A5056712067- Plant Stress Responses and Tolerance
- Angiogenesis and VEGF in Cancer
- Calcium Carbonate Crystallization and Inhibition
- Pluripotent Stem Cells Research
- Plant nutrient uptake and metabolism
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- RNA modifications and cancer
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Bone Tissue Engineering Materials
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Nicotinic Acetylcholine Receptors Study
- Plant biochemistry and biosynthesis
- Ecology and Conservation Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
- Meat and Animal Product Quality
- CRISPR and Genetic Engineering
- Lung Cancer Treatments and Mutations
- Berberine and alkaloids research
- Alkaloids: synthesis and pharmacology
Shanghai Institute of Materia Medica
2024
Chinese Academy of Sciences
2024
Spanish National Centre for Cardiovascular Research
2017-2024
Southwest Forestry University
2022-2024
Public Health Clinical Center of Chengdu
2024
Guizhou University
2022-2023
Shaoxing University
2022-2023
South China Agricultural University
2022-2023
Tobacco Research Institute
2022-2023
Fourth Affiliated Hospital of Guangxi Medical University
2022
Abstract Appropriate therapeutic modulation of endothelial proliferation and sprouting is essential for the effective inhibition angiogenesis in cancer or its induction cardiovascular disease. The current view that an increase growth factor concentration, resulting mitogenic activity, increases both sprouting. Here, we modulate stimuli different vascular contexts by interfering with function VEGF Notch signalling pathways at high spatiotemporal resolution vivo. Contrary to prevailing view,...
Improved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development disease. Inducible functional genetic mosaics can be extraordinarily useful in the study biological systems; however, this experimental approach is still rarely used vertebrates. This mainly due to technical difficulties assembly large DNA constructs carrying multiple regulatory elements their targeting genome. In addition, mosaic phenotypic analysis,...
Abstract Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of Cre recombinase-dependent deletion genes containing LoxP sites. This system enables conditional genetic modifications because expression and activity recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters time ligand tamoxifen. Since precise recombination event is invisible, methods were developed to report are widely used. However, numerous studies have...
Abstract Third-generation EGFR tyrosine kinase inhibitors (TKIs), exemplified by osimertinib, have demonstrated promising clinical efficacy in the treatment of non-small cell lung cancer (NSCLC). Our previous work has identified ASK120067 as a novel third-generation TKI with remarkable antitumor effects that undergone New Drug Application (NDA) submission China. Despite substantial progress, acquired resistance to EGFR-TKIs remains significant challenge, impeding long-term effectiveness...
Cold stress causes considerable damage to tender tea seedlings. Previous studies have explored changes in the physiological and biochemical factors of response cold stress; however, mechanisms resistance ancient tree plants are unclear. The aim this study was analyze effects 0 °C for 15 days 24 ambient temperature recovery 5 on characteristics two representative old varieties: Dali Siqiu tea. results revealed significant antioxidant, photosynthetic efficiency, indicators stress, with species...
To predict the molecular mechanisms of action Coptis chinensis in treatment Alzheimer’s disease using network pharmacology. The active ingredients and targets were obtained from Traditional Chinese Medicine System Pharmacology Database. Target information for was screened GeneCard OMIM databases. Venn diagram tool used to identify intersecting disease. target entered into STRING database construct a protein-protein interaction network. R language perform Gene ontology Kyoto Encyclopedia...
Potassium (K+) is essential for crop growth. Increasing the K+ content can often directly promote improvement of yield and quality. Heterosis plays an important role in genetic leads to gains. We found that tobacco showed significant heterosis, which highly cultivating varieties with high content. However, mechanism by heterosis occurs leaves not clear. In this study, a comprehensive comparative transcriptome sequencing analysis root samples from hybrid G70 × GDH11 its parental inbred lines...
Pistacia chinensis Bunge (P. chinensis), a dioecious plant species, has been widely found in China. The female P. plants are more important than male agricultural production, as their seeds can serve an ideal feedstock for biodiesel. However, the sex of is hard to distinguish during seedling stage due scarcity available transcriptomic and genomic information. In this work, Illumina paired-end RNA sequencing assay was conducted unravel profiles flower buds. total, 50,925,088 51,470,578 clean...
Lutein can increase the body's skin color and has antioxidant potential. However, how it affects lipid metabolism oxidative stress in chickens remains unknown. In this study, 74-day-old male raised on feed supplemented with lutein had higher hip, back, breast, leg, shin abdominal fat yellowness than control group, livers of group superoxide dismutase glutathione peroxidase lower malondialdehyde activities. To clarify potential regulatory network regulated by lutein, we used RNA-seq...
Nicotine is a unique alkaloid present in tobacco that widely used cigarettes and the agricultural, chemical, pharmaceutical industries. However, research on nicotine mostly limited to its synthesis pathways, only few studies have explored effects of other metabolic pathways precursors. Regulating content can greatly promoting application fields. In this study, we performed global data-independent acquisition proteomics analysis four varieties. Of varieties, one had high three low content. A...
Abstract Background Potassium(K + ) plays a vital role in improving the quality of tobacco leaves. However, how to improve potassium content leaves has always been difficult problem planting. K hybrid is characterized by heterosis, which can leaves, but its underlying molecular genetic mechanisms remain unclear. Results Through two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 weak were screened out. Transcriptome analyses revealed that 80.89% 57.28% differentially...
Heterosis has greatly improved the yield and quality of crops. However, previous studies often focused on improving shoot system, while research root system was neglected. We determined numbers 12 F 1 hybrids, all which showed strong heterosis, indicating that tobacco hybrids have general heterosis. To understand its molecular mechanism, we selected two with GJ (G70 × Jiucaiping No.2) KJ (K326 No.2), their parents for transcriptome analysis. There were 84.22% 90.25% differentially expressed...
Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality worldwide. Many studies have shown that dedicator of cytokinesis 2 (DOCK2) has crucial role as prognostic factor in various cancers. However, the potentiality DOCK2 diagnosis HCC not been fully elucidated. In this work, we aimed to investigate mutation HCC. The Cancer Genome Atlas (TCGA) International Consortium (ICGC) cohorts were utilized identify frequency DOCK2. Then, univariate Cox proportional hazard...
To understand gene function, it is necessary to compare cells carrying the mutated target with normal cells. In most biomedical studies, being compared are in different mutant and control animals and, therefore, do not experience same epigenetic changes tissue microenvironment. The experimental induction of genetic mosaics essential determine a cell-autonomous function model etiology diseases caused by somatic mutations. Current technologies used induce mice lack either accuracy, throughput...
Mollusk shells are characterized by hierarchical aggregation of calcium carbonate and organic matrix, matrix protein is considered as a key active ingredient to understand shell biomineralization. In this study, total 21 proteins, including novel Hcprismatin-14 were identified in the EDTA-soluble prismatic layer mussel Hyriopsis cumingii liquid chromatography tandem mass spectrometry (LC-MS/MS). The full length cDNA was cloned from mantle H. . contains high proportion Gly, Tyr, Arg Asp...
Abstract To understand gene function, it is necessary to compare cells carrying the mutated target with normal cells. In most biomedical studies, being compared are in different mutant and control animals therefore do not experience same epigenetic changes tissue microenvironment. The experimental induction of genetic mosaics essential determine a cell-autonomous function model etiology diseases caused by somatic mutations. Current technologies used induce mice lack either accuracy,...
Abstract Arteries are thought to be formed by the induction of a highly conserved arterial genetic program in subset vessels experiencing an increase pulsatile and oxygenated blood flow. Both VEGF Notch signalling have been shown essential for initial steps specification. Here, we combined inducible mosaics transcriptomics modulate understand function these pathways on cell proliferation, arterial-venous differentiation mobilization. We observed that endothelial cells with high or not...