Abstract Background Recent studies indicate important roles for long noncoding RNAs (lncRNAs) in the regulation of gene expression by acting as competing endogenous (ceRNAs). However, specific role lncRNAs skeletal muscle atrophy is still unclear. Our study aimed to identify function that control myogenesis and atrophy. Methods RNA sequencing was performed transcriptome (lncRNA messenger RNA) between hypertrophic broilers leaner broilers. To ‘sponge’ lncRNA, we constructed a lncRNA‐microRNA...

Long noncoding RNAs (lncRNAs) play important roles in epigenetic regulation of skeletal muscle development. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-Six1 is an lncRNA differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. this study, have further demonstrated located 432 bp upstream the gene encoding protein Six homeobox 1 (Six1). A dual-luciferase reporter assay identified overlaps Six1 proximal promoter. lncRNA-Six1, a...

Summary The proliferation, apoptosis, and differentiation of myoblasts are essential processes in skeletal muscle development. During this developmental process, microRNAs (miRNAs) play crucial roles. In our previous RNA-seq study (accession number GSE62971), we found that miR-16-5p was differentially expressed between fast slow growth chicken. study, report could inhibit myoblast promote repress by directly binding to the 3′ UTR SESN1 , which is also expressed. Overexpression significantly...

The growth and development of skeletal muscle is regulated by proteins as well non-coding RNAs. Circular RNAs (circRNAs) are universally expressed in various tissues cell types, regulate gene expression eukaryotes. To identify the circRNAs during chicken embryonic development, leg muscles female Xinghua (XH) at three developmental time points 11 embryo age (E11), 16 (E16) 1 day post hatch (P1) were performed RNA sequencing. We identified 13,377 with 3,036 abundantly most derived from coding...

Long non-coding RNAs (lncRNAs) play important roles in transcriptional and post-transcriptional regulation. However, little is currently known about the mechanisms by which they regulate skeletal muscle development chicken. In this study, we used RNA sequencing to profile leg transcriptome (lncRNA mRNA) at three stages of chicken: embryonic day 11 (E11), 16 (E16), 1 after hatching (D1). total, 129, 132, 45 differentially expressed lncRNAs, 1798, 3072, 1211 mRNAs were identified comparisons...

Abstract Skeletal muscle differentiation can be regulated by various transcription factors and non-coding RNAs. In our previous work, miR-223 is differentially expressed in the skeletal of chicken with different growth rates, but its role, expression action mechanism development still remains unknown. Here, we found that MYOD factor upregulate binding to an E-box region gga-miR-223 gene promoter during avian myoblast differentiation. IGF2 ZEB1 are two target genes miR-223. The inhibition on...

Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate Six1 cis to participate myogenesis. Here, studied a microRNA (miRNA) specifically expressed chickens (miR-1611). Interestingly, miR-1611 was contain potential binding sites both and Six1, it interact with expression. Overexpression of represses the proliferation...

Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that has a similar replication cycle to multiple viruses and therefore can be used as model system for viral entry into host cells. However, there are few reports on the genes or microRNAs (miRNAs) responsible of ALV-J. Our previous miRNA RNA sequencing data showed expression miR-34b-5p was significantly upregulated in ALV-J-infected chicken spleens compared non-infected spleens, but melanoma differentiation-associated gene 5...

As the world population grows, muscle atrophy leading to wasting could become a bigger risk. Long noncoding RNAs (lncRNAs) are known play important roles in growth and atrophy. Meanwhile, it has recently come light that many putative small open reading frames (sORFs) hidden lncRNAs; however, their translational capabilities functions remain unclear. In this study, we uncovered 104 myogenic-associated lncRNAs translated, at least peptide, by integrated transcriptome proteomic analyses....

Abstract In the poultry industry, aggressive behaviour is a large animal welfare issue all over world. To date, little known about underlying genetics of behaviour. Here, we performed genome-wide association study (GWAS) to explore genetic mechanism associated with in chickens. The GWAS results showed that total 33 SNPs were traits ( P < 4.6E-6). rs312463697 on chromosome 4 was significantly aggression = 2.10905E-07) and it intron region sortilin-related VPS10 domain containing receptor 2...

Myogenesis mainly involves several steps including myoblast proliferation, differentiation, apoptosis and fusion. Except for muscle specific regulators, few miRNAs were proved to coordinate this complex process. Here, we reported that miR-16 inhibited proliferation promoted by directly targeting Bcl2 FOXO1. The expression level of was significantly decreased in the hypertrophic pectoral compared normal chicken. In vitro, elevating apoptosis, resulting about 11.2% cells arrested G1 phase...

As key post-transcriptional regulators, microRNAs (miRNAs) play an indispensable role in skeletal muscle development. Our previous study suggested that miR-34b-5p and IGFBP2 could have a potential growth. goal this is to explore the function regulatory mechanism of myogenesis. In study, dual-luciferase reporter assay Western blot analysis showed direct target miR-34b-5p. Flow cytometric EdU repress cell cycle progression myoblasts, promote formation myotubes by promoting expression MyHC. On...

Abstract Skeletal muscle is the largest metabolic organ in body, and its flexibility essential for maintaining systemic energy homeostasis. Metabolic inflexibility muscles a dominant cause of various disorders, impeding development. In our previous study, we found lncRNA ZFP36L2-AS (for “ ZFP36L2 -antisense transcript”) specifically enriched skeletal muscle. Here, report that upregulated during myogenic differentiation, highly expressed breast leg vitro, inhibits myoblast proliferation but...

MicroRNAs (miRNAs) are non-coding RNAs that regulate mRNA expression by degradation or translational inhibition. We investigated the underlying molecular mechanisms of skeletal muscle development based on differentially expressed genes and miRNAs. compared miRNA from chicken at embryonic day E11, E16 one post-hatch (P1). The interaction networks were constructed, according to target prediction results integration analysis up-regulated with down regulated miRNAs down-regulated |log2fold...

miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter miR-206 chicken found that preferable was located 1200 bp upstream pri-miR-206. In this region, many key transcription factors, including MyoD, c-Myb, CEBPα/β, AP-4, RAP1, Brn2, GATA-1/2/3, E47, Sn, stimulatory factor (USF) CdxA, were predicted to bind interact with promoter. Overexpression MyoD sharply...

Abstract Obesity is a global epidemic health disorder and associated with several diseases. Body weight–reducing effects of melatonin have been reported; however, no investigation toward examining whether the beneficial are preadipocyte heterogeneity has reported. In this study, we profiled 25 071 transcriptomes normal melatonin‐treated preadipocytes using scRNA‐seq. By tSNE analysis, present cellular‐state landscape for that covers multiple‐cell subpopulations, defined as cluster 0 to 13....

As societal progress elevates living standards, the focus on meat consumption has shifted from quantity to quality. In broiler production, optimizing quality become paramount, prompting efforts refine various attributes. Recent advancements in sequencing technologies have revealed genome’s complexity, surpassing previous conceptions. Through experimentation, numerous genetic elements been linked crucial traits chickens. This review synthesizes current understanding of determinants associated...

Non-coding RNAs especially miRNAs have been found to play important roles during skeletal muscle development. Our previous RNA-Seq performed on breast tissue from 7 weeks old Recessive White Rock and Xinhua Chicken leg female Xinghua at three development time points (11 embryo age, 16 1 day post hatch) (accession number GSE62971 GSE89355, respectively) showed that miR-205a CDH11 were differentially expressed genes. In this study, we overexpression of significantly facilitated Quail clone...

The aim of this study was to investigate the feasibility stimulating ovarian follicle development in order improve fertility water buffalo cows by immunization against inhibin. experiment carried out early summer (May) and included 24 multi-parity crossbred Murrah-Swamp buffaloes that were divided into immunized (n=11) control (n=13) groups. Each cow administered with a 2-mL immunogen mineral oil adjuvant containing 2 mg recombinant inhibin α-subunit fusion protein. controls treated only....