A5059700682- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Biochemical and Molecular Research
- Genetic Syndromes and Imprinting
- Erythrocyte Function and Pathophysiology
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Polyamine Metabolism and Applications
- Neonatal Health and Biochemistry
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Sperm and Testicular Function
- Bacteriophages and microbial interactions
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Amino Acid Enzymes and Metabolism
University of Massachusetts Amherst
2016-2025
Amherst College
2011-2024
University of Pennsylvania
2005-2010
University of Passau
2008
Howard Hughes Medical Institute
2005
University of North Carolina at Chapel Hill
2001-2003
Pediatrics and Genetics
2003
Hebrew University of Jerusalem
1965-1980
Rabin Medical Center
1962-1970
Tel Aviv University
1965-1970
In vitro culture of mouse embryos results in loss imprinting. The aim the present study was to examine how two techniques commonly used during assisted reproduction, namely embryo and transfer, affect genomic imprinting after implantation mouse. F1 hybrid were subjected three experimental conditions: control (unmanipulated), transfer in-vitro-culture followed by transfer. Concepti collected on d9.5 development allelic expression determination ten imprinted genes (H19, Snrpn, Igf2, Kcnq1ot1,...
Egg activation, which is the first step in initiation of embryo development, involves both completion meiosis and progression into mitotic cycles. In mammals, fertilizing sperm delivers activating signal, consists oscillations free cytosolic Ca2+ concentration ([Ca2+]i). Intracytoplasmic injection (ICSI) a technique that vitro fertilization clinics use to treat myriad male factor infertility cases. Importantly, some patients who repeatedly fail ICSI also induce egg activation are, therefore,...
Uncontrolled inflammation is responsible for acute and chronic diseases in the lung. Regulating expression of pro-inflammatory genes pulmonary tissue using small interfering RNA (siRNA) a promising approach to combatting respiratory diseases. However, siRNA therapeutics are generally hindered at cellular level by endosomal entrapment delivered cargo organismal inefficient localization tissue. Here we report efficient anti-inflammatory activity vitro vivo polyplexes an engineered cationic...
SUMMARY: The turbidity of suspensions various Gram-negative bacteria is affected by the tonicity suspending medium. light extinction suspension increases in a nearly exponential fashion with solute concentration and approaches maximum at concentrations close to 0·15m-NaCl. maximal optical response changes varies widely different bacterial species, ranging from 30 140 % increase as compared distilled water. seem depend on viability cells they cannot be elicited killed procedures. influence...
Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here, we combine genome-wide mapping SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation human embryonic stem cells (hESCs). We find that DIGIT is divergent Goosecoid (GSC) and expressed differentiation. Deletion the SMAD3-occupied enhancer proximal inhibits GSC definitive Disruption gene encoding depletion transcript reveal...
Preconception environmental conditions have been demonstrated to shape sperm epigenetics and subsequently offspring health development. Our previous findings in humans showed that urinary anti-androgenic phthalate metabolites males were associated with altered methylation blastocyst-stage embryo To corroborate this, we examined the effect of preconception exposure di(2-ethylhexyl) (DEHP) on genome-wide DNA gene expression profiles mice. Eight-week old C57BL/6J male mice exposed either a...