Abstract Background MicroRNAs have been suggested to play important roles in the regulation of gene expression various biological processes. To investigate function miRNAs chicken ovarian development and folliculogenesis, two small RNA libraries constructed from sexually mature (162-day old) immature (42-day ovary tissues Single Comb White Leghorn were sequenced using Illumina deep sequencing. Results In present study, 14,545,100 14,774,864 clean reads obtained (162-d) (42-d) ovaries,...

Ovarian follicle selection is an important process impacting the laying performance and fecundity of hens, regulated by follicle-stimulating hormone (FSH) through binding to its receptor (FSHR). In small yellow (6–8 mm in diameter) with highest expression FSHR will be recruited into preovulatory hierarchy during ovarian development.The study molecular mechanism chicken helpful for identification genes underlying egg traits other poultry species. Herein, transcriptomes follicles differing...

Abstract Background Follicle selection in chickens refers to the process of selecting one follicle from a group small yellow follicles (SY, 6–8 mm diameter) for development into 12–15 hierarchical (usually F6 follicles), which is an important affecting laying performance poultry industry. Although transcriptomic analysis chicken ovarian has been reported, integrated by using both and proteomic approaches still rarely performed. In this study, we compared proteomes transcriptomes SY hens...

The intramuscular fat content (IMF) refers to the amount of within muscles, including sum phospholipids mainly found in cell membranes, triglycerides and cholesterol, is determined both by hyperplasia hypertrophy adipocyte during development pigs. IMF an important economic trait that genetically controlled multiple genes. Laiwu pig indigenous fatty breed distributed North China, characterized excessively higher level (9%~12%), therefore, suitable for identification genes controlling...

In chickens, follicle selection is an important process affecting laying traits, which characterized by the differentiation of granulosa cells and synthesis progesterone from hierarchical follicles. By using Oxford Nanopore Technologies (ONT) approach, we compared transcriptomes between pre-hierarchical (Pre-GCs) follicles (Post-GCs) to identify genes underlying chicken selection. A total 2,436 differentially expressed (DEGs), 3,852 transcripts (DETs) 925 lncRNA were identified Pre-GCs...

Ovarian theca cells play an indispensable role in ovarian follicular development and hormone secretion. miR-26a-5p was reported to be differentially expressed mature immature chicken ovaries our previous study; however, the of regulating follicle function is still unclear. In this study, we demonstrated that expression dynamics TNRC6A mRNA either or follicles showed opposite trend compared with expression. inhibited by directly targeting its 3'-untranslated region cultured cells....

The relationship between stress hyperglycemia and left atrial (LA) abnormality atrioventricular coupling in patients with acute myocardial infarction (AMI) has not been fully explored. This study aimed to assess the additive effect of on LA phasic function investigate interaction AMI via cardiac magnetic resonance imaging (MRI). comprised 120 admission normoglycemia (aNGL), 88 (aHGL), 70 age- sex-matched controls. volume (LAV), ejection fraction (LAEF), reservoir/conduit/contraction strain...

Porcine circovirus type 2 (PCV2), an economically important pathogen, causes postweaning multisystemic wasting syndrome (PMWS) and other diseases collectively known as porcine circovirus-associated disease (PCVAD). Previous studies revealed breed-dependent differences in susceptibility to PCV2; however, the genetic mechanism underlying different resistance PCV2 infection remains largely unknown. In this study, we found that Yorkshire × Landrace (YL) pigs exhibited serious clinical features...

The aberrant expression of long non‑coding RNAs is closely associated with drug resistance in multiple types cancer. Long intergenic RNA 00707 (LINC00707) has previously been reported to be an oncogene able promote lung adenocarcinoma cell proliferation and metastasis. However, its role the progression cisplatin (DDP) non‑small‑cell cancer (NSCLC) requires further elucidation. In present study, LINC00707 microRNA (miR)‑145 levels were measured using reverse transcription‑quantitative PCR...

Gaining insight into the gene expression patterns during ovarian follicle development will benefit improvement of laying performance as well better understanding avian reproductive physiology. To understand molecular regulation development, a systematic profiling miRNAs and mRNAs from ovary at four key stages (at ages 60 days (A), 100 (B), 140 days-not yet eggs (C) days-just starting to lay (D)) were performed. A total 73 differentially expressed (DEMs) (14 for B vs. A, 8 C 51 D C,...

Porcine circovirus-associated disease (PCVAD) is one of the most serious infectious diseases in pigs worldwide. The primary causative agent PCVAD porcine circovirus type 2 (PCV2), which can cause lymphoid depletion and immunosuppression pigs. Our previous study demonstrated that Laiwu (LW) pigs, a Chinese indigenous pig breed, have stronger resistance to PCV2 infection than Yorkshire × Landrace (YL) In this study, we found YL showed more severe lymphocyte apoptosis higher viral load spleen...

Porcine circovirus type 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome (PMWS) and other PCV-associated diseases. According to our previous RNA-sequencing analysis, differences in susceptibility PCV2 infection depended on genetic between Laiwu (LW) Yorkshire × Landrace crossbred (YL) pigs, but cellular microRNA (miRNA) that are differentially expressed LW YL pigs before after remain be determined. In this study, high-throughput sequencing was performed determine...

The myxovirus resistance (Mx) proteins belong to the dynamin superfamily and are important for innate host defence against RNA viruses. In this study, we demonstrate that positive elements present in two promoter regions of −2713 −2565 −688 −431 porcine MX1 gene. Sequencing alignment amplified gene region identified a short interspersed repetitive element (SINE) insertion 275 bp at site −547. At site, allele B (an bp) is dominant Chinese indigenous pig breeds but has workable minor frequency...

In hens, follicle selection is an important process affecting egg laying traits. This study investigated the role of parathyroid hormone-like hormone (PTHLH) in chicken selection, its transcriptional regulation and genetic effects on PTHLH receptor PTH1R were mainly expressed follicles 6-8 mm diameter, exhibits differential expression pattern theca granulosa cells pre- hierarchal follicles. stimulates proliferation follicular cells, StAR CYP11A1 mRNA production progesteron (P4)...

Objective: Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting highest expression of follicle stimulation hormone receptor (<i>FSHR</i>) will be selected to become a hierarchal follicle. The role lncRNA, miRNA and other non-coding RNA unclear. Methods: In this study, whole transcriptome sequencing SY follicles with different levels <i>FSHR</i> Jining Bairi hens was performed, 30 randomly...

The present study aimed to assess the role of long non-coding RNA-urothelial cancer associated 1 (lncRNA-UCA1)/microRNA (miR)-383/vascular endothelial growth factor A (VEGFA) axis in regulating lung adenocarcinoma physiology through vivo and vitro experiments. expression profile lncRNA-UCA1 was analyzed by genome-wide analysis from GSE146459. cell counting Kit-8, colony formation, wound healing transwell assays were performed evaluate effects vitro. In addition, luciferase reporter confirm...

Abstract Background Follicle selection in chickens refers to the process of selecting one follicle from a group small yellow follicles (SY, 6–8 mm diameter) for development into 12–15 hierarchal (usually F6 follicles), which is controlled by sex hormones including follicle-stimulating factor (FSH), estrogen and progesterone. critical impacting egg production chicken, therefore, focus many studies. Phosphorylation important proper function proteins, thus, needs be analyzed proteomic level....

Folate deficiency (FD) during pregnancy can cause fetal intrauterine growth restriction in pigs, of which the skeletal dysplasia is a major manifestation. Factors influencing muscle development are very important formation porcine meat quality trait. However, effect folate on and its molecular mechanisms unknown. The objective this study to determine maternal transcriptome piglets from reciprocal cross, full-sibling Landrace (LR) Chinese local breed Laiwu (LW) pigs were used for cross...