A5101519051- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Signaling Pathways in Disease
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Genetics, Aging, and Longevity in Model Organisms
- Prenatal Screening and Diagnostics
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Microtubule and mitosis dynamics
- Fetal and Pediatric Neurological Disorders
- Heat shock proteins research
- NF-κB Signaling Pathways
- Cellular transport and secretion
- CAR-T cell therapy research
- Genomic variations and chromosomal abnormalities
- RNA regulation and disease
- Muscle Physiology and Disorders
- Plant Disease Resistance and Genetics
- BRCA gene mutations in cancer
Xiamen University
2012-2024
First Hospital of Shijiazhuang
2023
ZheJiang Academy of Agricultural Sciences
2023
Harvard University Press
2020
Harvard University
1996-2020
Anhui Normal University
2019
China Agricultural University
2005-2018
eIF3, whose subunits are frequently overexpressed in cancer, regulates mRNA translation from initiation to termination, but mRNA-selective functions of individual remain poorly defined. Using multiomic profiling upon acute depletion eIF3 subunits, we observed that while eIF3a, b, e, and f markedly differed their impact on holo-complex formation translation, they were each required for cancer cell proliferation tumor growth. Remarkably, eIF3k showed the opposite pattern with promoting global...
Ammonia is a major signal that regulates nitrogen fixation in most diazotrophs. Regulation of by ammonia the Gram-negative diazotrophs well-characterized. In these bacteria, this regulation occurs mainly at level nif (nitrogen fixation) gene transcription, which requires nif-specific activator, NifA. Although Gram-positive and diazotrophic Paenibacilli have been extensively used as bacterial fertilizer agriculture, how regulated response to availability bacteria remains unclear. An...
In-droplet cytokine capture assays combined with FACS to accurately identify and isolate activated immune cells.
The heterologous transcription factors NFAT and AP-1 coordinately regulate cytokine gene expression through cooperative binding to precisely juxtaposed DNA recognition elements. molecular origins of cooperativity in the are poorly understood. Herein we have used yeast one-hybrid screening alanine-scanning mutagenesis identify residues that affect interactions with on DNA. Mutation a single conserved Arg residue Ala cJun spacer region (R285A) led virtually complete abolition NFAT. DNA-binding...
The transcription factors nuclear factor of activated T cells (NFAT) and activator protein 1 (AP-1) coordinately regulate cytokine gene expression in T-cells by binding to closely juxtaposed sites promoters. structural basis for cooperative NFAT AP-1 these sites, indeed the composite regulatory elements general, is not well understood. Mutagenesis studies have identified a segment AP-1, which lies at junction its DNA-binding dimerization domains (basic region leucine zipper, respectively),...
The ARF tumor suppressor is a product of the INK4a/ARF locus, which frequently mutated in human cancer. expression up-regulated response to certain types DNA damage, oncogene activation, and interferon stimuli. Through interaction with p53 negative regulator MDM2, controls well-described p53/MDM2-dependent checkpoint. However, mechanism induction poorly understood. Using yeast two-hybrid screen, we identify novel ARF-interacting protein, N-Myc STATs interactor (NMI). Previously, NMI was...
The activated spindle assembly checkpoint (SAC) potently inhibits the anaphase-promoting complex/cyclosome (APC/C) to ensure accurate chromosome segregation at anaphase. Early studies have recognized that SAC should be silenced within minutes enable rapid APC/C activation and synchronous of chromosomes once all kinetochores are properly attached, but underlying silencers still being elucidated. Here, we report timely silencing in fission yeast requires dnt1 + , which causes severe...
Eukaryotic cells are constantly exposed to various environmental stimuli. It remains largely unexplored how cues bring about epigenetic fluctuations and affect heterochromatin stability. In the fission yeast Schizosaccharomyces pombe , heterochromatic silencing is quite stable at pericentromeres but unstable mating-type ( mat ) locus under chronic heat stress, although both loci within major constitutive regions. Here, we found that compromised gene elevated temperature linked...
The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of 69 increases sequence-specific DNA-binding activity by 103-fold, enabling methylated activate transcription methylation-resistance regulon. thiolate sulfur atom is coordinated tightly bound zinc ion N-terminal domain, and this metal−ligand interaction plays direct role promoting repair chemistry. thus founding member...
The pathogenic fungus Pestalotiopsis versicolor is a major etiological agent of fungal twig blight disease affecting bayberry trees. However, the lack complete genome sequence information for this crucial hinders molecular and genetic investigation its mechanism. To address knowledge gap, we have generated P. strain XJ27, employing combination Illumina, PacBio, Hi-C sequencing technologies. This comprehensive sequence, comprising 7 chromosomes with an N50 contig size 7,275,017 bp, GC content...
Summary eIF3 is a multi-subunit complex with numerous functions in canonical translation initiation. was also found to interact 40S and 60S ribosomal proteins elongation factors, but direct involvement has never been demonstrated. We that deficiency reduced early speed between codons 25 75 on set of ∼2,700 mRNAs encoding associated mitochondrial membrane functions, resulting defective synthesis their encoded proteins. To promote elongation, interacts 80S ribosomes translating the first ∼60...
In the fission yeast Schizosaccharomyces pombe, both RNAi machinery and RNAi-independent factors mediate transcriptional posttranscriptional silencing heterochromatin formation. Here, we show that of reporter genes at major native heterochromatic loci (centromeres, telomeres, mating-type locus rDNA regions) an artificially induced is alleviated in a hsp90 mutant, hsp90-G84C Also, H3K9me2 enrichment regions, especially subtelomeres, compromised, suggesting assembly defects. We further...
e17565 Background: BRCA1 and BRCA2 are tumour suppressor genes, involved in the homologous repair of double-stranded DNA breaks, located at 17q21.31 13q13.1, respectively. Germline BRCA1/2 mutations identified 13-15% ovarian cancers (OC), while an additional 5-7% harbor somatic among foreign populations. To determine landscape germline pathogenic alterations Chinese patients with cancer tested by next‐generation sequencing (NGS), aim defining best strategy to be implemented future routine...
ABSTRACT eIF3, whose subunits are frequently overexpressed in cancer, regulates mRNA translation from initiation to termination, but mRNA-selective functions of individual remain poorly defined. Using multi-omic profiling upon acute depletion eIF3 subunits, we observed that while eIF3a, b, e, and f markedly differed their impact on holo-complex formation translation, they were each required for cancer cell proliferation tumor growth. Remarkably, eIF3k showed the opposite pattern with...
ABSTRACT Eukaryotic cells are constantly exposed to various environmental stimuli. It remains largely unexplored how cues bring about epigenetic fluctuations and affect heterochromatin stability. In the fission yeast Schizosaccharomyces pombe , heterochromatic silencing is quite stable at pericentromeres but unstable mating-type ( mat ) locus under chronic heat stress, although both loci within major constitutive regions. Here, we found that compromised gene elevated temperature linked...
To investigate the perinatal clinical phenotype and genetic characteristics of two fetuses with ring chromosome 21 mosaicisms.Two who were diagnosed at Xiamen Maternal Child Health Care Hospital in November 2021 selected as study subjects. Clinical data collected. Conventional G-banded karyotyping chromosomal microarray analysis (CMA) carried out for their parents.Prenatal ultrasonography fetus 1 has revealed absence nasal bone, ventricular septal defect, persistent left superior vena cava,...