We report an effective, novel self-assembled single-wall carbon nanotube (SWNT) complex with oligonucleotide and demonstrate its feasibility in recognizing detecting specific DNA sequences a single step homogeneous solution. The key component of this is the hairpin-structured fluorescent that allows SWNT to function as both "nanoscaffold" for "nanoquencher" fluorophore. Given functionality, represents new class universal fluorescence quenchers are substantially different from organic should...

Make it a double: Complementary probes with two pyrene labels were engineered for the amplification of target DNA sequence. In stem-closed conformation in absence target, moieties separated. The propagated hybridization chain reactions to bring on neighboring close together form fluorescent excimers (see picture).

Epithelial cell adhesion molecule (EpCAM) is overexpressed in most solid cancers and an ideal antigen for clinical applications cancer diagnosis, prognosis, imaging, therapy. Currently, of the EpCAM-based diagnostic, prognostic, therapeutic strategies rely on anti-EpCAM antibody. However, use EpCAM antibody restricted due to its large size instability. In this study, we have successfully identified DNA aptamers that selectively bind human recombinant protein. The can specifically recognize a...

The World Health Organization has declared the outbreak of a novel coronavirus (SARS-CoV-2 or 2019-nCoV) as global pandemic. However, mechanisms behind infection are not yet fully understood, nor there any targeted treatments vaccines. In this study, we identified high-binding-affinity aptamers targeting SARS-CoV-2 RBD, using an ACE2 competition-based aptamer selection strategy and machine learning screening algorithm. Kd values optimized CoV2-RBD-1C CoV2-RBD-4C against RBD were 5.8 nM 19.9...

Cocaine cracked: The gel–sol transition of an enzyme-caged hydrogel has been efficiently controlled by target binding events, which trigger release the enzyme to take part in its catalytic role for signal amplification (see picture). As low as 20 ng cocaine can be visually detected within 10 min without any aid sophisticated instrumentation. Visual detection is increasingly attractive method many fields because both qualitative and semiquantitative assessment performed real time advanced or...

An aptamer switch probe (ASP) linking chlorin e6 (Ce6), a photosensitizer molecule, to the surface of gold nanorods (AuNRs) was used target cancer cells for photodynamic therapy (PDT) and photothermal (PTT). In presence cells, ASP changes conformation drive Ce6 away from surface, thereby producing singlet oxygen PDT upon light irradiation. Since each AuNR is modified with many ASP-Ce6 molecules, AuNR-ASP-Ce6 conjugate yields enhanced binding therapeutic effect by added ability carry...

Portable devices with the advantages of rapid, on-site, user-friendly, and cost-effective assessment are widely applied in daily life. However, only a limited number quantitative portable commercially available, among which personal glucose meter (PGM) is most successful example has been used. PGMs can detect blood as unique target. Here we describe novel design that combines glucoamylase-trapped aptamer-cross-linked hydrogel PGM for detection non-glucose targets. Upon target introduction,...

A novel aptamer-based molecular probe design employing intramolecular signal transduction is demonstrated. The composed of three elements: an aptamer, a short, partially cDNA sequence, and PEG linker conjugating the aptamer with short DNA strand. We have termed this "aptamer switch probe", or ASP. ASP utilizes both fluorophore quencher which are respectively modified at two termini In absence target molecule, will hybridize keeping in close proximity, thus switching off fluorescence....

In this paper, we report the assembly of single-walled carbon nanotubes (SWNTs) and single-stranded DNA to develop a new class fluorescent biosensors which are able probe recognize biomolecular interactions in homogeneous format. This novel sensing platform consists structure formed by interaction SWNTs dye-labeled oligonucleotides such that proximity nanotube dye effectively quenches fluorescence absence target. Conversely, very importantly, competitive binding target or protein with for...

Abstract Point‐of‐care testing (POCT) with the advantages of speed, simplicity, portability, and low cost is critical for measurement analytes in a variety environments where access to laboratory infrastructure lacking. While qualitative POCTs are widely available, quantitative present significant challenges. Here we describe novel method that integrates an Au core/Pt shell nanoparticle (Au@PtNP) encapsulated target‐responsive hydrogel volumetric bar‐chart chip (V‐Chip) POCT. Upon target...

Abstract Immunotherapy has revolutionized cancer treatment, but its efficacy is severely hindered by the lack of effective predictors. Herein, we developed a homogeneous, low‐volume, efficient, and sensitive exosomal programmed death‐ligand 1 (PD‐L1, type transmembrane protein) quantitation method for diagnosis immunotherapy response prediction (HOLMES‐Exo PD‐L1 ). The combines newly evolved aptamer that efficiently binds to with less hindrance antigen glycosylation than antibody,...

A disposable, equipment-free, versatile point-of-care testing platform, microfluidic distance readout sweet hydrogel integrated paper-based analytical device (μDiSH-PAD), was developed for portable quantitative detection of different types targets. The platform relies on a target-responsive aptamer cross-linked target recognition, cascade enzymatic reactions signal amplification, and analytic devices (μPADs) visual distance-based readout. "sweet" with trapped glucoamylase (GA) synthesized...

Heterogeneity among individual molecules and cells has posed significant challenges to traditional bulk assays, due the assumption of average behavior, which would lose important biological information in heterogeneity result a misleading interpretation. Single molecule/cell analysis become an emerging field biomedical research for insights into between large populations at high resolution. Compared with ensemble method, single explores on time trajectories, conformational states,...

Tumor-derived exosomal proteins have emerged as promising biomarkers for cancer diagnosis, but the quantitation accuracy is hindered by large numbers of normal cell-derived exosomes. Herein, we developed a dual-target-specific aptamer recognition activated in situ connection system on exosome membrane combined with droplet digital PCR (ddPCR) (TRACER) tumor-derived PD-L1 (Exo-PD-L1 ). Leveraging high binding affinity aptamers, excellent selectivity dual-aptamer recognition, and sensitivity...

A simple microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay was developed for rapid COVID-19 detection.

Recently, lysosome targeting chimeras (LYTACs) have emerged as a promising technology that expands the scope of targeted protein degradation to extracellular targets. However, preparation by conjugation antibody and trivalent N-acetylgalactosamine (tri-GalNAc) is complex time-consuming process. The large uncertainty in number position molecular weights result low internalization efficiency. To circumvent these problems, we developed first aptamer-based LYTAC (Apt-LYTAC) realize...

We have designed a novel photodynamic therapy (PDT) agent using protein binding aptamer, photosensitizer, and single-walled carbon nanotube (SWNT). The PDT is based on covalently linking photosensitizer with an aptamer then wrapping onto the surface of SWNTs, such that can only be activated by light upon target binding. chosen human α-thrombin linked it Chlorin e6 (Ce6), which second generation photosensitizer. Our results showed SWNTs are great quenchers to singlet oxygen (SOG). In presence...

This study demonstrates the use of aptamer-conjugated graphene oxide as an affinity extraction and detection platform for analytes from complex biological media. We have shown that cocaine adenosine can be selectively enriched plasma samples direct mass spectrometric readouts obtained without a matrix with greatly improved signal-to-noise ratios. Aptamer-conjugated has clear advantages in target enrichment generating highly efficient ionization molecules spectrometry. These results...

Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples. By using the quenching nature of oxide multiple fluorophores, this can distinguish highly similar sequences and detect them simultaneously.

Abstract Simultaneous targeted cancer imaging, therapy and real‐time therapeutic monitoring can prevent over‐ or undertreatment. This work describes the design of a multifunctional nanomicelle for recognition precise near‐infrared (NIR) therapy. The encapsulates new pH‐activatable fluorescent probe robust NIR photosensitizer, R16FP, is functionalized with newly screened cancer‐specific aptamer targeting viable cells. light up lysosomes imaging. Upon irradiation, R16FP‐mediated generation...

Noninvasive and accurate measurement of intracellular temperature is great significance in biology medicine. This paper describes a safe, stable, nano-thermometer based on an l-DNA molecular beacon (l-MB), dual-labeled hairpin oligonucleotide built from the optical isomer naturally occurring d-DNA. Relying temperature-responsive structure FRET signaling mechanism MBs, fluorescence l-MBs quenched below melting enhanced with increasing temperature. Because excellent reversibility tunable...

We have developed a photoresponsive DNA-cross-linked hydrogel that can be photoregulated by two wavelengths with reversible sol−gel conversion. This photoinduced conversion further untilized for precisely controllable encapsulation and release of multiple loads. Specifically, photosensitive azobenzene moieties are incorporated into DNA strands as cross-linkers, such their hybridization to complementary DNAs (cDNAs) responds differently different light. On the basis rheology variation...

Because of the severe health risks associated with lead pollution, rapid, sensitive, and portable detection low levels Pb2+ in biological environmental samples is great importance. In this work, a Pb2+-responsive hydrogel was prepared using DNAzyme its substrate as cross-linker for portable, quantitative Pb2+. Gold nanoparticles (AuNPs) were first encapsulated an indicator colorimetric analysis. absence lead, inactive, maintains gel form. contrast, presence activates to cleave substrate,...

Lighting the way with DNA: Molecular beacon micelle flares (MBMFs), based on self-assembly of diacyllipid–molecular-beacon conjugates (L-MBs; see figure), have been developed for combined mRNA detection and gene therapy. These MBMFs were shown to inhibit a model in vitro decrease viability cancer cells culture. The hybridization between nucleic acid strand its complementary sequence, one strongest most specific molecular recognition events,1 has greatly facilitated development disease...