A5032106989- Metalloenzymes and iron-sulfur proteins
- Legume Nitrogen Fixing Symbiosis
- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Ammonia Synthesis and Nitrogen Reduction
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Microbial Fuel Cells and Bioremediation
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Algal biology and biofuel production
- Soil Carbon and Nitrogen Dynamics
- Phytase and its Applications
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Plant Growth Enhancement Techniques
- Chemical Reactions and Isotopes
- Animal Genetics and Reproduction
- Enzyme Production and Characterization
- Chromium effects and bioremediation
- Leaf Properties and Growth Measurement
China Agricultural University
2016-2025
Agrobiotechnology Institute
2025
University of Minnesota
2013-2015
University of Science and Technology of China
2007-2012
National Taiwan University Hospital
2011
Lakehead University
2011
Northeast Forestry University
2011
Institute of Intelligent Machines
2007
Abstract Paenibacillus polymyxa has widely been studied as a model of plant-growth promoting rhizobacteria (PGPR). Here, the genome sequences 9 P. strains, together with 26 other sequenced spp., were comparatively studied. Phylogenetic analysis concatenated 244 single-copy core genes suggests that strains and 5 isolated from diverse geographic regions ecological niches, formed closely related clade (here it is called Poly-clade). Analysis single nucleotide polymorphisms (SNPs) reveals local...
Most biological nitrogen fixation is catalyzed by molybdenum-dependent nitrogenase, an enzyme complex comprising two component proteins that contains three different metalloclusters. Diazotrophs contain a common core of nif genes encode the structural subunits and components required to synthesize However, complement enable diazotrophic growth varies significantly amongst fixing bacteria archaea. In this study, we identified minimal gene cluster consisting nine in genome Paenibacillus sp....
We provide here a comparative genome analysis of 31 strains within the genus Paenibacillus including 11 new genomic sequences N2-fixing strains. The heterogeneity genomes (15 and 16 non-N2-fixing strains) was reflected in large size shell genome, which makes up approximately 65.2% genes pan genome. Large numbers transposable elements might be related to heterogeneity. discovered that minimal compact nif cluster comprising nine nifB, nifH, nifD, nifK, nifE, nifN, nifX, hesA nifV encoding...
Paenibacillus is a large genus of Gram-positive, facultative anaerobic, endospore-forming bacteria. The currently comprises more than 150 named species, approximately 20 which have nitrogen-fixation ability. N2-fixing strains potential uses as bacterial fertilizer in agriculture. In this study, 179 were isolated by using nitrogen-free medium after heating at 85 °C for 10 min from 69 soil samples collected different plant rhizospheres areas. Of the strains, 25 had nifH gene encoding Fe...
Phosphorus (P) limits the production of maize, one major food crops in China. Phosphate-solubilizing bacteria (PSB) have capacity to solubilize phosphate complexes into plant absorbable and utilizable forms by process acidification, chelation, exchange reactions. In this study, six bacteria, including Paenibacillus sp. B1 strain, four Pseudomonas strains (B10, B14, SX1, SX2) Sphingobium SX14 were those isolated from maize rhizosphere identified based on their 16S rRNA sequences. All could...
In this study, 20-day-old soybean plants were watered with 100 mL of mM NaCl solution and sprayed silica nanoparticles (SiO2 NPs) or potassium silicate every 3 days over 15 days, a final dosage 12 mg SiO2 per plant. We assessed the alterations in plant's growth physiological traits, responses bacterial microbiome within leaf endosphere, rhizosphere, root endosphere. The result showed that type silicon did not significantly impact most plant parameters. However, communities endospheres had...
Nitrogen fixation has been established in protokaryotic model Escherichia coli by transferring a minimal nif gene cluster composed of 9 genes (nifB, nifH, nifD, nifK, nifE, nifN, nifX, hesA and nifV) from Paenibacillus sp. WLY78. However, the nitrogenase activity recombinant E. 78-7 is only 10 % that observed wild-type Paenibacillus. Thus, it necessary to increase through synthetic biology. In order heterologous host, total 28 selected WLY78 Klebsiella oxytoca were placed under control...
Diazotrophic bacteria can reduce N2 into plant-available ammonium (NH4+), promoting plant growth and reducing nitrogen (N) fertilizer requirements. However, there are few systematic studies on the effects of diazotrophic biological fixation (BNF) contribution rate host N uptake metabolism. In this study, interactions Paenibacillus beijingensis BJ-18 with wheat, maize, cucumber were investigated when it was inoculated to these seedlings grown in both low high soils, un-inoculated plants as...
Cucumber is an important vegetable crop in China. Fusarium wilt a soil-borne disease that can significantly reduce cucumber yields. Paenibacillus polymyxa WLY78 strongly inhibit oxysporum f. sp. Cucumerium, which causes disease. In this study, we screened the genome of and found eight potential antibiotic biosynthesis gene clusters. Mutation analysis showed among clusters, fusaricidin synthesis (fus) cluster involved inhibiting genus, Verticillium albo-atrum, Monilia persoon, Alternaria...
Phosphate (P) availability often limits biological nitrogen fixation (BNF) by diazotrophic bacteria. In soil, only 0.1% of the total P is available for plant uptake. solubilizing bacteria can convert insoluble to plant-available soluble (ionic and low molecular-weight organic P). However, limited information about effects synergistic application on nitrogenase activity rhizosphere nifH expression endosphere. this study, we investigated co-inoculation with a bacterium (Paenibacillus...
Five novel endospore-forming, nitrogen-fixing bacterial strains were isolated from the rhizosphere soils of plants species Sabina squamata , Weigela florida and Zanthoxylum simulans . A phylogenetic analysis based on 16S rRNA gene sequences revealed that five formed a distinct cluster within genus Paenibacillus These showed highest levels (96.2–98.2 %) sequence similarity with azotofixans However, DNA–DNA relatedness between these P. was 12.9–29.5 %. The DNA G+C contents found to be...
Paenibacillus polymyxa WLY78 is a nitrogen fixer and it can be potentially applied to biofertilizer in agriculture. In this study, P. labelled with gfp gene. The GFP-labelled used inoculate wheat, maize cucumber seedlings grown the gnotobiotic system soil, respectively. Observation by confocal laser scanning microscope reveals that GFP-labeled bacterial cells are mainly located on root surface epidermis of only few present within cortical cells. seedlings, were colonized epidermal cells,...
Biofertilizer is a good substitute for chemical fertilizer in sustainable agriculture, but its effects are often hindered by drought stress. Super absorbent polymer (SAP), showing capacity of water absorption and retention, can increase soil moisture. However, limited information available about the efficiency biofertilizer amended with SAP. This study was conducted to investigate synergistic application SAP biofertilizers (Paenibacillus beijingensis BJ-18 Bacillus sp. L-56) on plant growth,...
We tested the hypothesis that reducing carbon (C):Phosphorus (P) ratio in rhizosphere soil would reduce bacterial competition with plant for P from phytin, which then increase phytin use efficiency plant. A three-factor pot experiment was carried out to study effect of inoculation a phytin-mineralizing bacterium, Pseudomonas alcaligenes (PA), on maize uptake phytin. Two levels organic P, two inorganic and three different PA treatments were used. When plants grown low available transformed...
Ammonia is a major signal that regulates nitrogen fixation in most diazotrophs. Regulation of by ammonia the Gram-negative diazotrophs well-characterized. In these bacteria, this regulation occurs mainly at level nif (nitrogen fixation) gene transcription, which requires nif-specific activator, NifA. Although Gram-positive and diazotrophic Paenibacilli have been extensively used as bacterial fertilizer agriculture, how regulated response to availability bacteria remains unclear. An...
Body size is a key ecological trait of soil microorganisms related to their adaptation environmental changes. In this study, we reveal that the smaller show stronger community resistance than larger organisms in both maize and rice soil. Compared with organisms, have higher diversity broader niche breadth deploy survival strategies, because which they are less affected by selection thus survive complex various kinds environments. addition, strong correlation between ecosystem functions...
Most biological nitrogen fixation is catalyzed by molybdenum-dependent nitrogenase, an enzyme complex comprising two component proteins that contains three different metalloclusters.Diazotrophs contain a common core of nif genes encode the structural subunits and components required to synthesize metalloclusters.However, complement enable diazotrophic growth varies significantly amongst fixing bacteria archaea.In this study, we identified minimal gene cluster consisting nine in genome...
Xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. In present study, four neutral, thermostable xylanases (MtXyn11A, MtXyn11At, MtXyn11B, and MtXyn11C) from compost fungus Mycothermus thermophilus CGMCC3.18119 were overexpressed in Pichia pastoris GS115 used produce xylooligosaccharides beechwood xylan. The enzymes showed similar enzymatic properties (maximal activities at pH 6.0-6.5 65 °C) but varied catalytic efficiency cleaving actions....
Diazotrophic (nitrogen-fixing) Gram-positive and endospore-formed Paenibacillus spp. have potential uses as a bacterial fertilizer in agriculture. The transcriptional analysis of nitrogen fixation is lacking, although regulation mechanisms been well studied Gram-negative diazotrophs.Here we report global profiling sp. WLY78 cultured under N2-fixing condition (without O2 NH4(+)) non-N2-fixing (air 100 mM NH4(+)). nif (nitrogen fixation) gene operon composed 9 genes (nifBHDKENXhesAnifV) this...