- Nematode management and characterization studies
- Entomopathogenic Microorganisms in Pest Control
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Management Techniques
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Insect Pest Control Strategies
- Weed Control and Herbicide Applications
- Agronomic Practices and Intercropping Systems
- Allelopathy and phytotoxic interactions
- Biofuel production and bioconversion
- Plant and fungal interactions
- Lichen and fungal ecology
- Soybean genetics and cultivation
- Botany and Plant Ecology Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Agricultural Economics and Policy
- Fungal Biology and Applications
- Plant Disease Resistance and Genetics
- Insect and Pesticide Research
- Yeasts and Rust Fungi Studies
- Fern and Epiphyte Biology
Beltsville Agricultural Research Center
2010-2023
United States Department of Agriculture
1997-2020
Agricultural Research Service
2006-2020
University of Missouri
2019
Southeastern Fruit and Tree Nut Research Laboratory
2013
Virginia Cooperative Extension
2013
University of Georgia
1985-2013
Animal and Plant Health Inspection Service
2010
U.S. National Arboretum
2006
Greatbatch (United States)
1990
Pseudomonas fluorescens isolates Clinto 1R, Wayne and Wood which produce the antibiotic 2,4-diacetylphloroglucinol (DAPG), can suppress soilborne diseases promote plant growth. Consequently, these beneficial bacterial were tested on watermelon plants for suppression of Meloidogyne incognita (root-knot nematode: RKN) Fusarium oxysporum f. sp. niveum (Fon). In a greenhouse trial, 1R root dip suppressed numbers RKN eggs per gram 'Charleston Gray' by 28.9%. However, in studies focused 'Sugar...
Abstract The bacterium Burkholderia cepacia (strain Bc-2) and the fungus Trichoderma virens Gl-3) were investigated for activity against nematode Meloidogyne incognita. Culture filtrates from Bc-2 Gl-3 contained extracellular factors that inhibited egg hatch second-stage juvenile (J2) mobility. Size fractionation results lack of detectable chitinase or protease activities culture suggested inhibitory in vitro assays non-enzymic. Tomato root explant cultures M. incognita treated with T.virens...
Abstract Fungi were isolated from soybean cyst nematode (SCN, Heterodera glycines) eggs collected in China, and 253 fungal isolates assayed for production of compounds active against SCN root-knot (RKN, Meloidogyne incognita). Fungal grown 3 7 days potato dextrose broth (PDB), the culture broths sterile-filtered to remove biomass, filtrates placed into 24-well plates test effects on egg hatch juvenile motility. incognita ranged 2 121% PDB controls H. glycines 15 224%. Activities harvested...
Abstract An isolate of the fungus Chaetomium globosum produced culture broths that inhibited in vitro egg hatch and juvenile mobility root-knot nematode (Meloidogyne incognita) soybean cyst (Heterodera glycines). Extraction bioassay-directed fractionation broth filtrate determined flavipin, a low molecular weight compound, was metabolite responsible for most nematode-antagonistic activity. Synthesis flavipin permitted evaluation compound as suppressor populations on plants glasshouse...
Abstract Restrictions on the use of conventional nematicides have increased need for new methods managing plant‐parasitic nematodes. Consequently, nematode‐antagonistic microbes, and active compounds produced by such organisms, are being explored as potential additions to management practices. Programs in this area at USDA Agricultural Research Service investigate applied biocontrol agents, naturally occurring beneficial soil microbes natural compounds. Specific research topics include plant...
ABSTRACT Cyclic hydroxamic acids are secondary metabolites found in the family Poaceae and have been implicated allelopathy of rye (Secale cereale). The toxicity these compounds against plant-parasitic nematodes is unknown. DIBOA (2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4H)-one), DIMBOA (2,4-hydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)-one), their degradation products BOA (benzoxazolin-2(3H)-one) MBOA (6-methoxy-benzoxazolin-2(3H)-one) were screened vitro Meloidogyne incognita second-stage...
Clove oil, derived from the plant Syzygium aromaticum (L.) Merr. & Perry, is active against various organisms, and was prepared in a soy lecithin/detergent formulation to determine concentrations root-knot nematode Meloidogyne incognita (Kofoid White) Chitwood.In microwell assays, mean effective clove oil concentration that reduced egg hatch by 50% (EC(50)) 0.097% (v/v) oil; EC(50) for second-stage juvenile (J2) viability 0.145% (compared with carrier control treatments). Volatiles 5.0%...
Species of Cephalotaxus (the plum yews) produce nematotoxic compounds unknown identity. Consequently, bioassay-guided fractionation was employed to identify the compound(s) in fortunei twigs and leaves with activity against plant-parasitic nematodes.A crude alkaloid extract, particularly drupacine, responsible for much nematotoxicity. The ED50 drupacine Bursaphelenchus xylophilus 27.1 µg mL⁻¹, Meloidogyne incognita it 76.3 mL⁻¹. Immersion M. eggs 1.0 mg mL⁻¹ extract highest tested...