A5075057949- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Circular RNAs in diseases
- GDF15 and Related Biomarkers
- Bacteriophages and microbial interactions
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
- Pneumonia and Respiratory Infections
- CRISPR and Genetic Engineering
- Plant Pathogens and Fungal Diseases
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Hepatitis B Virus Studies
- Oral and Maxillofacial Pathology
- Entomopathogenic Microorganisms in Pest Control
- Molecular Biology Techniques and Applications
- Kruppel-like factors research
- Insect and Pesticide Research
- dental development and anomalies
- Microbial infections and disease research
- Heart Failure Treatment and Management
- Plant responses to water stress
- Plant Surface Properties and Treatments
Tianjin University of Technology
2025
Shenyang University of Chemical Technology
2024
Dalian Institute of Chemical Physics
2024
Chinese Academy of Sciences
2024
Dalian National Laboratory for Clean Energy
2024
Nanjing Agricultural University
2022-2023
Beijing Forestry University
2022-2023
Chiba University
2023
China Medical University
2021-2022
National Institute of Biological Sciences, Beijing
2014-2021
Abstract Though vascular smooth muscle cell (VSMC) proliferation underlies all cardiovascular hyperplastic disorders, our understanding of the molecular mechanisms responsible for this cellular process is still incomplete. Here we report that SRSF1 (serine/arginine-rich splicing factor 1), an essential factor, promotes VSMC and injury-induced neointima formation. Vascular injury in vivo proliferative stimuli vitro stimulate expression. Mice lacking specifically SMCs develop less intimal...
RNA editing increases transcriptome diversity through post-transcriptional modifications of RNA. Adenosine deaminases that act on (ADARs) catalyze the adenosine-to-inosine (A-to-I) conversion, most common type in higher eukaryotes. Caenorhabditis elegans has two ADARs, ADR-1 and ADR-2, but their functions remain unclear. Here, we profiled editomes C. at different developmental stages wild-type ADAR mutants. We developed a new computational pipeline with “bisulfite-seq-mapping-like” step...
Objective— In this study, we attempted to uncover the functional impact of microRNA-22 (miR-22) and its target gene in smooth muscle cell (SMC) differentiation delineate molecular mechanism involved. Approach Results— miR-22 was found be significantly upregulated during SMC from embryonic stem cells adventitia stem/progenitor cells. Enforced expression by mimic, while knockdown antagomiR, promotes or inhibits cells, respectively. Expectedly, overexpression promoted vivo. Methyl CpG–binding...
The hepatitis B virus (HBV) infects 257 million people worldwide. HBV infection requires establishment and persistence of covalently closed circular (ccc) DNA, a viral episome, in nucleus. Here, we study cccDNA spatial localization the 3D host genome by using chromosome conformation capture-based sequencing analysis fluorescence situ hybridization (FISH). We show that transcriptionally inactive is not randomly distributed Rather, it preferentially accumulated at specialized areas, including...
Because of the higher conservation RSV Fusion (F) protein than glycoprotein (G) across strains and serotypes, majority vaccine candidates targets to viral fusion rather elicit a broader range protective neutralizing antibodies from infection. In this study, we screened two chemically modified mRNA vaccines expressing prefusion stabilized (preF) targeting A2 B subtypes. After immunization, antigen-specific binding antibody, T cell-mediated immune response were evaluated. challenge with live...
This study analyzed the generation rule of streaming potential (SP) considering piezoelectric effect bone under static and dynamic compression. The equation was introduced into SP, an osteon's 3D fluid-structure interaction finite element model with osteocytes developed using COMSOL software. Seven working conditions helped SP. results showed positive negative SP alternately acted on osteocyte loads, about three orders magnitude higher than that loads. Therefore, loads improved osteocytes'...
Oligodontia, which is the congenital absence of six or more permanent teeth, excluding third molars, may contribute to masticatory dysfunction, speech alteration, aesthetic problems and malocclusion. Msh homeobox 1 (MSX1) was first gene identified as causing non-syndromic oligodontia. In this study, we a novel heterozygous non-stop mutation (c.910_911dupTA, p.*304Tyrext*48) in MSX1 Chinese family with autosomal dominant This substitutes stop codon tyrosine residue, potentially adding 48...
To investigate the functional involvements of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) in smooth muscle cell (SMC) differentiation from stem cells, embryonic cells were cultivated on collagen IV-coated plates to allow for SMC differentiation. We found that hnRNPA1 gene and protein expression was upregulated significantly during coexpressed with markers cell-derived SMCs as well 12.5 days mouse embryos. knockdown resulted downregulation transcription factors, while enforced...
Oomycete pathogens secrete numerous effectors to manipulate plant immunity and promote infection. However, relatively few effector types have been well characterized. In this study, members of an FYVE domain-containing protein family that are highly expanded in oomycetes were systematically identified, one secreted protein, PsFYVE1, was selected for further study. PsFYVE1 enhanced Phytophthora capsici infection Nicotiana benthamiana necessary sojae virulence. The domain had PI3P-binding...
Abstract Background Mutations in the basic core promoter (BCP) and its adjacent precore (preC) region HBV genome are common chronic hepatitis B patients. However, patterns of mutation combinations these two regions during infection less understood. This study focused on single base mutations BCP preC multi-mutation observed Methods Total 192 blood samples patients were included. Direct PCR sequencing target was successfully conducted 157 samples. The rest 35 analyzed by clone sequencing....
Abstract The two vasoactive hormones, angiotensin II (ANG II; vasoconstrictive) and atrial natriuretic peptide (ANP; vasodilatory) antagonize the biological actions of each other. ANP acting through receptor-A (NPRA) lowers blood pressure volume. We tested hypothesis that ANG plays critical roles in transcriptional repression Npr1 (encoding NPRA) receptor function. significantly decreased NPRA mRNA protein levels cGMP accumulation cultured mesangial cells attenuated ANP-mediated relaxation...
Drought-resistant plants exhibit strong water retention capability. In this regard, the autotetraploid sour jujube leaves better than diploid leaves. Morphological comparisons and physiological of showed that had thicker leaf cuticles more wax accumulation leaves, which could reduce cuticle permeability improve drought tolerance study, cuticular crystalloids on adaxial abaxial sides young mature were observed in two ploidy types, unique covering a large area may provide an advantage reducing...
Elsholtzia stauntonii Benth. (Lamiaceae) is an economically important ornamental, medicinal and aromatic plant species. To meet the increasing market demand for E. , it necessary to assess genetic diversity within species accelerate process of improvement. Analysis transferability simple sequence repeat (SSR) markers from related or genera a fast economical method evaluate diversity, can ensure availability molecular in crops with limited genomic resources. In this study, cross-genera 497...
Gray mold caused by Botrytis cinerea is a critical disease that results in severe postharvest losses for the apple industry. In recent years, biological control has become an increasingly effective approach controlling diseases fruits. Brassica plants contain abundant natural compounds with known antimicrobial activity against numerous plant pathogens. this study, large-scale screening of 90 mustard cultivars was conducted to evaluate their biofumigation effects B. cinerea. Among these, one...
The mycoparasite Pythium oligandrum is a nonpathogenic oomycete that can boost plant immune responses. Elicitins are microbe-associated molecular patterns (MAMPs) specifically produced by oomycetes activate defense. Here, we identified novel elicitin, PoEli8, from P. exhibits immunity-inducing activity in plants. In vitro-purified PoEli8 induced strong innate responses and enhanced resistance to the pathogen Phytophthora capsici Solanaceae plants, including Nicotiana benthamiana, tomato,...
The effects of whole-genome duplication span multiple levels. Previous study reported that the autotetraploid sour jujube exhibited superior drought tolerance than diploid. However, difference in water transport system between diploids and autotetraploids its mechanism remain unclear. Here, we found number xylem vessels parenchyma cells increased to nearly twice diploid jujube, which may be closely related differences vessel differentiation-related ZjVND7 targets two ploidy types. Although...
In recent years, lignin and its derivatized phenolic compounds have garnered significant attention in the scientific community due to their sustainability. However, reaction for converting lignin-based into other fine chemicals presents several challenges, including stringent conditions, catalyst deactivation, low efficiency. Herein, an efficient synthesis strategy was proposed etherification of with a green methylation reagent dimethyl carbonate under mild constructing chemical barrier wall...
The red turpentine beetle (RTB), Dendroctonus valens LeConte (Coleoptera: Curculionidae), which is native to North America, an invasive wood-boring insect species in China with disastrous consequences. Plant quarantine important method hinder the invasion of this species. In order distinguish RTB from other four closely related bark beetles, Scolytus schevyrewi Semenov, Xyleborus artecomans Schedl, interjectus Blandford and Blastophagus minor Hartig, are frequently intercepted at Chinese...