Using cell-based aptamer selection, we have developed a strategy to use the differences at molecular level between any two types of cells for identification signatures on surface targeted cells. A group aptamers been generated specific recognition leukemia The selected can bind target with an equilibrium dissociation constant ( K d ) in nanomolar-to-picomolar range. selection process is simple, fast, straightforward, and reproducible, and, most importantly, be done without prior knowledge...

Disease biomarkers play critical roles in the management of various pathological conditions diseases. This involves diagnosing diseases, predicting disease progression and monitoring efficacy treatment modalities. While efforts to identify specific using a variety technologies has increased number or augmented information about them, effective use disease-specific is still scarce. Here, we report that high expression protein tyrosine kinase 7 (PTK7), transmembrane receptor kinase-like...

A novel aptamer-based molecular probe design employing intramolecular signal transduction is demonstrated. The composed of three elements: an aptamer, a short, partially cDNA sequence, and PEG linker conjugating the aptamer with short DNA strand. We have termed this "aptamer switch probe", or ASP. ASP utilizes both fluorophore quencher which are respectively modified at two termini In absence target molecule, will hybridize keeping in close proximity, thus switching off fluorescence....

The identification of tumor related cell membrane protein targets is important in understanding progression, the development new diagnostic tools, and potentially for identifying therapeutic targets. Here we present a novel strategy proteins that are altered their expression levels diseased using specific aptamers. Using an intact viable B-cell Burkitt's lymphoma line (Ramos cells) as target, have selected aptamers recognize with high affinity. Among showed different recognition patterns...

To cut a long story short. Single-stranded DNA aptamers that can recognize cancer cells specifically were optimized and modified. The modifications gave rise to much smaller in size (see scheme) more stable fetal bovine serum. This stability makes it possible apply vivo applications, such as diagnosis therapy. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2007/z600532_s.pdf or from author. Please note: publisher not responsible...

Long-term survival still eludes most patients with leukemia and non-Hodgkin's lymphoma. No approved therapies target the hallmark of B cell, its mIgM, also known as B-cell receptor (BCR). Aptamers are small oligonucleotides that can specifically bind to a wide range molecules offer some advantages over antibodies therapeutic agents. Here, we report rational engineering aptamer TD05 into multimeric forms reactive BCR may be useful in biomedical applications. Systematic truncation coupled...

We report on a new strategy for identifying highly specific aptamers against predetermined epitope of target. Termed "ligand-guided selection" (LIGS), this method uniquely exploits the selection step, core SELEX (Systematic Evolution Exponential enrichment). LIGS uses naturally occurring stronger and bivalent binder, an antibody (Ab) interacting with its cognate antigen to outcompete from partially enriched pool, as strategy. demonstrate hypothesis by utilizing Ab binding membrane-bound...

Aptamers are synthetic oligonucleotides that bind to their specific receptors with high specificity, offering immense potential for the development of molecular tools. Using recently introduced Ligand-Guided Selection (LIGS), a variant Systematic Evolution Ligands by Exponential Enrichment (SELEX) we previously identified anti-CD19 and anti-CD20 aptamers specificity affinity. Given expression levels, B-cell markers CD19 CD20 widely utilized in diagnosis B-cell-related malignancies autoimmune...

We describe the synthesis of C-5 indole-tagged pyrimidine and C-8 purine nucleoside phosphoramidites their incorporation into double-stranded DNA 15 base pairs in length. Of 23 sequence modifications tested, two induced duplex to adopt a Z-like left-handed conformation under physiological salt conditions, bypassing specific sequences typically required for Z-DNA structure. The impact these varied with linker type: flexible propyl linkers exhibited distinct effects compared rigid propargyl...

Protein kinase Cs are a family of serine and threonine kinases that mediate wide variety cellular signalling processes such as cell growth, differentiation, apoptosis tumor development. We have selected high-affinity DNA aptamers for PKCdelta by capillary electrophoresis based SELEX (systematic evolution ligands exponential enrichment, CE-SELEX). demonstrated fluorescently tagged PB9 aptamer can specifically recognize under in vitro conditions. The Kd the aptamer-protein binding is 122 nM....

Photodynamic therapy for tumors. This paper describes the application of a molecular construct photosensitizer and an aptamer phototherapeutically targeting tumor cells. Coupled with advantages cell-SELEX in generating multiple effective aptamers diseased cell recognition, we will be able to develop highly efficient photosensitizer-based therapeutic reagents clinical applications. Supporting information this article is available on WWW under...

Abstract Nucleic acid aptamers (NAAs) are short synthetic DNA or RNA molecules that specifically fold into distinct three‐dimensional structures able to recognize a target. While NAAs show unprecedented promise in variety of applications, including sensing, therapeutics and diagnostics, one major limitation involves the lack stability towards omnipresent nucleases. Therefore, we herein report systematic truncation incorporation 2’‐O‐methyl bases aptamer, which results increased without...

To discover DNA ligands against a predetermined receptor protein complex, we introduce comprehensive version of ligand-guided selection (LIGS). LIGS is, itself, variant systematic evolution by exponential enrichment (SELEX). Herein, have optimized to identify higher affinity aptamers with high specificity. In addition, demonstrate the expandability performing specific aptamer elution at 25°C, utilizing multiple monoclonal antibodies (mAbs) cultured cells and primary obtained from human...

Molecular mediation of singlet oxygen generation is demonstrated based on a newly engineered aptamer probe. Both ATP and human α-thrombin aptamers were to testify this design, both showed that the production can be triggered quantitatively mediated by presence target molecules. Moreover, photosensitizer switch (PAS) probes excellent selectivity toward their targets. These results suggest PAS serve as smart photodynamic therapy agent.

Here we are reporting, for the first time, a ligand-guided selection (LIGS) experiment using an artificially expanded genetic information system (AEGIS) to successfully identify AEGIS-DNA aptamer against T cell receptor-CD3ε expressed on Jurkat.E6 cells. Thus, have effectively combined enhanced diversity of AEGIS DNA library with LIGS develop superior screening platform discover aptamers. Libraries molecules from highly diversified building blocks will provide better ligands due more...

Recently, immunotherapeutic modalities with engineered cells and monoclonal antibodies have been effective in treating several malignancies. Nucleic acid aptamers can serve as alternative molecules to design agents high functional diversity. Here we report a synthetic prototype consisting of DNA that activate the T cell receptor cluster differentiation 3 (TCR-CD3) complex cultured cells. We show activation potential is similar antibody (mAb) against TCR-CD3, suggesting for developing...