A5103202170- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Indoor Air Quality and Microbial Exposure
- Microbial Community Ecology and Physiology
- Air Quality Monitoring and Forecasting
- PI3K/AKT/mTOR signaling in cancer
- Angiogenesis and VEGF in Cancer
- Microbial metabolism and enzyme function
- Dental Research and COVID-19
- Pancreatic function and diabetes
- Trypanosoma species research and implications
- RNA regulation and disease
- Single-cell and spatial transcriptomics
- Electrospun Nanofibers in Biomedical Applications
- Bacterial Genetics and Biotechnology
- IL-33, ST2, and ILC Pathways
- Climate Change and Health Impacts
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolites in Food Biotechnology
- Corneal surgery and disorders
- Ophthalmology and Visual Impairment Studies
- Urinary Tract Infections Management
Tsinghua University
2014-2023
Center for Life Sciences
2017-2022
National Institute of Biological Sciences, Beijing
2020
National Tsing Hua University
1996
Particulate matter (PM) air pollution poses a formidable public health threat to the city of Beijing. Among various hazards PM pollutants, microorganisms in PM2.5 and PM10 are thought be responsible for allergies spread respiratory diseases. While physical chemical properties pollutants have been extensively studied, much less is known about inhalable microorganisms. Most existing data on airborne microbial communities using 16S or 18S rRNA gene sequencing categorize bacteria fungi into...
Worldwide, myopia is the leading cause of visual impairment. It results from inappropriate extension ocular axis and concomitant declines in scleral strength thickness caused by extracellular matrix (ECM) remodeling. However, identities initiators signaling pathways that induce ECM remodeling are unknown. Here, we used single-cell RNA-sequencing to identify activated sclera during development. We found hypoxia-signaling, eIF2-signaling, mTOR-signaling were murine myopic sclera. Consistent...
Usage of different types contact lenses is associated with increased risk sight-threatening complications. Changes in the ocular microbiome caused by lens wear are suggested to affect infection development those individuals. To address this question, study compares conjunctival microbial communities wearers noncontact wearers.Paired-end sequencing V3 region 16S rRNA gene was used characterize bacterial on surfaces and nonwearers.No differences diversity were detected between nonwearers....
Abstract Cells mitigate ER stress through the unfolded protein response (UPR). Here, we report formation of whorls as an effector mechanism response. We found that strong induces whorls, which contain ER-resident proteins such Sec61 complex and PKR-like kinase (PERK). whorl is dependent on PERK activity mediated by COPII machinery, facilitates membrane budding to form tubular-vesicular precursors. precursors then go Sec22b-mediated fusion whorls. further show contribute stress-induced...
The growth plate (GP) comprising sequentially differentiated cell layers is a critical structure for bone elongation and regeneration. Although several key regulators in GP development have been identified using genetic perturbation, systematic understanding still limited. Here, we used single-cell RNA-sequencing (RNA-seq) to determine the gene expression profiles of 217 single cells from GPs developed bioinformatics pipeline named Sinova de novo reconstruct physiological both temporal...
Abstract Fundamental processes such as ribosomal RNA synthesis and chromatin remodeling take place in the nucleolus, which is hyperactive fast-proliferating cells. The sophisticated regulatory mechanism underlying dynamic nucleolar structure functions yet to be fully explored. present study uncovers mutual functional dependency between a previously uncharacterized human long non-coding RNA, we renamed LETN , key protein, NPM1. Specifically, being upregulated multiple types of cancer, resides...
Abstract The quickly accumulating ribosome profiling data is an insightful resource for studying the critical details of translation regulation under various biological contexts. Rocaglamide A (RocA), antitumor heterotricyclic natural compound, has been shown to inhibit initiation a large group mRNA species by clamping eIF4A onto poly-purine motifs in 5′ UTRs. However, reanalysis previous datasets reveals unexpected shift occupancy pattern, upon RocA treatment types cells, during early...
Abstract Ketogulonicigenium vulgare has been widely used in vitamin C two steps fermentation and requires companion strain for optimal growth. However, the understanding of K. as well its is still preliminary. Here, complete genome Hbe602 was deciphered to provide insight into symbiosis mechanism versatile metabolism. contains LuxR family proteins, chemokine flagellar structure peptides transporters consortium. Besides, growth state metabolite variation were observed when five carbohydrates...
Mesenchymal stem cells (MSCs) show great promise in blood vessel restoration and vascularization enhancement many therapeutic situations. Typically, the co-implantation of MSCs with vascular endothelial (ECs) is effective for induction functional vivo, indicating its potential applications regenerative medicine. The effects MSCs-ECs-induced can be modeled vitro, providing simplified models understanding their underlying communication. In this article, a contact coculture model vitro an...
Hospital-acquired infection (HAI) or nosocomial is an issue that frequent hospital environment. We believe conventional regulated Petri dish method insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied screen airborne microbes in four rooms of Beijing Hospital. With air-in amount sampler being setup one person’s respiration quantity, identified huge numbers species the which had already qualified widely accepted petridish exposing standard, imposing...
Nanocrystalline structures were obtained by a rapid thermal annealing (RTA) process with heating rate of 600/spl deg/C/min for soaking time 2 minutes melt-spun Nd/sub 6/Fe/sub 88-x/MxB/sub 6/ amorphous alloys (M=Ti or V, x=0/spl sim/5), Exchange coupling interaction between the magnetically hard and soft phases was studied /spl delta/M plots. Addition Ti V to replace iron is effective in increasing remanence ratio, Mr/M/sub 2T/, energy product modified alloys, especially that although both...
Summary eIF3 is a multi-subunit complex with numerous functions in canonical translation initiation. was also found to interact 40S and 60S ribosomal proteins elongation factors, but direct involvement has never been demonstrated. We that deficiency reduced early speed between codons 25 75 on set of ∼2,700 mRNAs encoding associated mitochondrial membrane functions, resulting defective synthesis their encoded proteins. To promote elongation, interacts 80S ribosomes translating the first ∼60...