A5103081920- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Viral gastroenteritis research and epidemiology
- Genomics and Chromatin Dynamics
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Insect and Arachnid Ecology and Behavior
- interferon and immune responses
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- RNA Interference and Gene Delivery
- Insect Utilization and Effects
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Livestock and Poultry Management
- Telomeres, Telomerase, and Senescence
- Plant Reproductive Biology
- Extracellular vesicles in disease
Institute of Biophysics
2017-2025
Chinese Academy of Sciences
2013-2025
Chengdu University
2025
Xichang University
2025
Institute of Zoology
2013-2018
Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we develop a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstruct tertiary reveal surprisingly “unentangled globule” conformation. We uncover many long-range duplexes higher-order junctions,...
RNA interference (RNAi) by introducing double-stranded (dsRNA) is a powerful approach to the analysis of gene function in insects; however, RNAi responses vary dramatically different insect species and tissues, underlying mechanisms remain poorly understood. The migratory locust, destructive pest hemimetabolic with panoistic ovaries, considered be highly susceptible via dsRNA injection, but its ovary appears completely insensitive. In present study, we showed that persisted only briefly...
Abstract Activation-induced cytidine deaminase (AID) mediates class switching by binding to a small fraction of single-stranded DNA (ssDNA) diversify the antibody repertoire. The precise mechanism for highly selective AID targeting in genome has remained elusive. Here, we report an RNA-binding protein, ROD1 (also known as PTBP3 ), that is both required and sufficient define AID-binding sites genome-wide activated B cells. interacts with via ultraconserved loop, which proves be critical...
ABSTRACT In addition to preventing insect metamorphosis, juvenile hormone (JH) is known stimulate aspects of reproduction. However, the molecular mechanisms JH action in reproduction remain largely unknown. By reanalyzing transcriptomic data from adults and other developmental stages migratory locust Locusta migratoria , we identified a gene coding for Kazal‐type protease inhibitor, previously named Greglin. Greglin specifically expressed adult females most abundant fat body ovaries....
1. The global diversity of domestic chicken breeds, each exhibiting unique and specialised traits, offers a compelling context to explore how selection influences genetic variation patterns. China, with its myriad local contributes significantly this diversity.
The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease levels occurs due to a translation. Since regulation at translational level is generally mediated through RNA-binding proteins, using RNA antisense purification coupled with mass spectrometry, identify novel mRNA-binding protein, namely, DDX1, sensitive palmitate treatment. Notably, knockdown or overexpression of DDX1 affects...
Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we developed a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstructed tertiary revealed surprisingly "unentangled globule" conformation. We uncovered many long-range duplexes higher-order...
Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we developed a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstructed tertiary revealed surprisingly "unentangled globule" conformation. We uncovered many long-range duplexes higher-order...
Abstract In the fission yeast Schizosaccharomyces pombe, Mei2, an RNA-binding protein essential for entry into meiosis, regulates meiosis initiation. Mei2 binds to a specific non-coding RNA species, meiRNA, and accumulates at sme2 gene locus, which encodes meiRNA. Previous research has shown that C-terminal recognition motif (RRM3) physically interacts with meiRNA 5′ region in vitro stimulates vivo. However, underlying mechanisms still remain elusive. We first employed crosslinking...
Virus-to-host RNA-RNA interactions directly regulate host mRNA stability and viral replication. However, globally profiling virus-to-host in situ remains challenging. Here, we present an RNA conformation sequencing (RIC-seq)-based protocol for mapping high-confidence infected cells. We detail steps formaldehyde crosslinking, pCp-biotin labeling, proximity ligation, chimeric enrichment, strand-specific library construction, data analysis. This allows unbiased identification of various viruses...
SARS-CoV-2 RNA interacts with host factors to suppress interferon responses and simultaneously induces cytokine release drive the development of severe coronavirus disease 2019 (COVID-19). However, how hijacks RNAs elicit such imbalanced immune remains elusive. Here, we analyzed in situ structures interactions infected cells patient lung samples using RIC-seq. We discovered that forms 973 potential duplexes 3'UTRs 142 mRNAs increase their stability by recruiting RNA-binding protein YBX3....