A5041818639- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Bacterial biofilms and quorum sensing
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Infectious Diseases and Mycology
- Bacteriophages and microbial interactions
- Plant pathogens and resistance mechanisms
- Antimicrobial Peptides and Activities
- Mycotoxins in Agriculture and Food
- Microbial Natural Products and Biosynthesis
- Yeasts and Rust Fungi Studies
- Nematode management and characterization studies
Nanjing Agricultural University
2016-2025
Fujian Electric Power Survey & Design Institute
2024
Chinese Academy of Forestry
2022-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Institute of Plant Protection
2022-2023
Ministry of Education of the People's Republic of China
2022
Plant (United States)
2020-2021
Shenyang Jianzhu University
2017
Beijing Forestry University
2012
A simple microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay was developed for rapid COVID-19 detection.
Plant pathogenic oomycetes, such as Phytophthora sojae, secrete an arsenal of host cytoplasmic effectors to promote infection. We have shown previously that P. sojae PsCRN63 (for crinkling- and necrosis-inducing proteins) induces programmed cell death (PCD) while PsCRN115 blocks PCD in planta; however, they are jointly required for full pathogenesis. Here, we find alone or together might suppress the immune responses Nicotiana benthamiana demonstrate these two interact with catalases from N....
Verticillium wilt caused by soilborne fungus dahliae could significantly reduce cotton yield. Here, we cloned a tomato Ve homologous gene, Gbve1, from an island cultivar that is resistant to wilt. We found the Gbve1 gene was induced V. and phytohormones salicylic acid, jasmonic ethylene, but not abscisic acid. The induction of in quicker stronger than Verticillium-susceptible upland following inoculation. promoter-driving GUS activity exclusively vascular bundles roots stems transgenic...
Tomato yellow leaf curl virus (TYLCV) and its related begomoviruses cause fast-spreading diseases in tomato worldwide. How this induces remains largely unclear. Here we report a noncoding RNA-mediated model to elucidate the molecular mechanisms of TYLCV-tomato interaction disease development. The circular ssDNA genome TYLCV contains intergenic region (IR), which is known mediate viral DNA replication transcription host cells, but has not been reported contribute directly We demonstrate that...
Oomycete pathogens produce a large number of CRN effectors to manipulate plant immune responses and promote infection. However, their functional mechanisms are largely unknown. Here, we identified Phytophthora sojae effector PsCRN108 which contains putative DNA-binding helix-hairpin-helix (HhH) motif acts in the cell nucleus. Silencing gene reduced P. virulence soybean, while expression Nicotiana benthamiana Arabidopsis thaliana enhanced susceptibility capsici. Moreover, could inhibit HSP...
Bacterial–fungal interaction (BFI) has significant implications for the health of host plants. While diffusible antibiotic metabolite-mediated competition in BFI been extensively characterized, impact intercellular contact remains largely elusive. Here, we demonstrate that is a prevalent mode between beneficial soil bacteria and pathogenic filamentous fungi. By generating antibiotics-deficient mutants two common bacteria, Lysobacter enzymogenes Pseudomonas fluorescens , show...
Early and accurate detection of the causal pathogen Phytophthora sojae is crucial for effective prevention control root stem rot seedling damping-off soybean. In present study, a novel isothermal amplification assay was developed detecting P. sojae. This 25-min included two-step approach. First, pair primers, PSYPT-F PSYPT-R were used to amplify specific fragment Ypt1 gene in 20-min recombinase polymerase (RPA) step. Second, lateral flow dipsticks (LFD) detect visualize RPA amplicons within...
Phytophthora megakarya (Pmeg) and palmivora (Ppal) are closely related species causing cacao black pod rot. Although Ppal is a cosmopolitan pathogen, the only known host of economic importance for Pmeg. Pmeg more virulent on than Ppal. We sequenced compared genomes identified virulence-related putative gene models (PGeneM) that may be responsible their differences in specificities virulence. have estimated genome sizes 126.88 151.23 Mb PGeneM numbers 42,036 44,327, respectively. The...
Environmental conditions are key factors in the progression of plant disease epidemics. Light affects outbreak diseases, but underlying molecular mechanisms not well understood. Here, we report that light-harvesting complex II protein, LHCB5, from rice is subject to light-induced phosphorylation during infection by blast fungus Magnaporthe oryzae We demonstrate single-nucleotide polymorphisms (SNPs) LHCB5 promoter control expression which turn correlates with LHCB5. enhances broad-spectrum...
Invasive alien species often have reduced genetic diversity and must adapt to new environments. Given the success of many invasions, this is sometimes called paradox invasion.
Abstract The mirid bug Apolygus lucorum has become a major agricultural pest since the large-scale cultivation of Bt-cotton. It was assumed that A. lucorum, similarly to other phloem sap insects, could secrete saliva contains effector proteins into plant interfaces perturb host cellular processes during feeding. However, secreted effectors are still uncharacterized and unstudied. In this study, 1878 putative were identified from transcriptome which either had homology with published aphid or...
Abstract EDS1 (Enhanced Disease Susceptibility 1) plays a crucial role in both effector‐triggered immunity activation and plant basal defence. However, whether pathogen effectors can target or an EDS1‐related pathway to manipulate is rarely reported. In this study, we identified Phytophthora capsici Avirulence Homolog (Avh) RxLR (Arg‐any amino acid‐Leu‐Arg) effector PcAvh103 that interacts with EDS1. We demonstrated facilitate P. infection required for virulence. Furthermore, genetic...
Many pathogens infect hosts through specific organs, such as Ustilaginoidea virens, which infects rice panicles. Here, we show that a microbe-associated molecular pattern (MAMP), Ser-Thr-rich Glycosyl-phosphatidyl-inositol-anchored protein (SGP1) from U. induces immune responses in leaves but not SGP1 is widely distributed among fungi and acts proteinaceous, thermostable elicitor of BAK1-dependent defense N. benthamiana. Plants specifically recognize 22 amino acid peptide (SGP1 N terminus...
Resistance to Phytophthora sojae (Rps) genes have been widely used in soybean against root and stem rot diseases caused by this oomycete. Among 15 known Rps genes, Rps1k has the most past four decades. Here, we show that products of two distinct but closely linked RxLR effector are detected Rps1k-containing plants, resulting disease resistance. One is Avr1b-1, confers avirulence presence Rps1b. Three lines evidence, including overexpression gene silencing Avr1b-1 stable P. transformants, as...
On the basis of its downy mildew–like morphology, litchi blight pathogen was previously named Peronophythora litchii. Recently, however, it proposed to transfer this Phytophthora clade 4. To better characterize unusual oomycete species and important fruit pathogen, we obtained genome sequence litchii compared those from other species. P. has a small with tightly spaced genes. multilocus phylogenetic analysis, placement in genus is strongly supported. Effector proteins predicted included 245...
Salicylic acid (SA) plays a crucial regulatory role in plant immunity. NPR1 (non-expressor of pathogenesis related-1) is SA receptor and pivotal signaling. However, pathogen effectors which target to promote infection have rarely been reported. Here, we identified Phytophthora capsici effector RxLR48 that associates with NPR1, facilitates P. required for virulence. Furthermore, demonstrated promotes NPR1's nuclear localization inhibits its proteasome-mediated degradation, suggesting...
During plant-pathogenic fungi and host plants interactions, numerous pathogen-derived proteins are secreted resulting in the activation of unfolded protein response (UPR) pathway. For efficient trafficking secretory proteins, including those important disease progression, cytoplasmic coat complex II (COPII) exhibits a multifunctional role whose elucidation remains limited. Here, we discovered that COPII cargo receptor MoErv29 functions as target MoHac1, previously identified transcription...
Tobacco black shank caused by Phytophthora nicotianae is a serious disease in tobacco cultivation. We found that naringenin key factor causes different sensitivity to P. between resistant and susceptible tobacco. The level of basal flavonoids was distinct from Of all with content, showed the best antimicrobial activity against mycelial growth sporangia production vitro. However, very low or no other plant pathogens. induced not only accumulation reactive oxygen species, but also expression...
Plant intracellular nucleotide-binding domain, leucine-rich repeat-containing receptors (NLRs) activate a robust immune response upon detection of pathogen effectors. How NLRs induce downstream defense genes remains poorly understood. The Mediator complex plays central role in transducing signals from gene-specific transcription factors to the machinery for gene transcription/activation. In this study, we demonstrate that MED10b and MED7 mediate jasmonate-dependent repression, coiled-coil...
Yam (Dioscorea spp.) is an important tuber crop consumed globally. However, stable yam production faces challenges from a variety of diseases caused by fungi, nematodes, viruses, and bacteria. Prominent such as anthracnose, leaf spot, wilt, dry rot, crazy root syndrome, currently pose serious threats to yields. These not only result in quality degradation but also cause great economic losses. This review summarizes the damages, symptoms, causal agents, epidemic factors major diseases. It...
Inhibiting pathogen chemotaxis is a promising strategy for reducing disease pressure. However, this currently in the proof-of-concept stage. Here, Phytophthora sojae was used as model, its biflagellated zoospores could sense genistein, soybean root exudate, to navigate host and initiate infection. We identify P. IRK1 (isoflavone-insensitive receptor kinase 1) with PsIRK2 functioning coreceptor that enhances binding affinity of PsIRK1 genistein regulates by phosphorylating G protein α...
MicroRNAs (miRNAs), a group of small noncoding RNAs, may serve as class post-transcriptional regulators in plant immune systems. Nevertheless, little is known about their roles response to the oomycete pathogens. To identify miRNAs involved soybean Phytophthora sojae , we examined expressional patterns upon infection by P. microarray analysis three cultivars: Williams (susceptible), Conrad (quantitative resistance), and 82 (qualitative resistance). Expression number was significantly altered...