A5022710072- Nematode management and characterization studies
- Genetics, Aging, and Longevity in Model Organisms
- Legume Nitrogen Fixing Symbiosis
- Entomopathogenic Microorganisms in Pest Control
- Parasite Biology and Host Interactions
- Circadian rhythm and melatonin
- Plant Parasitism and Resistance
- Helminth infection and control
- Insect Pest Control Strategies
- Cholesterol and Lipid Metabolism
- Neurobiology and Insect Physiology Research
- Hormonal Regulation and Hypertension
- Algal biology and biofuel production
- Plant Disease Management Techniques
- Steroid Chemistry and Biochemistry
- Insects and Parasite Interactions
- Studies on Chitinases and Chitosanases
- Soybean genetics and cultivation
- Agronomic Practices and Intercropping Systems
- Birth, Development, and Health
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Animal Nutrition and Physiology
- Plant and animal studies
- Parasites and Host Interactions
Agricultural Research Service
2004-2019
United States Department of Agriculture
1992-2019
Beltsville Agricultural Research Center
1983-2018
Yonsei University
2008-2009
University of Maryland, College Park
1981-1994
Hungarian Academy of Sciences
1994
A low-input sustainable agricultural system for the production of staked, fresh-market field tomatoes ( Lycopersicon esculentum Mill.) is described. The uses winter annual cover crops to fix N, recycle leftover nutrients, produce biomass, and prevent soil erosion throughout spring. Yields tomato plants grown in hairy vetch Vicia villosa Roth), crimson clover Trifolium incarnatum L.), rye Secale cereale L.) plus mulches were higher than those conventional black polyethylene (BP) mulch 2 3...
Caenorhabditis elegans excretes a dauer pheromone or daumone composed of ascarylose and fatty acid side chain, the perception which enables worms to enter state for long-term survival in an adverse environment. During course elucidation biosynthetic pathway DHS-28 DAF-22 are involved peroxisomal beta-oxidation VLCFAs (very long-chain acids), we sought investigate physiological consequences deficiency biosynthesis C. elegans. Our results revealed that two mutants, dhs-28(tm2581)...
Abstract The bacterium Burkholderia cepacia (strain Bc-2) and the fungus Trichoderma virens Gl-3) were investigated for activity against nematode Meloidogyne incognita. Culture filtrates from Bc-2 Gl-3 contained extracellular factors that inhibited egg hatch second-stage juvenile (J2) mobility. Size fractionation results lack of detectable chitinase or protease activities culture suggested inhibitory in vitro assays non-enzymic. Tomato root explant cultures M. incognita treated with T.virens...
Abstract Caenorhabditis elegans was cultured in semi‐defined medium containing yeast extract, soy peptone, glucose, hemoglobin, Tween 80, and sitosterol. Monoglycosylceramides were chromatographically purified from nematode extracts. Their structures elucidated with mass spectrometry, nuclear magnetic resonance spectroscopy, analysis of methanolysis products the parent cerebrosides. The glycosylceramides unusual that only long‐chain sphingoid base detected an iso ‐branched compound a C‐4...
Caenorhabditis elegans requires sterol, usually supplied as cholesterol, but this is enzymatically modified, and different sterols can substitute. Sterol deprivation decreased brood size adult growth in the first generation, completely, reversibly, arrested larvae second. After one generation of sterol deprivation, 10 ng/ml cholesterol allowed delayed laying a few eggs, full required 300 ng/ml. C. synthesizes two unusual 4alpha-methyl (4MSs), each 4MS supported only limited sole sterol....
Summary This review focuses on recent research developments in the past three years concerning ecdysteroid biochemistry helminths and annelids, with emphasis results reported at IXth Ecdysone Workshop Paris, France, September 1989. During years, most of this area has been concentrated nematodes, several species which occurrence ecdysteroids demonstrated. Interesting biological effects exogenously applied have discovered meiotic reinitiation oocytes dog heartworm, Dirofilaria immitis,...
In 2006, a cyst nematode was discovered in tare dirt at potato (Solanum tuberosum) processing facility eastern Idaho. The found during routine survey conducted jointly by the Idaho State Department of Agriculture and USDA Animal Plant Health Inspection Service through Cooperative Agricultural Pest Survey program. Extensive additional sampling from two suspect fields led to identification same 45-acre (18.2-ha) field located northern Bingham County. morphology cysts second-stage juveniles...
Abstract An isolate of the fungus Chaetomium globosum produced culture broths that inhibited in vitro egg hatch and juvenile mobility root-knot nematode (Meloidogyne incognita) soybean cyst (Heterodera glycines). Extraction bioassay-directed fractionation broth filtrate determined flavipin, a low molecular weight compound, was metabolite responsible for most nematode-antagonistic activity. Synthesis flavipin permitted evaluation compound as suppressor populations on plants glasshouse...
Clove oil, derived from the plant Syzygium aromaticum (L.) Merr. & Perry, is active against various organisms, and was prepared in a soy lecithin/detergent formulation to determine concentrations root-knot nematode Meloidogyne incognita (Kofoid White) Chitwood.In microwell assays, mean effective clove oil concentration that reduced egg hatch by 50% (EC(50)) 0.097% (v/v) oil; EC(50) for second-stage juvenile (J2) viability 0.145% (compared with carrier control treatments). Volatiles 5.0%...
Abstract The metabolism of various dietary sterols and the effects an azasteroid on sitosterol in free‐living nematode Caenorhabditis elegans was investigated. major unesterified C. media supplemented with sitosterol, cholesterol or desmosterol included 7‐dehydrocholesterol (66.5%, 40.5%, 31.2%, respectively), (6.7%, 52.3%, 26.9%), lathosterol (4.4%, 3.6%, 1.7%) 4α‐methylcholest‐8(14)‐en‐3β‐ol (4.2%, 2.1%, 3.8%). Esterified sterols, representing less than 20% total were somewhat similar...
An unusual population of cyst nematode was found in soils collected from a Powell Butte, OR field with cropping history including potato, wheat, other crops, and significant weed presence. These nematodes could not be placed certainty into any known species exhibited some unique morphological features specimens. Compared Globodera pallida, the body length slightly longer second-stage juvenile stylet shorter. In individuals, J2 knob height greater tail annules were more prominent than G....
Species of Cephalotaxus (the plum yews) produce nematotoxic compounds unknown identity. Consequently, bioassay-guided fractionation was employed to identify the compound(s) in fortunei twigs and leaves with activity against plant-parasitic nematodes.A crude alkaloid extract, particularly drupacine, responsible for much nematotoxicity. The ED50 drupacine Bursaphelenchus xylophilus 27.1 µg mL⁻¹, Meloidogyne incognita it 76.3 mL⁻¹. Immersion M. eggs 1.0 mg mL⁻¹ extract highest tested...
Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both sedentary endoparasitic nematodes, their development parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- retinol-binding (FAR) proteins nematode-specific lipid carrier used for nutrient acquisition as well suppression plant defenses. In this study, we three novel FAR genes Ha-far-1 (KU877266), Ha-far-2 (KU877267), Hf-far-1 (KU877268). were cloned avenae, encoding...
The backbone structure of ginsenosides, active ingredients Panax ginseng, is similar with that sterol, especially cholesterol. Caenorhabditis elegans (C. elegans) one free living nematodes and well-established animal model for biochemical genetic studies. C. cannot synthesize de novo cholesterol, although cholesterol essential requirement its growth development. In the present study, we investigated effects ginseng total saponins (GTS) on average brood size, growth, development, worm life...