A5037509240- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Cannabis and Cannabinoid Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Prenatal Substance Exposure Effects
- Advanced Fluorescence Microscopy Techniques
- Reproductive System and Pregnancy
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Reproductive Health and Technologies
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Cardiovascular Effects of Exercise
- Reproductive biology and impacts on aquatic species
- Adenosine and Purinergic Signaling
- Effects and risks of endocrine disrupting chemicals
- Galectins and Cancer Biology
- Leptospirosis research and findings
- Extracellular vesicles in disease
- Diversity and Career in Medicine
- Neuroscience of respiration and sleep
- Ubiquitin and proteasome pathways
- Birth, Development, and Health
- Pluripotent Stem Cells Research
University of Massachusetts Amherst
2015-2024
University of Connecticut
2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2024
Universidad Maimónides
2024
University of Chicago
2024
Universidad de Los Andes, Chile
2024
Second Affiliated Hospital of Fujian Medical University
2024
Fujian Medical University
2024
Experimental Medicine and Biology Institute
2024
Northwestern University
2023
Sperm capacitation is required for fertilization. At the molecular level, this process associated with a fast activation of protein kinase A. Downstream event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity kinase(s) mediating has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested role PYK2 based on use small molecule inhibitors directed against kinase. However, critical loss-of-function reported. Here,...
Abstract Mammalian sperm are stored in the epididymis a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased demand during capacitation is well established, it remains unclear how mouse modify their metabolism meet need. We now show that capacitating enhance glucose uptake, identifying uptake as functional marker of Using an extracellular flux analyzer, we glycolysis oxidative...
Regulatory T cells (Tregs) modulate tissue homeostatic processes and immune responses. Understanding tissue-Treg biology will contribute to developing precision-targeting treatment strategies. Here, we show that Tregs maintain the tolerogenic state of testis epididymis, where sperm are produced mature. We found Treg depletion induces severe autoimmune orchitis epididymitis, manifested by an exacerbated cell infiltration [CD4 cells, monocytes, mononuclear phagocytes (MPs)] development...
Anandamide (AEA), a major endocannabinoid, binds to cannabinoid and vanilloid receptors (CB1, CB2 TRPV1) affects many reproductive functions. Nanomolar levels of anandamide are found in fluids including mid-cycle oviductal fluid. Previously, we that R(+)-methanandamide, an analogue, induces sperm releasing from bovine epithelium the CB1 antagonist, SR141716A, reversed this effect. Since detachment may be due surface remodeling brought about by capacitation, aim paper was investigate whether...
ABSTRACT Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility one features that allows them to succeed fertilization, and their flagellum essential for this function. Longitudinally, can be divided into midpiece, principal piece end piece. A precise cytoskeletal architecture tail key acquisition fertilization competence. It has been proposed actin cytoskeleton plays roles regulation motility; however, organization remains elusive. In...
Abstract To become fertile, mammalian sperm are required to undergo capacitation in the female tract or vitro defined media containing ions (e.g. HCO3 −, Ca2+, Na+, and Cl−), energy sources glucose, pyruvate) serum albumin bovine (BSA)). These different molecules initiate sequential concomitant signaling pathways, leading capacitation. Physiologically, induces changes motility pattern hyperactivation) prepares for acrosomal reaction (AR), two events fertilization. Molecularly, − activates...
Anandamide binds to cannabinoid receptors and plays several central peripheral functions. The aim of this work was study the possible role for endocannabinoid in controlling sperm-oviduct interaction mammals. We observed that bull sperm bovine oviductal epithelial cells express receptors, CB1 CB2, fatty acid amide hydrolase, enzyme controls intracellular anandamide levels. A quantitative assay determine whether involved developed. R(+)-methanandamide, a non-hydrolysable analog, inhibited...
Protein kinase A (PKA) is a broad-spectrum Ser/Thr involved in the regulation of several cellular activities. Thus, its precise activation relies on being localized at specific subcellular places known as discrete PKA signalosomes. A-Kinase anchoring proteins (AKAPs) form scaffolding assemblies that play pivotal role by restricting activity to microdomains. Because one first signaling events observed during mammalian sperm capacitation activation, understanding how restricted space and time...
To become fertile, mammalian sperm must undergo a series of biochemical and physiological changes known as capacitation. These involve crosstalk between metabolic signaling pathways can be recapitulated in vitro. In this work, were incubated the absence exogenous nutrients (starved) until they no longer able to move. Once immotile, energy substrates added back media motility was rescued. Following rescue, significantly higher percentage starved attained hyperactivated displayed increased...
Abstract Mammalian sperm acquire fertilization capacity in the female reproductive tract a process known as capacitation. During capacitation, change their motility pattern (i.e., hyperactivation) and become competent to undergo acrosome reaction. We have recently shown that, mouse, capacitation is associated with increased uptake of fluorescently labeled deoxyglucose extracellular acidification suggesting enhanced glycolysis. Consistently, present work we showed that glucose consumption...
Bioactive lipid molecules as lysophosphatidic acid (LPA), prostaglandins (PG) and endocannabinoids are important mediators of embryo implantation. Based on previous published data we became interested in studying the interaction between these three groups derivatives rat uterus during window Thus, adopted a pharmacological approach vitro using LPA, DGPP (a selective antagonist LPA3, an LPA receptor), endocannabinoids' receptor antagonists (AM251 AM630) non (indomethacin) (NS-398) inhibitors...
Mammalian spermatozoa are not able to fertilize an egg immediately upon ejaculation. They acquire this ability during their transit through the female genital tract in a process known as capacitation. The mammalian oviduct acts functional sperm reservoir providing suitable environment that allows maintenance of fertilization competence until ovulation occurs. After ovulation, gradually released from oviductal caudal isthmus and ascend site fertilization. Capacitating-related changes plasma...
ABSTRACT The CatSper cation channel is essential for sperm capacitation and male fertility. multi-subunit complexes form highly organized calcium signaling nanodomains on flagellar membranes. Here, we report identification of an uncharacterized protein, C2CD6, as a subunit the mouse complex. C2CD6 contains calcium-dependent, membrane-targeting C2 domain. associates with calcium-selective, core-forming subunits. Deficiency depletes from flagellum results in sterility. C2CD6-deficient are...
Is image-based flow cytometry a useful tool to study intracellular events in human sperm such as protein tyrosine phosphorylation or signaling processes?Image-based is powerful relevant number of cells, which enables robust statistical analysis providing spatial resolution terms the specific subcellular localization labeling.Sperm capacitation required for fertilization. During this process, spermatozoa undergo numerous physiological changes, via activation different pathways, are not...
To acquire fertilization competence, mammalian sperm must undergo several biochemical and physiological modifications known as capacitation. Despite its relevance, the metabolic pathways that regulate capacitation-related events, including development of hyperactivated motility, are still poorly described. Previous studies from our group have shown temporary energy restriction in mouse enhanced hyperactivation, vitro fertilization, early embryo pregnancy rates after transfer, it improved...
Abstract We have previously shown that members of the family testis‐specific serine/threonine kinases (TSSKs) are post‐meiotically expressed in testicular germ cells and mature sperm mammals. The restricted post‐meiotic expression TSSKs as well importance phosphorylation signaling processes strongly suggest an important role cell differentiation and/or function. This prediction has been supported by reported sterile phenotype TSSK6 knock‐out (KO) mice double TSSK1/TSSK2 KO. aim this study...
Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete the opportunity to fertilize oocyte. Whilst undergo capacitation essential fertilization. Sperm-oviduct interaction is sperm tightly regulated process influenced by local microenvironment. Previously we reported that endocannabinoid anandamide (AEA) regulates release from epithelial oviductal cells promoting capacitation. The aims of this work were measure AEA content characterize main...
Sperm capacitation has been largely associated with an increase in cAMP, although its relevance the underlying mechanisms of this maturation process remains elusive. Increasing evidence shows that extrusion cAMP through multidrug resistance protein 4 (MRP4) regulates cell homeostasis not only physiological but also pathophysiological situations and studies from our laboratory strongly support assumption. In present work we sought to establish role efflux regulation sperm capacitation. was...
Filamentous actin (F-actin) is a key factor in exocytosis many cell types. In mammalian sperm, acrosomal (denoted the acrosome reaction or AR), special type of controlled secretion, regulated by multiple signaling pathways and cytoskeleton. However, dynamic changes cytoskeleton live sperm are largely not understood. Here, we used powerful properties SiR-actin to examine dynamics mouse at onset AR. By using combination super-resolution microscopy techniques image loaded with from transgenic...
The oviduct acts as a functional sperm reservoir in many mammalian species. Both binding and release of spermatozoa from the oviductal epithelium are mainly modulated by capacitation. Several molecules fluid involved regulation function. Anandamide is lipid mediator reproductive physiology. Previously, we demonstrated that anandamide, through activation cannabinoid receptor type 1 (CB1), promotes bovine epithelial cells, CB1 transient potential vanilloid (TRPV1), induces Herein investigate...