A5070931202- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Food Chemistry and Fat Analysis
- Insect Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Climate Change Communication and Perception
- CRISPR and Genetic Engineering
- Climate change impacts on agriculture
- Genetically Modified Organisms Research
- Species Distribution and Climate Change
- Plant Pathogens and Resistance
- Crop Yield and Soil Fertility
- Studies on Chitinases and Chitosanases
- Plant Stress Responses and Tolerance
- Rural Development and Agriculture
- Plant Parasitism and Resistance
- Biochemical and biochemical processes
- Agricultural Economics and Policy
- Bacillus and Francisella bacterial research
- Food Supply Chain Traceability
- Insect behavior and control techniques
- Agriculture Sustainability and Environmental Impact
Agriculture and Food
2020-2022
International Life Sciences Institute
2020
Beltsville Agricultural Research Center
2012-2017
Plant (United States)
2012-2017
National Institute of Food and Agriculture
2016-2017
Agricultural Research Service
2011-2015
Pennsylvania State University
2008-2012
United States Department of Agriculture
2011
Theobroma cacao (cacao) is cultivated in tropical climates and exposed to drought stress. The impact of the endophytic fungus Trichoderma hamatum isolate DIS 219b on cacao's response was studied. Colonization by delayed drought-induced changes stomatal conductance, net photosynthesis, green fluorescence emissions. altered expression 19 expressed sequence tags (ESTs) (seven leaves 17 roots with some overlap) detected using quantitative real-time reverse transcription PCR. Roots tended respond...
Endophytic Trichoderma isolates collected in tropical environments were evaluated for biocontrol activity against Phytophthora capsici hot pepper (Capsicum annuum). Six tested parasitic and antimicrobial P. endophytic induced resistance capabilities pepper. Isolates DIS 70a, 219b, 376f parasites, while 259j, 320c, metabolites inhibited capsici. All six colonized roots but inefficient stem colonizers. defense-related expressed sequence tags (EST) 32-day-old peppers. delayed disease...
The basidiomycete Moniliophthora roreri is the causal agent of Frosty pod rot (FPR) disease cacao (Theobroma cacao), source chocolate, and FPR one most destructive diseases this important perennial crop in Americas. This hemibiotroph infects only pods has an extended biotrophic phase lasting up to sixty days, culminating plant necrosis sporulation fungus without formation a basidiocarp. We sequenced assembled 52.3 Mb into 3,298 contigs that represent M. genome. Of 17,920 predicted open...
Phytophthora megakarya (Pmeg) and palmivora (Ppal) are closely related species causing cacao black pod rot. Although Ppal is a cosmopolitan pathogen, the only known host of economic importance for Pmeg. Pmeg more virulent on than Ppal. We sequenced compared genomes identified virulence-related putative gene models (PGeneM) that may be responsible their differences in specificities virulence. have estimated genome sizes 126.88 151.23 Mb PGeneM numbers 42,036 44,327, respectively. The...
Frosty pod rot (FPR) of Theobroma cacao (cacao) is caused by the hemibiotrophic fungus Moniliophthora roreri. Cacao clones tolerant to FPR are being planted throughout Central America. To determine whether M. roreri shows a differential molecular response during successful infections clones, we collected field-infected pods at all stages symptomatology for two highly susceptible (Pound-7 and CATIE-1000) three (UF-273, CATIE-R7 CATIE-R4). Metabolite analysis was carried out on Pound-7,...
The aim of this study was to determine whether endophytic Bacillus cereus isolates from agronomic crops possessed genes for the nonhaemolytic enterotoxin (Nhe) and haemolysin BL (HBL) and, therefore, have potential cause diarrhoeal illness in humans. PCR followed by sequencing confirmed presence nheA, nheB, nheC, hblA, hblC, hblD B. cereus. All nhe were detected 59% cereus, while all hbl 44%. six 41% isolates. Enterotoxin not 15% Reverse transcriptase real-time that could express pure...
Frosty pod rot (FPR) is a devastating cacao disease caused by the basidiomycete Moniliophthora roreri (Aime & Phillips-Mora, 1). The confined to 13 countries in Central and South America constitutes permanent threat for cultivation worldwide. In July 2012, FPR was detected Alto Beni, La Paz Department, Bolivia where 85% of production produced approximately 3000 small-holders. Typical symptoms signs were observed villages San Luis, Villa Prado y 3 de Mayo (Area III) Litoral, Antonio Porvenir...
Frosty pod rot ( FPR ), caused by Moniliophthora roreri , is responsible for significant losses in Theobroma cacao . Due to limited options management, biological control methods using Trichoderma are being studied. Combinations of three formulations and two isolates were studied between May 2009 April 2011. The 0·3 mL L −1 the surfactant BreakThru 100 SL BT a mixture 1% w/v Sure‐Jell (source pectin) potato dextrose broth PDB ) PP an invert oil emulsion 50% v/v corn oil/2·5% lecithin/0·5%...
An understanding of the tolerance mechanisms Theobroma cacao used against Moniliophthora roreri, causal agent frosty pod rot, is important for generation stable disease-tolerant clones. A comparative view was obtained transcript populations infected pods from two susceptible and tolerant clones using RNA sequence (RNA-Seq) analysis. total 3009 transcripts showed differential expression among KEGG (Kyoto Encyclopedia Genes Genomes) pathway analysis differentially expressed genes indicated...
Communication theory suggests that interactive dialog rather than information transmission is necessary for climate change action, especially complex systems like agriculture. Climate analogs-locations whose current similar to a target location's future climate-have garnered recent interest as transmitting more relatable information; however, they have unexplored potential in facilitating meaningful dialogs, and whether the way analogs are developed could make difference. We context-specific...
Abstract Confined field trials (CFT) of genetically engineered (GE) crops are used to generate data inform environmental risk assessments (ERA). ERAs required by regulatory authorities before novel GE can be released for cultivation. The transportability CFT assessment in countries other than those where the was conducted has been discussed previously an analysis showing that primary difference between locations potentially impacting trial outcomes is physical environment, particularly...