A5081361043- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Origins and Evolution of Life
- Photosynthetic Processes and Mechanisms
- Ultrasonics and Acoustic Wave Propagation
- Chemical Synthesis and Analysis
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Indoor Air Quality and Microbial Exposure
- Air Quality and Health Impacts
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Numerical methods in engineering
- Composite Material Mechanics
- Magnetic and Electromagnetic Effects
- Plant and Biological Electrophysiology Studies
- Ultrasound Imaging and Elastography
- Genetics, Aging, and Longevity in Model Organisms
- Antimicrobial Peptides and Activities
- Terahertz technology and applications
Aerospace Information Research Institute
2024
Chinese Academy of Sciences
2024
Westlake University
2022-2024
Zhejiang A & F University
2024
Shandong University
2012-2022
State Key Laboratory of Crystal Materials
2022
Peking University
2014-2022
Nanjing University of Aeronautics and Astronautics
2021-2022
Tsinghua University
2002-2021
Center for Life Sciences
2015-2021
Particulate matter (PM) air pollution poses a formidable public health threat to the city of Beijing. Among various hazards PM pollutants, microorganisms in PM2.5 and PM10 are thought be responsible for allergies spread respiratory diseases. While physical chemical properties pollutants have been extensively studied, much less is known about inhalable microorganisms. Most existing data on airborne microbial communities using 16S or 18S rRNA gene sequencing categorize bacteria fungi into...
The generation of synthetic forms cellular life requires solutions to the problem how biological processes such as cyclic growth and division could emerge from purely physical chemical systems. Small unilamellar fatty acid vesicles grow when fed with micelles can be forced divide by extrusion, but this artificial process results in significant loss protocell contents during each cycle. Here we describe a simple efficient pathway for model membrane division. large multilamellar micelles,...
Abstract The cloning of long DNA segments, especially those containing large gene clusters, is particular importance to synthetic and chemical biology efforts for engineering organisms. While has been a defining tool in molecular biology, the genome segments challenging. Here we describe technique that allows targeted near-arbitrary, bacterial genomic sequences up 100 kb be accomplished single step. target segment excised from chromosomes vitro by RNA-guided Cas9 nuclease at two designated...
The prominent pathological feature of fatty liver disease lesions is excessive fat accumulation in lipid droplets hepatocytes. Thus, developing fluorescent droplet-specific probes with high permeability and a imaging contrast provides robust tool for diagnosing diseases. Herein, we rationally developed novel donor-acceptor lipophilic probe ANI photostability wash-free visualization characteristics. showed typical twisted intramolecular charge transfer effect very faint fluorescence...
In recent research, metaheuristic strategies stand out as powerful tools for complex optimization, capturing widespread attention. This study proposes the Educational Competition Optimizer (ECO), an algorithm created diverse optimization tasks. ECO draws inspiration from competitive dynamics observed in real-world educational resource allocation scenarios, harnessing this principle to refine its search process. To further boost efficiency, divides iterative process into three distinct...
Prior to the evolution of complex biochemical machinery, growth and division simple primitive cells (protocells) must have been driven by environmental factors. We previously demonstrated two pathways for fatty acid vesicle in which initially spherical vesicles grow into long filamentous vesicles; is then mediated fluid shear forces. Here we describe a different pathway that independent external mechanical show illumination containing either fluorescent dye encapsulated aqueous phase, or...
Abstract Background While the physical and chemical properties of airborne particulate matter (PM) have been extensively studied, their associated microbiome remains largely unexplored. Here, we performed a longitudinal metagenomic survey 106 samples PM 2.5 10 in Beijing over period 6 months 2012 2013, including those from several historically severe smog events. Results We observed that composition functional potential were conserved between , although considerable temporal variations...
To synthesize a chirally inverted ribosome with the goal of building mirror-image biology systems requires preparation kilobase-long ribosomal RNAs that make up structural and catalytic core about two-thirds molecular mass ribosome. Here, we chemically synthesized 100-kilodalton T7 RNA polymerase, which enabled efficient faithful transcription full-length 5S, 16S, 23S from enzymatically assembled long genes. We further exploited versatile system for practical applications such as biostable...
Lipid droplets (LDs) containing cytosolic and nuclear LDs have recently received increasing attention because of their diverse biological roles in living systems. However, developing fluorescent probes for super-resolution visualization these subcellular still remains challenging due to insufficient fluorescence brightness poor membrane permeability. Herein, we rationally synthesized a series ultrabright solvatochromic based on benzoboranils (BBAs) LD-specific imaging using structured...
Life can persist under severe osmotic stress and low water activity in hypersaline environments. On Mars, evidence for the past presence of saline bodies is prevalent resulted widespread deposition sulfate chloride salts. Here we investigate Spotted Lake (British Columbia, Canada), a lake with extreme (>3 M) levels salts as an exemplar conditions thought to be associated ancient Mars. We provide first characterization microbial structure sediments through metagenomic sequencing, report...
Abstract Mirror-image aptamers made from chirally inverted nucleic acids are nuclease-resistant and exceptionally biostable, opening up opportunities for unique applications. However, the directed evolution selection of mirror-image directly large randomized l -DNA libraries has, to our knowledge, not been demonstrated previously. Here, we developed a ‘mirror-image selection’ scheme biostable with DNA polymerase. We performed iterative rounds enrichment polymerase chain reaction (PCR)...
Preparation of monodisperse vesicles is important both for research purposes and practical applications. While the extrusion through small pores (∼100 nm in diameter) results relatively uniform populations vesicles, to larger sizes very heterogeneous vesicles. Here we report a simple method preparing large multilamellar combination large-pore dialysis. For example, polydisperse 5-µm-diameter eliminates than 5 µm diameter. Dialysis extruded against 3-µm-pore-size polycarbonate membranes...
The construction of mirror-image biological systems may open the next frontier for biomedical technology development and discovery. Here we have designed chemically synthesized a mutant version thermostable Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) consisting d-amino acids. With total peptide length 358 amino acid residues, it is largest protein reported to date. We show that d-polymerase able amplify 120-bp l-DNA sequence coding Escherichia coli 5S ribosomal RNA gene rrfB by chain...
Abstract Polymerase chain reaction (PCR) has been a defining tool in modern biology. Towards realizing mirror-image PCR, we have designed and chemically synthesized mutant version of the 352-residue thermostable Sulfolobus solfataricus P2 DNA polymerase IV with l -amino acids tested its PCR activity biochemically. To best our knowledge, this enzyme is largest protein reported to date. We show that optimization conditions, fully synthetic capable amplifying template sequences up 1.5 kb. The...