Abstract Background Root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. cause an estimated $100 billion annual loss worldwide. For successful establishment of the root-knot nematode in its host plant, it causes dramatic morphological and physiological changes cells. The expression some genes is altered by as establishes feeding site. Results We examined soybean ( Glycine max ) galls formed roots nematode, Meloidogyne incognita , 12 days 10 weeks...

Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long explants derived the meristamatic region 2-3 year old offshoots cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 150 mg l-1 2,4-dichlorophenoxy acetic acid (2,4-D) incubated in dark for 6 weeks initiate callus. Callogenesis was obtained all 2,4-D concentrations tested; however, callus growth most significant...

Abstract Genetically engineered tomato hairy root lines with the ability to synthesize glycine betaine were established by introducing aldehyde dehydrogenase gene (BADH-1) from sorghum plants. The showed a mikimopine activity, confirming transformation pRi plasmid. stable integration of BADH into genome was confirmed Southern blotting analysis. expression mRNA could be detected in roots Northern osmotic potential (Ψ s) transgenic sufficient allow water uptake. accumulation 1H-NMR contributed...

Real-time prediction of video source traffic is an important step in many network management tasks such as dynamic bandwidth allocation and end-to-end quality-of-service (QoS) control strategies. In this paper, adaptive model for MPEG-coded developed. A novel technology used, first developed the signal processing community, called sparse basis selection. It based on selecting a small subset inputs (basis) from among large dictionary possible inputs. new selection algorithm that efficiently...

This investigation examines responses of protoplasts in a systematic and quantitative way to the various electrical treatments used achieve electrofusion their individual cumulative effect on protoplast viability. Mesophyll cell suspension from two species same genera, Nicotiana tabacum N. rustica var brasilia were these experiments. Optimal frequencies for alignment tobacco between 500 kilohertz 2 megahertz at 100 volts per centimeter. Variations frequency voltage alternating current (AC)...

Numerous studies and clinical investigations have been carried out on the metabolism of poly unsaturated fatty acids (PUFAs) in general omega-3 particular over past two decades. They are involved a number stress responses plants, as illustrated by genomic <i>A</i><i>rabidopsis</i><i> </i><i>t</i><i>haliana</i>. Alignment sequences phylogenetic analysis followed motif elicitation provided ample evidences to show that resistance/tolerance genes identical which may contribute same all or most...

Somatic embryos derived from walnut (Juglans regia L.) ovule tissues were evaluated to determine whether they of zygotic or maternal origin. Molecular markers used permit evaluation at an early stage, before whole plant development. developed potentially apomictic `Sunland' and `Cisco' tissue isolated bagged putatively unpollinated flowers. Phosphoglucomutase (PGM) isozyme analysis showed that all these embryos, except one each cultivar, carry the same zymotype as tissue. However,...

The availability of high-throughput sequencing technologies increased our understanding different genomes. However, the genomes all living organisms still have many unidentified coding sequences. number missing small open reading frames (sORFs) is due to length threshold used in most gene identification tools, which true genic and, more importantly and surprisingly, intergenic regions. Scanning cucumber genome regions revealed 420 723 sORF. We excluded 3850 sORF with similarities annotated...

Transformed plants of Egyptian clover (berseem clover, Torifolium alexandrinum L.) were obtained from hairy roots induced by infection with Agrobacterium rhizogenes strain DC-AR2 (harboring mikimopine-type pRi1724). Among established 16 root lines showing growth variations on MS medium containing 0.5 mg l-1 NAA, two moderate or slow proliferation regenerating shoots spontaneously. The presence T-DNA pRi1724 in the genome regenerated was confirmed DNA gel blot analysis. repeatedly formed but...

Many members of the Plumbaginaceae are important flower crops wherein propagation is hindered by poor seed germination. Micropropagation via organogenesis commercially practiced for certain Limonium species. However, somatic embryogenesis was not reported until recently L. bellidifolium Durmort. The induction from cotyledon explants in a modified Murashige and Skoog (MS) medium examined four other this family, aureum O. Kuntze, latifolium sinuatum Mill., Armeria maritima Willd. Induction...