A5102864959- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Insect and Pesticide Research
- interferon and immune responses
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- Synthesis and Biological Evaluation
- Viral-associated cancers and disorders
- Insect symbiosis and bacterial influences
- Viral Infectious Diseases and Gene Expression in Insects
- Entomopathogenic Microorganisms in Pest Control
- Immune Response and Inflammation
- Computational Drug Discovery Methods
- Receptor Mechanisms and Signaling
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
- HIV/AIDS drug development and treatment
- Viral gastroenteritis research and epidemiology
- RNA regulation and disease
Nanjing Agricultural University
2006-2024
Wuhan University
2015-2024
Wuhan Institute of Virology
2024
Sanya University
2023
Renmin Hospital of Wuhan University
2023
Chongqing University of Posts and Telecommunications
2021
Jiangsu University
2018-2019
Tianjin People's Hospital
2019
Nantong University
2018
Tianjin Hospital
2014
Extensive planting of crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) has suppressed some major pests, reduced insecticide sprays, enhanced pest control by natural enemies, and increased grower profits. However, rapid evolution resistance in pests is reducing these benefits. Better understanding genetic basis Bt urgently needed monitor, delay, counter resistance. We discovered that a point mutation previously unknown tetraspanin...
Abstract Insect ryanodine receptors (RyRs) are the targets of diamide insecticides. Two point mutations G4946E and I4790M (numbering according to Plutella xylostella , PxRyR) in transmembrane domain insect RyRs associated with resistance have so far been identified three lepidopteran pests, P. Tuta absoluta Chilo suppressalis . In this study, we one known RyR target site (I4790M) a field‐collected population Spodoptera exigua The WF S. exhibited 154 fold chlorantraniliprole when compared...
Abstract The mirid bug Apolygus lucorum has become a major agricultural pest since the large-scale cultivation of Bt-cotton. It was assumed that A. lucorum, similarly to other phloem sap insects, could secrete saliva contains effector proteins into plant interfaces perturb host cellular processes during feeding. However, secreted effectors are still uncharacterized and unstudied. In this study, 1878 putative were identified from transcriptome which either had homology with published aphid or...
Kaposi’s sarcoma-associated herpesvirus (KSHV) is a double-stranded DNA virus with the capacity to establish life-long latent infection. During infection, viral genome persists as circular episome that associates cellular histones and exists nonintegrated minichromosome in nucleus of infected cells. Chromatin structure epigenetic programming are required for proper control gene expression stable maintenance DNA. However, there still limited knowledge regarding how host regulates chromatin...
Transgenic crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce benefits this approach. Recessive mutations in extracellular domain toxin-binding cadherin proteins that confer Bt toxin Cry1Ac disrupting binding reported previously three major lepidopteran including cotton bollworm, Helicoverpa armigera. Here we report a novel allele from bollworm with deletion intracellular is genetically...
Viral infection activates the transcription factors NF-κB and IRF3, which contribute to induction of type I interferons (IFNs) cellular antiviral responses. Protein kinases play a critical role in various signaling pathways by phosphorylating their substrates. Here, we identified dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 2 (DYRK2) as negative regulator virus-triggered IFN induction. DYRK2 inhibited IFNs promoted K48-linked ubiquitination degradation TANK-binding 1 (TBK1)...
Abstract BACKGROUND Plutella xylostella (L.) is a worldwide pest of crucifer crops. Chlorfenapyr has been used extensively to manage several insect pests in vegetables, including P. . In this study, 48 field populations were collected from 11 geographic locations China 2008 2017 for resistance monitoring chlorfenapyr. Inheritance and synergism chlorfenapyr characterized field‐derived population. RESULTS Compared with the susceptible SZ strain, 33 39 Eastern or developed low levels (<...
Abstract BACKGROUND The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides some advantages over other reverse genetic techniques to investigate the causal relationship between insecticide resistance phenotype and candidate gene. Several studies published date point nicotinic acetylcholine receptor (nAChR) α6 subunit strongly associated with spinosyns in insects, including Plutella xylostella . However, verification of P. nAChRα6 has not yet been achieved...
Transgenic crops have revolutionized insect pest control, but evolution of resistance by pests threatens their continued success. The primary strategy for combating to producing insecticidal proteins from Bacillus thuringiensis (Bt) uses refuges non-Bt host plants allow survival susceptible insects. prevailing paradigm is that delay rare and recessively inherited. However, we discovered countered Bt cotton was neither nor recessive. In a 15-year field study the bollworm, frequency mutation...
The SZ-F strain of Plutella xylostella (L.) (Lepidoptera: Plutellidae) was derived from a field-collected (SZ) by 20 generations continuous selection with fipronil. resulted in 300-fold increase resistance to fipronil, and 3.5- 6.5-fold cross-resistance dieldrin endosulfan, respectively, the compared unselected SZ strain. Analysis detoxification enzyme activities bioassay synergists indicated that metabolic mechanisms are not important fipronil is most likely attributed target site...
The SZ-F strain of Plutella xylostella (L.) (Lepidoptera: Plutellidae) was derived from a field-collected (SZ) by 20 generations continuous selection with fipronil. resulted in 300-fold increase resistance to fipronil, and 3.5- 6.5-fold cross-resistance dieldrin endosulfan, respectively, the compared unselected SZ strain. Analysis detoxification enzyme activities bioassay synergists indicated that metabolic mechanisms are not important fipronil is most likely attributed target site...
Internet of Things and Smart Grid are very popular topics recently. In such a context, this paper makes analysis on the application requirements IoT in combined with basic functions IoT. Under framework Grid, structure have been proposed tentatively. is an intelligent network based technology, database communication technology so on. Its will significantly improve power system's stability security, as well protect environment meet sustainable development requirements. Moreover, level system...
The overexpression of metabolic enzymes constitutes a crucial mechanism for insects to detoxify xenobiotics and pesticides. A flavin-containing monooxygenase gene (PxFMO2) from Plutella xylostella P450 (SeCYP9A186, F116V mutant allele) Spodoptera exigua have been reported be involved in insecticide resistance. In this study, we aim utilize transgenic technology validate their vivo detoxification functions xylostella.
A field-collected strain (HF) of Plutella xylostella (L.) showed 420-fold resistance to fipronil compared with a susceptible laboratory (Roth). The HF-R strain, derived from the HF by 25 generations successive selection in laboratory, developed 2,200-fold relative Roth strain. F(1) progeny reciprocal crosses between and 640-fold (R♀ × S♂) 1,380-fold (R♂ S♀) fipronil, indicating is inherited as an incompletely dominant trait. Analysis backcross (F1♂ suggests that controlled one major locus....
KIF15, the vertebrate kinesin‐12, is best known as a mitotic motor protein, but continues to be expressed in neurons. Like KIF11 (the kinesin‐5), KIF15 interacts with microtubules axon limit their sliding relative one another. Unlike KIF11, also regulates interactions between and actin filaments at sites of axonal branch formation growth cones. Our original work on these motors was done cultured rat neurons, we are now using zebrafish extend studies an vivo model. We previously studied kif15...
Enterovirus 71 (EV71) plays an important role in hand-foot-and-mouth disease. In this study, a series of diarylhydrazide analogues was synthesized, and the systematic exploration SAR led to potent enterovirus inhibitors, which compound 15 exhibits significant improvements inhibition potency with EC50 value 0.02 μM against EV71. It is very interesting that class diarylhydrazides activities human enteroviruses at picomolar level, including EV71 Coxsackieviruses B1 (CVB1), CVB2, CVB3, CVB4,...
Previous studies reported the association between single nucleotide polymorphism (SNP) of IL15 receptor alpha (IL15RA) gene with susceptibility to ossification posterior longitudinal ligament spine (OPLL). However, results were still in controversy. Therefore, purpose present study was investigate SNPs IL15RA OPLL a Chinese Han population.A total 235 patients and 250 age-matched healthy controls recruited. All subjects genotyped using PCR (polymerase chain reaction)-based invader assay. A...
Halolactones with an isobenzofuran-1(3<italic>H</italic>)-one core structure have been developed as efficient non-nucleoside reverse transcriptase inhibitors (NNRTIs). The best compound<bold>13a</bold>showed excellent potency against WT HIV-1 a low EC<sub>50</sub>value of 0.45 μM.
Reactivation of Kaposi's sarcoma-associated herpesvirus (KSHV) is important for persistent infection in the host as well viral oncogenesis. The replication and transcription activator (RTA) encoded by KSHV ORF50 plays a central role switch from latency to lytic replication. Given that RTA transcriptional expression sufficient activate complete replication, must possess an elaborate mechanism regulating its protein abundance. Previous studies have demonstrated could be degraded through...