A5019117303- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- Animal Nutrition and Physiology
- Connective tissue disorders research
- Nutrition, Genetics, and Disease
- interferon and immune responses
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Estrogen and related hormone effects
- Genomics and Chromatin Dynamics
- Kruppel-like factors research
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Meat and Animal Product Quality
- TGF-β signaling in diseases
- Hypothalamic control of reproductive hormones
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
China Agricultural University
2023-2024
Shandong Agricultural University
2020-2023
Ministry of Agriculture and Rural Affairs
2023
A comprehensive characterization of regulatory elements in the chicken genome across tissues will have substantial impacts on both fundamental and applied research. Here, we systematically identified characterized by integrating 377 genome-wide sequencing datasets from 23 adult tissues. In total, annotated 1.57 million elements, representing 15 distinct chromatin states, predicted about 1.2 enhancer-gene pairs 7662 super-enhancers. This functional annotation should wide utility identifying...
The industry of egg-type chicken has shown a trend extending the rearing period, with goal breeding breeds capable producing 500 qualified eggs by 700 d age. However, rapid decline in eggshell quality during late laying period is one major challenges. In this study, total 3,261 Rhode Island Red chickens were used to measure traits including strength (ESS), thickness (EST), color (ESC) and gloss (ESG) at seven age points ranging from 36 90 wk Phenotypic variations increased aging process,...
Abstract Chicken is a valuable model for understanding fundamental biology, vertebrate evolution and diseases, as well major source of nutrient-dense lean-protein-enriched food globally. Although it the first non-mammalian amniote genome to be sequenced, chicken still lacks systematic characterization functional impacts genetic variants. Here, through integrating 7,015 RNA-Seq 2,869 whole-genome sequence data, Genotype- Tissue Expression (ChickenGTEx) project presents pilot reference...
In chickens, follicle selection is an important process affecting laying traits, which characterized by the differentiation of granulosa cells and synthesis progesterone from hierarchical follicles. By using Oxford Nanopore Technologies (ONT) approach, we compared transcriptomes between pre-hierarchical (Pre-GCs) follicles (Post-GCs) to identify genes underlying chicken selection. A total 2,436 differentially expressed (DEGs), 3,852 transcripts (DETs) 925 lncRNA were identified Pre-GCs...
Blood biochemical indicators play a crucial role in assessing an individual's overall health status and metabolic function. In this study, we measured five blood indicators, including total cholesterol (CHOL), low-density lipoprotein (LDL-CH), triglycerides (TG), high-density (HDL-CH), glucose (BG), as well 19 growth traits of 206 male chickens. By integrating host whole-genome information 16S rRNA sequencing the duodenum, jejunum, ileum, cecum, feces microbiota, assessed contributions...
Objective: Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting highest expression of follicle stimulation hormone receptor (<i>FSHR</i>) will be selected to become a hierarchal follicle. The role lncRNA, miRNA and other non-coding RNA unclear. Methods: In this study, whole transcriptome sequencing SY follicles with different levels <i>FSHR</i> Jining Bairi hens was performed, 30 randomly...
Follicle selection is an important step in the laying process of chicken, which closely related to performance and fecundity hens. mainly depends on regulation follicle-stimulating hormone (FSH) secreted by pituitary gland expression follicle stimulation receptor. To uncover role FSH chicken selection, this study, we analyzed changes mRNA transcriptome profiles FSH-treated granulosa cells from prehierarchical follicles long-read sequencing Oxford Nanopore Technologies (ONT) approach. Among...
The signaling pathway of the wingless-type mouse mammary tumor virus integration site (Wnt) plays an important role in ovarian and follicular development. In our previous study, WNT4 was shown to be involved selection development chicken follicles by upregulating expression follicle-stimulating hormone receptors (FSHR), stimulating proliferation granulosa cells, increasing secretion steroidal hormones. FSH also stimulates WNT4. To further explore molecular mechanism which upregulates...
The development of chicken ovarian follicles involves two key stages primordial follicle recruitment and selection that are tightly regulated by multiple reproductive hormones cytokines. Our previous study revealed an estrogen-stimulated increase in the phosphorylation level serine at position 54 lysine demethylase 1A (LSD1Ser54p) theca cells hierarchical (Post-TCs). In this study, we further found upregulation LSD1Ser54p estrogen was performed glycogen synthase kinase 3 beta (GSK3β) GSK3β...
<title>Abstract</title> Chicken is a valuable model for understanding fundamental biology and vertebrate evolution, as well major source of nutrient-dense lean-protein-enriched food globally. Although it the first non-mammalian amniote genome to be sequenced, chicken still lacks systematic characterization functional variation. Here, through integrating bulk RNA-Seq 7,015 samples, single-cell 127,598 cells 2,869 whole-genome sequences, we presented an atlas regulatory variants across 28...
Abstract The signaling pathway of the wingless-type mouse mammary tumor virus integration site (Wnt) plays an important role in ovarian and follicular development. Wnt4 was shown our previous study to be involved selection development chicken follicles by up-regulating expression follicle stimulating hormone receptors ( FSHR ), proliferation granulosa cells increasing secretion steroidal hormones. To further characterize cis-elements regulating transcription, this we determined critical...