A insufficient produce a phenotype, but always act synergy DNA mutations,...
Objective To study the effect of mitochondrial 12S rRNA mutations on aminoglycoside-induced and nonsyndromic hearing loss, to carry out clinical molecular characterization five Han Chinese pedigrees with maternally transmitted loss. Methods Five loss were collected, genomic DNA was extracted, complete genomes gap junction protein, beta 2 (GJB2) gene amplified sequenced. Results Clinical evaluation revealed a wide range severity, age-at-onset audiometric configuration impairment in...
Objective To investigate the clinical application of multiplex allele-specific PCR assays for simultaneous detection mitochondrial 12S rRNA A1555G and C1494T mutations associated with aminoglycoside-induced hearing impairment.Methods Three standard plasmids different genotypes (wild-type, mutant mutant) were constructed templates primers aiming directly at wild-type DNA nt1555 nt1494 designed developing a technique to detect mutations.Then method was applied screening 138 non-syndromic loss...
The mitochondrial 12 S rRNA is a hot spot for mutations associated with both aminoglycoside-induced and nonsyndromic hearing loss. Of those, the homoplasmic 1555A ＞ G 1494C T at highly conserved decoding region of have been loss.These two account significant number cases aminoglycoside ototoxicity. 15 5 A ＞G or mutation expected to form novel 1494C-G1555 1494U-A1555 base-pair A-site 12S rRNA. These transitions make human ribosome more bacteria-like, alter binding sites aminoglycosides,...