A5101510516- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- MicroRNA in disease regulation
- Genetic and phenotypic traits in livestock
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Neuroendocrine regulation and behavior
- Genetic Mapping and Diversity in Plants and Animals
- Animal Nutrition and Physiology
- interferon and immune responses
- Muscle Physiology and Disorders
- Genomics and Chromatin Dynamics
- Stress Responses and Cortisol
- Advanced Nanomaterials in Catalysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Sensors and Ion Detection
- Body Composition Measurement Techniques
- Metal-Organic Frameworks: Synthesis and Applications
- Electric and Hybrid Vehicle Technologies
- CRISPR and Genetic Engineering
- Electric Vehicles and Infrastructure
- Regulation of Appetite and Obesity
- Circadian rhythm and melatonin
South China Agricultural University
2016-2025
Ocean University of China
2024-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Wuhan Institute of Technology
2022-2024
Dalian University of Technology
2022
Rockefeller University
2020-2021
Northeast Agricultural University
2020-2021
Education Department of Heilongjiang Province
2020-2021
Shandong Jiaotong University
2021
Jiangsu Normal University
2020
Abstract Background Recent studies indicate important roles for long noncoding RNAs (lncRNAs) in the regulation of gene expression by acting as competing endogenous (ceRNAs). However, specific role lncRNAs skeletal muscle atrophy is still unclear. Our study aimed to identify function that control myogenesis and atrophy. Methods RNA sequencing was performed transcriptome (lncRNA messenger RNA) between hypertrophic broilers leaner broilers. To ‘sponge’ lncRNA, we constructed a lncRNA‐microRNA...
Long noncoding RNAs (lncRNAs) play important roles in epigenetic regulation of skeletal muscle development. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-Six1 is an lncRNA differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. this study, have further demonstrated located 432 bp upstream the gene encoding protein Six homeobox 1 (Six1). A dual-luciferase reporter assay identified overlaps Six1 proximal promoter. lncRNA-Six1, a...
Summary The proliferation, apoptosis, and differentiation of myoblasts are essential processes in skeletal muscle development. During this developmental process, microRNAs (miRNAs) play crucial roles. In our previous RNA-seq study (accession number GSE62971), we found that miR-16-5p was differentially expressed between fast slow growth chicken. study, report could inhibit myoblast promote repress by directly binding to the 3′ UTR SESN1 , which is also expressed. Overexpression significantly...
Circular RNAs (circRNAs), expressed abundantly and universally in various eukaryotes, are involved growth development of animals. Our previous study on circRNA sequencing revealed that circSVIL, an exonic circular, differentially among skeletal muscle at 11 embryo age (E11), 16 (E16), 1 day post-hatch (P1). In this study, we aim to investigate the effect circSVIL muscle. We detected expression level embryonic leg during E10 P1. As a result, found had high late Through dual-luciferase assay,...
The growth and development of skeletal muscle is regulated by proteins as well non-coding RNAs. Circular RNAs (circRNAs) are universally expressed in various tissues cell types, regulate gene expression eukaryotes. To identify the circRNAs during chicken embryonic development, leg muscles female Xinghua (XH) at three developmental time points 11 embryo age (E11), 16 (E16) 1 day post hatch (P1) were performed RNA sequencing. We identified 13,377 with 3,036 abundantly most derived from coding...
Phenylglyoxylic acid (PGA), the metabolite of styrene after its invasion into human body, must be monitored conveniently and accurately. Herein, two zinc-based metal–organic frameworks {[Zn(H2L)bimb]}n (Zn-1) {[Zn2(H2L)2bipd]}n (Zn-2) [H4L = 1,2,4,5-tetrakis(4-carboxyphenyl)-3,6-dimethyl-benzene, bimb 1,1-(1,4-butanediyl)bis(imidazole), bipd 3,5-bis(1-imidazole)pyridine] had been obtained by solvothermal method could used for selective detection PGA with an extremely low limit....
Introduction Growth traits are important in poultry production, however, little is known for its regulatory mechanism at epigenetic level. Therefore, this study, we aim to compare DNA methylation profiles between fast- and slow-growing broilers order identify candidate genes chicken growth. Methylated immunoprecipitation-sequencing (MeDIP-seq) was used investigate the genome-wide pattern high low tails of Recessive White Rock (WRRh; WRRl) that Xinhua Chickens (XHh; XHl) 7 weeks age. The...
Long non-coding RNAs (lncRNAs) play important roles in transcriptional and post-transcriptional regulation. However, little is currently known about the mechanisms by which they regulate skeletal muscle development chicken. In this study, we used RNA sequencing to profile leg transcriptome (lncRNA mRNA) at three stages of chicken: embryonic day 11 (E11), 16 (E16), 1 after hatching (D1). total, 129, 132, 45 differentially expressed lncRNAs, 1798, 3072, 1211 mRNAs were identified comparisons...
Abstract Avian leukosis virus subgroup J (ALV-J) can cause several different leukemia-like proliferative diseases in the hemopoietic system of chickens. Here, we investigated transcriptome profiles and miRNA expression ALV-J-infected uninfected chicken spleens to identify genes miRNAs related ALV-J invasion. In total, 252 167 were differentially expressed compared control spleens. miR-23b was up-regulated with analysis revealed that interferon regulatory factor 1 ( IRF1 ) down-regulated A...
Embryonic growth and development of skeletal muscle is a major determinant mass, has significant effect on meat production in chicken. To assess the protein expression profiles during embryonic development, we performed proteomics analysis using isobaric tags for relative absolute quantification (iTRAQ) leg tissues female Xinghua chicken at age (E) 11, E16, 1-day post hatch (D1). We identified 3,240 proteins 491 them were differentially expressed (fold change ≥ 1.5 or ≤ 0.666 p-values <...
Abstract Gamecock chickens are one of the earliest recorded birds in China, and have accumulated some unique morphological behavioral signatures such as large body size, muscularity aggressive behavior, whereby being excellent breeding materials a good model for studying bird muscular development behavior. In this study, we sequenced 126 chicken genomes from 19 populations, including four commercial breeds that commonly farmed 13 nationwide Chinese typical indigenous (including two gamecock...
Abstract Chicken is a valuable model for understanding fundamental biology, vertebrate evolution and diseases, as well major source of nutrient-dense lean-protein-enriched food globally. Although it the first non-mammalian amniote genome to be sequenced, chicken still lacks systematic characterization functional impacts genetic variants. Here, through integrating 7,015 RNA-Seq 2,869 whole-genome sequence data, Genotype- Tissue Expression (ChickenGTEx) project presents pilot reference...
In chicken breeding, body fat distribution is an important genetic indicator. To address the issues of low accuracy, high workload, and limited experimental environment in traditional measurement methods, this paper proposes online method for live based on principle bio-electrical impedance. The system uses STM32F103C8T6 processor, AD5933 impedance module, four-electrode sampling core module to design system. During detection process, electrode was placed left chest-left tibia broilers, a...
Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate Six1 cis to participate myogenesis. Here, studied a microRNA (miRNA) specifically expressed chickens (miR-1611). Interestingly, miR-1611 was contain potential binding sites both and Six1, it interact with expression. Overexpression of represses the proliferation...
Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that has a similar replication cycle to multiple viruses and therefore can be used as model system for viral entry into host cells. However, there are few reports on the genes or microRNAs (miRNAs) responsible of ALV-J. Our previous miRNA RNA sequencing data showed expression miR-34b-5p was significantly upregulated in ALV-J-infected chicken spleens compared non-infected spleens, but melanoma differentiation-associated gene 5...
Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional methylation changes related to host response APEC among groups non-challenged chickens (NC), challenged with mild (MD) severe pathology (SV). was enriched the gene bodies repeats. Promoter CGIs hypomethylated. Integration analysis revealed 22, 87, 9 genes exhibiting inversely changed expression NC vs. MD, SV, MD...
As the world population grows, muscle atrophy leading to wasting could become a bigger risk. Long noncoding RNAs (lncRNAs) are known play important roles in growth and atrophy. Meanwhile, it has recently come light that many putative small open reading frames (sORFs) hidden lncRNAs; however, their translational capabilities functions remain unclear. In this study, we uncovered 104 myogenic-associated lncRNAs translated, at least peptide, by integrated transcriptome proteomic analyses....
Abstract Long non-coding RNAs (lncRNAs) are well-known to participate in a variety of important regulatory processes myogenesis. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-FKBP1C was differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. Here, have further demonstrated interacted directly with MYH1B by biotinylated RNA pull-down assay immunoprecipitation (RIP). Protein stability degradation experiments identified enhanced...
Abstract In actual industrial processes, numerous cantilever structures with free ends carry heavy loads weighing several tons. For such structures, fixed supports are often added at the load end, forming a double-supported structure to reduce large forces and moments imposed on by load. However, when end of undergoes deflection due changes in operating conditions, significant stress is generated structure, severely affecting its mechanical lifespan safety. A follow-up support scheme needs...
The G0/G1 switch gene 2 (G0S2) has been shown to be involved in cell proliferation, apoptosis, and differentiation mammals. However, its function poultry is not fully understood, especially preadipocytes of chickens. This study aimed establish a G0S2 knockout preadipocyte line chickens through CRISPR/Cas9 technology thoroughly investigate the impact on chicken differentiation. To explore involvement growth development, transcriptome sequencing was performed. results demonstrated that...
Two zinc (II)-based coordination polymers namely, {[Zn2L2(bmib)]·DMA}n (1) and {[ZnL(bipd)]}n (2). (H2L= 5-{(anthracen-9-ylmethyl)amino}isophthalic acid, bmib= 1,4-bis(2-methyl-imidazol-1-yl)butane, bipd= 3,5-bi-1H-imidazol-1-ylpyridine, DMA= N,N-dimethylacetamide), were synthesized by hydrothermal method. Single-crystal X-ray diffraction analysis showed...