A5100425752- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA regulation and disease
- Cancer-related gene regulation
- Dermatologic Treatments and Research
- CRISPR and Genetic Engineering
- Wound Healing and Treatments
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Connective Tissue Growth Factor Research
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Heat shock proteins research
- Dermatological and Skeletal Disorders
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Genetic Neurodegenerative Diseases
- Fibroblast Growth Factor Research
- Hematopoietic Stem Cell Transplantation
- Lung Cancer Treatments and Mutations
- Lipid metabolism and biosynthesis
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
University of Chinese Academy of Sciences
2019-2025
First Affiliated Hospital of Xiamen University
2025
Shanghai East Hospital
2025
Zhejiang University
2013-2024
First Affiliated Hospital Zhejiang University
2021-2024
Fudan University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Zhongshan Hospital
2023
Sun Yat-sen University
2023
Cancer Hospital of Chinese Academy of Medical Sciences
2022
Abstract Hepatocellular carcinoma (HCC) is a major leading cause of cancer-related death worldwide. Alpha fetoprotein (AFP) reactivated in majority hepatocellular and associated with poor patient outcomes. Although increasing evidence has shown that AFP can regulate HCC cell growth, the precise functions hepatocarcinogenesis underlying mechanism remain incompletely understood. In this study, we demostrated depleting significantly suppressed diethylnitrosamine (DEN)-induced liver tumor...
Abstract LIN28 is an RNA binding protein with important roles in early embryo development, stem cell differentiation/reprogramming, tumorigenesis and metabolism. Previous studies have focused mainly on its role the cytosol where it interacts Let-7 microRNA precursors or mRNAs, few addressed LIN28’s within nucleus. Here, we show that displays dynamic temporal spatial expression during murine development. Maternal drops upon exit from 2-cell stage, zygotic induced at forming nucleolus 4-cell...
Hyperosmotic stress as physiologic dysfunction can reduce the cell volume and then redistribute both protein concentration ionic strength, but its effect on liquid-liquid phase separation (LLPS) is not well understood. Here, we map hyperosmotic-stress-induced nuclear LLPS of amyotrophic lateral sclerosis (ALS)-related proteins (fused in sarcoma [FUS], TAR DNA-binding 43 [TDP-43]). The dynamic reversibility FUS granules are continuable with increase hypertonic stimulation time, those TDP-43...
The exosome functions in the degradation of diverse RNA species, yet how it is negatively regulated remains largely unknown. Here, we show that NRDE2 forms a 1:1 complex with MTR4, nuclear cofactor critical for recruitment, via conserved MTR4-interacting domain (MID). Unexpectedly, mainly localizes speckles, where inhibits MTR4 recruitment and degradation, thereby ensures efficient mRNA export. Structural biochemical data revealed interacts MTR4's key residues, locks closed conformation,...
hnRNPA1 (heterogeneous nuclear ribonucleoprotein A1) plays a variety of roles in gene expression. However, little is known about the functional involvement vascular smooth muscle cell (VSMC) function and neointima hyperplasia. In this study, we have attempted to investigate contexts VSMC function, injury-induced vessel remodeling, human atherosclerotic lesions, as well discern molecular mechanisms involved. APPROACH AND RESULTS: expression levels were consistently modulated during phenotype...
Abstract LIN28A is important in somatic reprogramming and pluripotency regulation. Although previous studies addressed that can repress let-7 microRNA maturation the cytoplasm, few focused on its role within nucleus. Here, we show nucleolus-localized protein undergoes liquid-liquid phase separation (LLPS) mouse embryonic stem cells (mESCs) vitro. The RNA binding domains (RBD) intrinsically disordered regions (IDR) of contribute to other nucleolar proteins’ phase-separated condensate...
Abstract Background Enolase 1 (ENO1) is a conserved glycolytic enzyme that regulates glycolysis metabolism. However, its role beyond in the pathophysiology of multiple myeloma (MM) remains largely elusive. Herein, this study aimed to elucidate function ENO1 MM, particularly impact on mitophagy under bortezomib-induced apoptosis. Methods The bone marrow clinical MM patients and healthy normal donors was used compare expression level ENO1. Using online databases, we conducted an analysis...
Nuclear RNA decay has emerged as a mechanism for post-transcriptional gene regulation in cultured cells. However, whether this process occurs animals and holds biological relevance remains largely unexplored. Here, we demonstrate that MTR4, the central cofactor of nuclear exosome, is essential embryogenesis spermatogenesis. Embryonic development Mtr4 knockout mice arrests at 6.5 day. Germ cell-specific results male infertility with specific severe defect meiotic initiation. During...
Preserving hematopoietic stem cell (HSC) functionality is essential for maintaining healthy blood and the immune system throughout life. HSC function declines with age; however, underlying mechanisms are not fully understood. Using an inducible mosaic mouse model to overexpress transcription factor Bcl11a in compartment, we found that aging-related increase mitigated functional decline, promoted IL-1β production bone marrow (BM), accelerated attrition a non–cell-autonomous manner....
MicroRNAs (miRNAs) have been reported to play significant roles in the pathogenesis of various polyQ diseases. This study aims investigate regulation ATXN3 gene expression by miRNA. We found that miR‐25 reduced both wild‐type and polyQ‐expanded mutant ataxin‐3 protein levels interacting with 3′UTR mRNA. also increased cell viability, decreased early apoptosis, downregulated accumulation aggregates SCA3/MJD cells. These novel results shed light on potential role SCA3/MJD, provide a possible...
To investigate the functional involvements of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) in smooth muscle cell (SMC) differentiation from stem cells, embryonic cells were cultivated on collagen IV-coated plates to allow for SMC differentiation. We found that hnRNPA1 gene and protein expression was upregulated significantly during coexpressed with markers cell-derived SMCs as well 12.5 days mouse embryos. knockdown resulted downregulation transcription factors, while enforced...
Cell division cycle 5-like (CDC5L) protein is a cell regulator of the G2/M transition and has been reported to participate in catalytic step pre-messenger RNA (mRNA) splicing DNA damage repair. Recently, it was also found act as candidate oncogene osteosarcoma cervical tumors. However, role CDC5L expression tumor biology still unclear. Here, we analyzed clinical significance gliomas. The fresh glioma tissues paraffin-embedded slices evaluated by western blot immunohistochemistry,...
Rationale: RNA helicases, highly conserved enzymes, are currently believed to be not only involved in modulation, but also other biological processes. We recently reported that helicase DDX (DEAD-box protein)-5 is required for maintaining the homeostasis of vascular smooth muscle cells (SMCs). However, expression and function physiology disease unknown. Objective: To investigate role diseases. Methods Results: showed here DDX-5 was most abundant DEAD-box protein expressed human rodent...
Prp43p catalyzes essential steps in pre-mRNA splicing and rRNA biogenesis. In splicing, Spp382p stimulates the helicase to dissociate postcatalytic spliceosome and, some way, maintain integrity of assembly. Here we present a dosage interference assay identify Spp382p-interacting factors by screening for genes that when overexpressed specifically inhibit growth conditional lethal prp38-1 assembly mutant spp382-1 suppressor background. Identified, among others, are encoding established Prp8p,...
Significance To ensure efficient and accurate gene expression, pre-mRNA processing mRNA export need to be balanced. SF3b is a core component of U2 snRNP that participates in splicing 3′ pre-mRNAs. Here, we uncovered role promoting by directly binding with the mature recruiting key adaptor THO. Intriguingly, this not dependent on snRNP, but rather enhanced upon disruption as result SF3b–THO interaction recruitment mRNA. Together, our work uncovers an important suggests competition between...
Abstract Osteogenic sarcoma (OGS) is a primary bone cancer, characterized by aggressive neoplasm from mesenchymal oncogenesis. However, the clinical therapeutic regimen against OGS limited. Therefore, potential medication warrants to be further developed. Our previous study indicates that formononetin (FN) exerts effective pharmacological activity OGS. This aimed decipher molecular mechanism behind this benefit. Patients with were recruited for data assay and immunoassay. Human cell line...
The birth weight of chickens does not significantly affect the at slaughter, while different growth rate after was one important reasons for difference in slaughter weight. Also, increase chickens' postnatal skeletal muscle is main cause gain, but which genes are involved this biological process still unclear. In study, by integrating four transcriptome datasets containing chicken muscles developmental times or tissues public databases, a total nine candidate that may be related to...
Cancer cells that survive fractionated irradiation can be radioresistant and cause tumor recurrence. However, the molecular mechanisms underlying development of radioresistance in cancer remain elusive. The aim this study was to investigate role WISP-1 esophageal carcinoma during irradiation. Radioresistant were generated from normal via irradiation, expression levels related proteins determined by Western blot. Radiosensitivity established clonogenic cell survival assays, cycle distribution...