A5082446213- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Plant Pathogens and Fungal Diseases
- Microbial Community Ecology and Physiology
- Plant pathogens and resistance mechanisms
- Plant Disease Resistance and Genetics
- Plant Disease Management Techniques
- Plant tissue culture and regeneration
- Soil Carbon and Nitrogen Dynamics
- Mycorrhizal Fungi and Plant Interactions
- Plant nutrient uptake and metabolism
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Plant Pathogens and Resistance
- Plant Virus Research Studies
- Entomopathogenic Microorganisms in Pest Control
- Plant and Fungal Interactions Research
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Smart Agriculture and AI
- Ovarian cancer diagnosis and treatment
- Insect symbiosis and bacterial influences
Beltsville Agricultural Research Center
2015-2024
Agricultural Research Service
2014-2023
United States Department of Agriculture
2005-2023
The University of Sydney
2017-2018
Sustainable Energy Systems (United Kingdom)
2016
Oil Crops Research Institute
2015
Chinese Academy of Agricultural Sciences
2015
Center for Biologics Evaluation and Research
2012
University of Nottingham
2012
Animal and Plant Health Inspection Service
2010
The grand challenge confronting agriculture is the development of technologies for sustainable intensification crop production systems to feed estimated future human population 9 10 billion. It thought that must be increased by 60 100% year 2050 meet these nutritional needs. Crop yield more food higher content are needed at same time have a diminished impact on environment. Prior agricultural was through substantial use fertilizer, pesticides, and irrigation all significant environmental...
We investigated the effects of two different plant species (corn and soybean) three soil types on microbial community structure in rhizosphere. Our working hypothesis was that rhizosphere effect would be strongest fast-growing aerobic heterotrophs, while there little or no oligotrophic other slow-growing microorganisms. Culturable bacteria fungi had larger population densities than bulk soil. Communities were characterized by fatty acid analysis substrate utilization assays for fungi. Fatty...
The egl gene of Pseudomonas solanacearum was cloned on a cosmid and expressed in Escherichia coli. Restriction endonuclease mapping, transposon mutagenesis, subclone analysis showed that the located 2.7-kilobase XhoI-SalI P. DNA fragment. Immunoabsorption experiments sodium dodecyl sulfate-polyacrylamide gel electrophoretic encodes 43-kilodalton endoglucanase is major excreted solanacearum. In E. coli, appeared to be from its own promoter, but product restricted cytoplasm. mutagenized with...
Endophytic Trichoderma isolates collected in tropical environments were evaluated for biocontrol activity against Phytophthora capsici hot pepper (Capsicum annuum). Six tested parasitic and antimicrobial P. endophytic induced resistance capabilities pepper. Isolates DIS 70a, 219b, 376f parasites, while 259j, 320c, metabolites inhibited capsici. All six colonized roots but inefficient stem colonizers. defense-related expressed sequence tags (EST) 32-day-old peppers. delayed disease...
Feeding nutrition-dense food to future world populations presents agriculture with enormous challenges as estimates indicate that crop production must much double. Crop cannot be increased meet this challenge simply by increasing land acreage or using past agricultural intensification methods. Food doubled in the through substantial use of synthetic fertilizer, pesticides, and irrigation, all at significant environmental cost. Future will require next-generation systems decreased reliance on...
Colonization of the spermosphere and rhizosphere by plant-beneficial bacteria is limited competition with indigenous soil microbes for resources such as reduced carbon compounds. A study microbial community around germinating seeds was undertaken a necessary first step in understanding between introduced community. Two types, Galestown gravely loamy sand soil, pH 5.8 0.6% humic material, Hatborough 4.5 3.2% were compared. Five seed corn, cucumber, radish, soybean, sunflower used. Microbial...
Alternative tactics for improving phosphorus nutrition in crop production are needed China and elsewhere, as the overapplication of phosphatic fertilizers can adversely impact agricultural sustainability. Penicillium oxalicum P4 Aspergillus niger P85 were isolated from a calcareous soil that had been exposed to excessive application fertilizer decades. Each isolate excreted number organic acids into, acidified, solubilized synthetic broth containing insoluble tricalcium phosphate or rock...
Abstract The agricultural community is confronted with dual challenges; increasing production of nutritionally dense food and decreasing the impacts these crop systems on land, water, climate. Control plant pathogens will figure prominently in meeting challenges as diseases cause significant yield economic losses to crops responsible for feeding a large portion world population. New approaches technologies enhance sustainability and, importantly, disease control need be developed adopted. By...
A major endopolygalacturonase excreted by Pseudomonas solanacearum was purified to greater than 95% homogeneity and shown have an isoelectric point of 9.0 a subunit molecular mass 52 kilodaltons (kDa). The gene encoding this enzyme (pglA) isolated from genomic library P. DNA based on its expression in Escherichia coli be contained 1.8-kilobase fragment. identity the pglA product 52-kDa polygalacturonase demonstrated immunoadsorption focusing experiments. cloned apparently expressed own...
Abstract Background Pseudomonas syringae pv. tagetis ( Pstag ) causes apical chlorosis on sunflower and various other plants of the Asteraceae family. Whole genome sequencing strain EB037 transposon-mutant derivatives, no longer capable causing chlorosis, was conducted to improve understanding molecular basis disease caused by this pathogen. Results A tripartite pathogenicity island (TPI) for a Type III secretion system (T3SS) with complete hrp-hrc gene cluster conserved effector locus...
Abstract The bacterium Burkholderia cepacia (strain Bc-2) and the fungus Trichoderma virens Gl-3) were investigated for activity against nematode Meloidogyne incognita. Culture filtrates from Bc-2 Gl-3 contained extracellular factors that inhibited egg hatch second-stage juvenile (J2) mobility. Size fractionation results lack of detectable chitinase or protease activities culture suggested inhibitory in vitro assays non-enzymic. Tomato root explant cultures M. incognita treated with T.virens...
There is a pressing need for redesigning agriculture to achieve sustainability and utilizing modern genetic tools, including engineering, add nutritional value crops the benefit of diverse human population. Collectively, plant foods contain most minerals, macronutrients (calories), micronutrients essential nutrition. These also range bioactive compounds that can play important roles in prevention chronic diseases heart disease, cancer, stroke, diabetes, Alzheimer's, cataracts, age-related...
Gene expression profiles of the biological control strain Pseudomonas protegens Pf-5 inhabiting pea seed surfaces were revealed using a whole-genome oligonucleotide microarray. We identified genes expressed by under two global regulators (GacA and RpoS) known to influence secondary metabolism. Transcript levels 897 genes, including many with unknown functions as well those for biofilm formation, cyclic diguanylate (c-di-GMP) signalling, iron homeostasis metabolism, influenced one or both...
Sclerotinia sclerotiorum causes serious yield losses in crops the People’s Republic of China. Two formulations oilseed rape seed containing bacterium Bacillus subtilis Tu-100 were evaluated for suppression this pathogen field trials conducted at two independent locations. The pellet formulation significantly reduced disease (incidence and index) increased plant dry mass, while wrap incidence mass both Mean per 120 plants with isolate was greater than appropriate controls, but only one Both...