A5014580842- RNA Research and Splicing
- Drug Transport and Resistance Mechanisms
- Pancreatic and Hepatic Oncology Research
- Nuclear Structure and Function
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Fibroblast Growth Factor Research
- Reproductive Biology and Fertility
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Sperm and Testicular Function
- Parasites and Host Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Pneumocystis jirovecii pneumonia detection and treatment
- Ferroptosis and cancer prognosis
- Parasitic Infections and Diagnostics
- Protein Kinase Regulation and GTPase Signaling
- Biochemical Analysis and Sensing Techniques
- Renal and related cancers
- Wnt/β-catenin signaling in development and cancer
- Sphingolipid Metabolism and Signaling
- Regulation of Appetite and Obesity
- Mast cells and histamine
Cornell University
2022-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2017-2024
University of Buenos Aires
2017-2024
Weill Cornell Medicine
2022-2024
New York Hospital Queens
2023
Presbyterian Hospital
2023
NewYork–Presbyterian Hospital
2023
Instituto de Química y Fisicoquímica Biológicas
2017-2019
Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society
2019
Universidad Nacional de La Plata
2019
Abstract Exportin-1 (XPO1), the main soluble nuclear export receptor in eukaryotic cells, is frequently overexpressed diffuse large B-cell lymphoma (DLBCL). A selective XPO1 inhibitor, selinexor, received approval as single agent for relapsed or refractory (R/R) DLBCL. Elucidating mechanisms by which overexpression supports cancer cells could facilitate further clinical development of inhibitors. We uncovered here that increases tolerance to genotoxic stress, leading a poor response...
Aptamers are synthetic oligonucleotides that bind to their specific receptors with high specificity, offering immense potential for the development of molecular tools. Using recently introduced Ligand-Guided Selection (LIGS), a variant Systematic Evolution Ligands by Exponential Enrichment (SELEX) we previously identified anti-CD19 and anti-CD20 aptamers specificity affinity. Given expression levels, B-cell markers CD19 CD20 widely utilized in diagnosis B-cell-related malignancies autoimmune...
Peripheral T cell lymphomas (PTCLs) comprise heterogeneous malignancies with limited therapeutic options. To uncover targetable vulnerabilities, we generate a collection of PTCL patient-derived tumor xenografts (PDXs) retaining histomorphology and molecular donor-tumor features over serial xenografting. PDX demonstrates remarkable heterogeneity, complex intratumor architecture, stepwise trajectories mimicking primary evolutions. Combining functional transcriptional stratification...
Is extracellular cAMP involved in the regulation of signalling pathways bovine sperm capacitation?Extracellular induces capacitation through activation different that involve phospholipase C (PLC), PKC/ERK1-2 and an increase Ca2+ levels, as well soluble AC cAMP/protein kinase A (PKA) signalling.In order to fertilize oocyte, ejaculated spermatozoa must undergo a series changes female reproductive tract, known capacitation. This correlates with number membrane metabolic modifications include...
Cestodes are a diverse group of parasites, some them being agents neglected diseases. In cestodes, little is known about the functional properties G protein coupled receptors (GPCRs) which have proved to be highly druggable targets in other organisms. Notably, serotoninergic G-protein (5-HT GPCRs) play major roles key functions like movement, development and reproduction parasites.Three 5-HT GPCRs from Echinococcus granulosus Mesocestoides corti were cloned, sequenced, bioinformatically...
Pancreatic cancer is one of the most lethal types tumors with no effective therapy available; currently third leading cause in developed countries; and predicted to become second deadliest United States by 2030. Due marginal benefits current standard chemotherapy, identification new therapeutic targets greatly required. Considering that cAMP pathway commonly activated pancreatic ductal adenocarcinoma (PDAC) its premalignant lesions, we aim investigate multidrug resistance-associated protein...
Abstract Recent findings show that MRP4 is critical for pancreatic ductal adenocarcinoma (PDAC) cell proliferation. Nevertheless, the significance of protein levels and function in PDAC progression still unclear. The aim this study was to determine role tumor aggressiveness. Bioinformatic studies revealed samples higher transcript compared normal adjacent tissue circulating cells express than primary tumors. Also, high are typical high-grade lines associate with an epithelial-mesenchymal...
ABSTRACT Mammalian spermatozoa must undergo biochemical and structural changes to acquire the capacity for fertilization, in a process known as capacitation. Activation of PKA enzymes is essential capacitation, thus cAMP levels are tightly regulated during this process. Previously, we demonstrated that bovine extrude through multidrug resistance-associated protein 4 (MRP4, also ABCC4), which regulates intracellular nucleotide provides extracellular space. Here, report presence functional...
Abstract About 40% of diffuse large B-cell lymphoma (DLBCL) pts do not respond to immunochemotherapy. In human and murine models, we others have shown that the microenvironment (LME) changes with disease progression treatment resistance. This cell-LME co-evolutionary process results in expansion treatment-resistant cells as well reduced infiltration T reprogramming CAFs TAMs into immune suppressive entities. Our analysis relapse or refractory (RR)-DLBCLs showed only an depleted LME but also...
The xenobiotic transporter ABCC4 /MRP4 is highly expressed in pancreatic ductal adenocarcinoma (PDAC) and correlates with a more aggressive phenotype metastatic propensity. Here, we show that promotes epithelial-mesenchymal transition (EMT) PDAC, hallmark process involving the acquisition of mesenchymal traits by epithelial cells, enhanced cell motility, chemoresistance. Modulation levels PANC-1 BxPC-3 lines resulted dysregulation genes present EMT signature. Bioinformatic analysis on...
The β-blocker propranolol (PROP) has been proposed as a repurposed treatment for breast cancer. similarity of action between β-agonists and antagonists found on cells encouraged us to compare PROP isoproterenol (ISO, agonist) signaling pathways human cell line. Cell proliferation was measured by counting DNA-synthesis. adhesion the that remained adhered plastic after different treatments. Changes in actin cytoskeleton were observed fluorescence staining Western Blot. ISO caused diminution an...
Intracellular cAMP (i-cAMP) levels play an important role in acute myeloid leukemia (AML) cell proliferation and differentiation. Its are the result of production, degradation, exclusion. We have previously described histamine H2 receptors MRP4/ABCC4 as two potential targets for AML therapy. Acting through receptors, increases production/synthesis, while is responsible exclusion this cyclic nucleotide. In study, we show that treatment induces expression, augmenting efflux, histamine,...
Despite its importance in the regulation of growth and differentiation processes a variety organisms, mechanism synthesis degradation cAMP (cyclic AMP) has not yet been described Giardia lamblia In this work, we measured significant quantities trophozoites G. incubated vitro later detected how it increases during first hours encystation, then returns to basal levels at 24 h. Through an analysis genome lamblia, found sequences three putative enzymes - one phosphodiesterase (gPDE) two...
The aim of this work was to study the effect a high sodium (HS) diet on blood pressure and renal function in male adult rats that have been treated with dual Endothelin receptor antagonist (ERA) during their early postnatal period (day 1 20 life). Male Sprague-Dawley were divided four groups: CNS: control normosodic diet; ERANS: ERA-treated CHS: ERAHS: HS diet. Systolic (SBP) recorded before after 24-hour metabolic cage studies performed. AQP2 α-ENac expressions measured by western blot real...
<p>Supplementary Figures and Tables</p>
<div>Abstract<p>Exportin-1 (XPO1), the main soluble nuclear export receptor in eukaryotic cells, is frequently overexpressed diffuse large B-cell lymphoma (DLBCL). A selective XPO1 inhibitor, selinexor, received approval as single agent for relapsed or refractory (R/R) DLBCL. Elucidating mechanisms by which overexpression supports cancer cells could facilitate further clinical development of inhibitors. We uncovered here that increases tolerance to genotoxic stress, leading a...
<div>Abstract<p>Exportin-1 (XPO1), the main soluble nuclear export receptor in eukaryotic cells, is frequently overexpressed diffuse large B-cell lymphoma (DLBCL). A selective XPO1 inhibitor, selinexor, received approval as single agent for relapsed or refractory (R/R) DLBCL. Elucidating mechanisms by which overexpression supports cancer cells could facilitate further clinical development of inhibitors. We uncovered here that increases tolerance to genotoxic stress, leading a...
<p>Supplementary Figures and Tables</p>