Nanotechnology has allowed the construction of various nanostructures for applications, including biomedicine. However, a simple target-specific, economical, and biocompatible drug delivery platform with high maximum tolerated doses is still in demand. Here, we report aptamer-tethered DNA nanotrains (aptNTrs) as carriers targeted transport cancer therapy. Long aptNTrs were self-assembled from only two short upon initiation by modified aptamers, which worked like locomotives guiding toward...

An aptamer switch probe (ASP) linking chlorin e6 (Ce6), a photosensitizer molecule, to the surface of gold nanorods (AuNRs) was used target cancer cells for photodynamic therapy (PDT) and photothermal (PTT). In presence cells, ASP changes conformation drive Ce6 away from surface, thereby producing singlet oxygen PDT upon light irradiation. Since each AuNR is modified with many ASP-Ce6 molecules, AuNR-ASP-Ce6 conjugate yields enhanced binding therapeutic effect by added ability carry...

This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers be detached the surface by specific target binding. exposes to enzymatic cleavage releases new cycle. Cycling of targets leads significant signal amplification low LOD.

Abstract Selective protein degradation platforms have opened novel avenues in therapeutic development and biological inquiry. Antibody‐based lysosome‐targeting chimeras (LYTACs) emerged as a promising technology that extends the scope of targeted to extracellular targets. Aptamers offer an advantageous alternative owing their potential for modification manipulation toward multivalent state. In this study, chemically engineered platform aptamer‐based LYTACs (AptLYTACs) is established either...

Bone morphogenetic protein (BMP) signaling plays a crucial role in maintaining the pluripotency of mouse embryonic stem cells (ESCs) and has negative effects on ESC neural differentiation. However, it remains unclear when how BMP executes those different functions during commitment. Here, we show that BMP4-sensitive window exists Cells at this specific period correspond to egg cylinder stage epiblast can be maintained as ESC-derived (ESD-EpiSCs), which have same characteristics EpiSCs...

Abstract Breast cancer affects one in eight women the United States, with a mortality rate that is second only to lung cancer. Although chemotherapy widely used breast treatment, its side effects remain challenge. One way address this problem through drug delivery by internalization of cell‐type‐specific probes. nucleic acid aptamers are excellent probes for molecular recognition, few studies have demonstrated can be internalized into living cells. Therefore, herein we report development...

The double helix is a ubiquitous feature of RNA molecules and provides target for nucleases involved in maturation decay. Escherichia coli ribonuclease III participates decay pathways by site-specifically cleaving double-helical structures cellular viral RNAs. site cleavage can determine functional activity half-life specified part local tertiary structure elements such as internal loops. involvement base pair sequence determining sites unclear, because RNase efficiently degrade polymeric...

Escherlchla coll ribonuclease III, purified to homogeneity from an overexpresslng bacterial strain, exhibits a high catalytic efficiency and thermostable processing activity in vitro. The RNase Ill-catalyzed cleavage of 47 nucleotide substrate (R1.1 RNA), based on the bacteriophage T7 R1.1 signal, follows saturation kinetics, with Km 0.26 μM, kcat, 7.7 mln.−1 (37°C, In buffer containing 250 mM potassium glutamate and10 MgCl2. Mn2+ Co2+ can support enzymatic RNA canonical site, both metal...

A mutational approach was employed to identify sequence and structural elements in a ribonuclease III processing signal that are important for vitro enzymatic cleavage reactivity selectivity. The substrate analyzed the bacteriophage T7 R1.1 signal, 60 nucleotide irregular RNA hairpin exhibiting an upper lower dsRNA stem, separated by asymmetric internal loop which contains scissile phosphodiester bond. Altering length of either or segment dose not change site RNase cleavage. However,...

Due to the lack of known therapeutic targets for triple-negative breast cancer (TNBC), chemotherapy is only available pharmacological treatment. Pirarubicin (tetrahydropyranyl Adriamycin, THP) most commonly used anthracycline agent. However, TNBC has a high recurrence rate after chemotherapy, and mechanisms chemoresistance are not entirely understood. To study mechanisms, we first screened compounds on pirarubicin-resistant cell line (MDA-MB-231R) derived from MDA-MB-231. The drug resistance...

The altered faecal metabolites and microbiota might be involved in the development of breast cancer. We aimed to investigate effect differential on proliferative activity cancer cells.We collected samples from 14 patients healthy subjects. Untargeted metabolomics analysis, short-chain fatty acid (SCFA) targeted 16S rDNA sequencing was performed. gut metabolite composition changed significantly. Levels norvaline, glucuronate galacturonate were lower group than Control (p < 0.05)....

Chemotherapy is one of the standard strategies for treatment breast cancer. Adriamycin (Dox) a first‐line chemotherapy agent However, gastrointestinal reactions, myocardial toxicity and other side effects caused by Dox due to its un‐specific cytotoxicity limit clinical effect. To address this need, aptamer has been regarded as an ideal target molecular carrier. In present study, we selected 5TR1 that can specifically bind MUC1 protein which important tumor biomarker, well potential in...

The generation of functional retinal pigment epithelium (RPE) is great therapeutic interest to the field regenerative medicine and may provide possible cures for degenerative diseases, including age-related macular degeneration (AMD). Although RPE cells can be produced from either embryonic stem or induced pluripotent cells, direct cell reprogramming driven by lineage-determining transcription factors provides an immediate route their generation. By monitoring a human specific Best1::GFP...

Introduction: Interleukin-41 (IL-41), also known as Metrnl, is a multifunctional adipokine recognized for its neurotrophic and anti-inflammatory properties that play significant role in diseases such sepsis chronic obstructive pulmonary disease. Despite crucial biological functions, the mechanisms by which IL-41 mitigates lipopolysaccharide (LPS)-induced acute lung injury (ALI) are not well understood. This study aimed to elucidate protective effects of against LPS-induced inflammation...

Abstract Background : The incidence rate of breast cancer ranks highest in both China and the United States. Understanding associations between genetic polymorphisms clinicopathological features may ultimately result improvements prevention, early detection, treatment.The purpose present study was to evaluate seven tagging single nucleotide (tSNPs) risk assessed by tumor pathological characteristics body mass index (BMI). Methods Seven tSNPs four susceptibility genes were analyzed 734...

Abstract Objective The purpose of the present study was to evaluate associations between seven tagging single nucleotide polymorphisms (tSNPs) and risk breast cancer assessed by tumor pathological characteristics body mass index (BMI). Methods Seven tSNPs four susceptibility genes were analyzed in 734 Chinese women with 672 age-matched healthy controls, then association clinicopathological characteristics, BMI, molecular subtype, TNM staging lymph node status, determined. Results Two tSNPs,...

Abstract Objective The purpose of the present study was to evaluate associations between seven tagging single nucleotide polymorphisms (tSNPs) and risk breast cancer assessed by tumor pathological characteristics body mass index (BMI). Methods Seven tSNPs four susceptibility genes were analyzed in 734 Chinese women with 672 age-matched healthy controls, then association clinicopathological characteristics, BMI, molecular subtype, TNM staging lymph node status, determined. Results Two tSNPs,...