Abstract Background Recent studies indicate important roles for long noncoding RNAs (lncRNAs) in the regulation of gene expression by acting as competing endogenous (ceRNAs). However, specific role lncRNAs skeletal muscle atrophy is still unclear. Our study aimed to identify function that control myogenesis and atrophy. Methods RNA sequencing was performed transcriptome (lncRNA messenger RNA) between hypertrophic broilers leaner broilers. To ‘sponge’ lncRNA, we constructed a lncRNA‐microRNA...

This study investigates the immunological factors underlying differential susceptibility of two chicken strains, E- and M-lines, to avian leukosis virus subgroup J (ALV-J). During eradication at a breeder farm in Guangdong, we observed strain-specific differences ALV-J. Moreover, E-line chickens exhibited slower antibody response ALV-J compared M-line chickens. As T cell receptor (TCR) B (BCR) are critical for antigen recognition, their activation triggers specific immune responses,...

Fat deposits in chickens contribute significantly to meat quality attributes such as juiciness, flavor, taste and other organoleptic properties. The quantity of fat deposited increases faster earlier the fast-growing than slow-growing chickens. In this study, Affymetrix Genechip® Chicken Genome Arrays 32773 transcripts were used compare gene expression profiles liver hypothalamus tissues chicken at 8 wk age. Real-time RT-PCR was validate differential genes selected from microarray analysis....

Long noncoding RNAs (lncRNAs) play important roles in epigenetic regulation of skeletal muscle development. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-Six1 is an lncRNA differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. this study, have further demonstrated located 432 bp upstream the gene encoding protein Six homeobox 1 (Six1). A dual-luciferase reporter assay identified overlaps Six1 proximal promoter. lncRNA-Six1, a...

Chicken growth traits are important economic in broilers. A large number of studies available on finding genetic factors affecting chicken growth. However, most these identified chromosome regions containing putative quantitative trait loci and causal mutations is still a challenge. In this genome-wide association study (GWAS), we narrow 1.5 Mb region (173.5–175 Mb) (Gallus gallus) (GGA) 1 to be strongly associated with using 47,678 SNPs 489 F2 chickens. The included aggregate body weight...

Summary The proliferation, apoptosis, and differentiation of myoblasts are essential processes in skeletal muscle development. During this developmental process, microRNAs (miRNAs) play crucial roles. In our previous RNA-seq study (accession number GSE62971), we found that miR-16-5p was differentially expressed between fast slow growth chicken. study, report could inhibit myoblast promote repress by directly binding to the 3′ UTR SESN1 , which is also expressed. Overexpression significantly...

Circular RNAs (circRNAs), expressed abundantly and universally in various eukaryotes, are involved growth development of animals. Our previous study on circRNA sequencing revealed that circSVIL, an exonic circular, differentially among skeletal muscle at 11 embryo age (E11), 16 (E16), 1 day post-hatch (P1). In this study, we aim to investigate the effect circSVIL muscle. We detected expression level embryonic leg during E10 P1. As a result, found had high late Through dual-luciferase assay,...

The growth and development of skeletal muscle is regulated by proteins as well non-coding RNAs. Circular RNAs (circRNAs) are universally expressed in various tissues cell types, regulate gene expression eukaryotes. To identify the circRNAs during chicken embryonic development, leg muscles female Xinghua (XH) at three developmental time points 11 embryo age (E11), 16 (E16) 1 day post hatch (P1) were performed RNA sequencing. We identified 13,377 with 3,036 abundantly most derived from coding...

The fusion of myoblasts is an important step during skeletal muscle differentiation. A recent study in mice found that a transmembrane protein called Myomaker, which specifically expressed muscle, critical for myoblast fusion. However, the cellular mechanism its roles and regulatory expression remain unclear. Chicken not only plays role meat production but also ideal model organism development research. Here, we report Myomaker essential chicken Forced primary promotes fusion, whereas...

The differences in physiological and immunological parameters pathological damage to organ tissues exposed chronic heat stress provide the basis for evaluating resistance of different chicken breeds (white recessive rock [WRR] Lingshan [LS]). Ninety broilers each breed were divided equally into a group no group. effects on analyzed using flow cytometry, ELISA, RT-qPCR, etc. Under conditions: (1) H H/L values significantly increased (P < 0.01) 2 breeds, higher WRR than LS at late stage 0.05)....

Transmembrane proteins are vital for intercellular signalling and play important roles in the control of cell fate. However, their physiological functions mechanisms action myogenesis muscle disorders remain largely unexplored. It has been found that transmembrane protein 182 (TMEM182) is dramatically up-regulated during myogenesis, but its detailed unclear. This study aimed to analyse function TMEM182 regeneration.RNA sequencing, quantitative real-time polymerase chain reaction,...

Abstract Background Skeletal muscle development is pivotal for animal growth and health. Recently, long noncoding RNAs (lncRNAs) were found to interact with chromatin through diverse roles. However, little known about how lncRNAs act as chromatin-associated regulate skeletal development. Here, we aim investigate the regulation of RNA (MYH1G-AS) during Methods We provided comprehensive insight into profile accessibility different myofibers, combining sequencing (RNA-seq) an assay...

Introduction Growth traits are important in poultry production, however, little is known for its regulatory mechanism at epigenetic level. Therefore, this study, we aim to compare DNA methylation profiles between fast- and slow-growing broilers order identify candidate genes chicken growth. Methylated immunoprecipitation-sequencing (MeDIP-seq) was used investigate the genome-wide pattern high low tails of Recessive White Rock (WRRh; WRRl) that Xinhua Chickens (XHh; XHl) 7 weeks age. The...

HMIHC strategy is favorable to develop the novel nanodrug ferumoxytol with flexible magnetism and will boost clinical translation of inorganic nanodrugs.

Long non-coding RNAs (lncRNAs) play important roles in transcriptional and post-transcriptional regulation. However, little is currently known about the mechanisms by which they regulate skeletal muscle development chicken. In this study, we used RNA sequencing to profile leg transcriptome (lncRNA mRNA) at three stages of chicken: embryonic day 11 (E11), 16 (E16), 1 after hatching (D1). total, 129, 132, 45 differentially expressed lncRNAs, 1798, 3072, 1211 mRNAs were identified comparisons...

Abstract miR-17 family microRNAs (miRNAs) are crucial for embryo development, however, their role in muscle development is still unclear. miR-20a-5p and miR-20b-5p belong to the transcribed from miR-17~92 miR-106a~363 clusters respectively. In this study, we found that promoted myoblast differentiation repressed proliferation by directly binding 3′ UTR of E2F transcription factor 1 ( E2F1 ) mRNA. an important transcriptional organism’s normal development. Overexpression myoblasts inhibited...

The feed conversion ratio (FCR) and residual intake (RFI) are common indexes in measuring efficiency for livestock. RFI is a adjusted requirements maintenance production so these two traits related. Similarly, FCR related to weight gain because it their ratio. Cholecystokinin type A receptor (CCKAR) plays an important role animal digestive process. We examined the interplay of three parameters local Chinese chicken population. (FI) body weights (BW) 1,841 individuals were monitored on daily...

Abstract Avian leukosis virus subgroup J (ALV-J) can cause several different leukemia-like proliferative diseases in the hemopoietic system of chickens. Here, we investigated transcriptome profiles and miRNA expression ALV-J-infected uninfected chicken spleens to identify genes miRNAs related ALV-J invasion. In total, 252 167 were differentially expressed compared control spleens. miR-23b was up-regulated with analysis revealed that interferon regulatory factor 1 ( IRF1 ) down-regulated A...

Residual feed intake (RFI) is a powerful indicator for energy utilization efficiency and responds to selection. Low RFI selection enables reduction in without affecting growth performance. However, the effective variants or major genes dedicated phenotypic differences quality chickens are unclear. Therefore, genome-wide association study (GWAS) RNA sequencing were performed on identify genetic potential candidate associated with improvement. A lower average daily was found low-RFI birds...