A5011954284- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Reproductive biology and impacts on aquatic species
- Neuroendocrine regulation and behavior
- Ovarian function and disorders
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Orbital Angular Momentum in Optics
- Micro and Nano Robotics
- Plant Reproductive Biology
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- Cephalopods and Marine Biology
- Marine Invertebrate Physiology and Ecology
- Ion Channels and Receptors
- Circadian rhythm and melatonin
- Cellular transport and secretion
- Marine Biology and Environmental Chemistry
- Microfluidic and Bio-sensing Technologies
- Protein Structure and Dynamics
Universidad Nacional Autónoma de México
2015-2024
Yale University
1983-2024
Colorado State University
2024
Centro de Ingeniería Genética y Biotecnología
2024
Instituto de Biotecnología de León
1994-2024
Universidad Autónoma del Estado de Morelos
2007-2023
Autonomous University of San Luis Potosí
2023
Universidad Americana de Morelos
1998-2021
Instituto Gulbenkian de Ciência
2020
University of Massachusetts Amherst
2012
Here we show a unique example of male infertility conferred by gene knockout the sperm‐specific, pH‐dependent SLO3 potassium channel. In striking contrast to wild‐type sperm which undergo membrane hyperpolarization during capacitation, found that mutant depolarization. Several defects in are evident under capacitating conditions, including impaired motility, bent “hairpin” shape, and failure acrosome reaction (AR). The AR is rescued valinomycin hyperpolarizes sperm. Thus principal channel...
Fertilization occurs after the completion of sperm acrosome reaction, a secretory event that is triggered during gamete adhesion. ZP3, an egg zona pellucida glycoprotein, produces sustained increase internal Ca(2+) concentration in mouse sperm, leading to reactions. Here we show due persistent activation influx mechanism late stages ZP3 signal transduction. These cells also possess store depletion-activated entry pathway open treatment with thapsigargin. Thapsigargin and activate same...
Mammalian sperm are incapable of fertilizing eggs immediately after ejaculation; they acquire fertilization capacity residing in the female tract for a finite period time. The physiological changes undergo reproductive that render able to fertilize constitute phenomenon "sperm capacitation." We have demonstrated capacitation is associated with an increase tyrosine phosphorylation subset proteins and these events regulated by HCO(3)(-)/cAMP-dependent pathway involving protein kinase A....
Mammalian sperm acquire fertilizing ability in the female tract a process known as capacitation. At molecular level, capacitation is associated with up-regulation of cAMP-dependent pathway, changes intracellular pH, Ca(2+), and an increase tyrosine phosphorylation. How these signaling systems interact during not well understood. Results presented this study indicate that Ca(2+) ions have biphasic role regulation signaling. Media without added salts (nominal zero Ca(2+)) still contain...
Significance Sperm capacitation enables spermatozoa to undergo the acrosome reaction and exhibit vigorous motility called hyperactivation. At molecular level, is associated with activation of a cAMP-dependent pathway increase intracellular pH Ca 2+ concentrations. ionophore A23187 elevates induces but renders motionless. However, when was washed away, recovered motility, showed hyperactivation, were able fertilize cumulus-intact eggs. In these conditions, sperm acquired fertilizing capacity...
Sperm capacitation is required for fertilization and involves several ion permeability changes. Although Cl(-) HCO(3)(-) are essential capacitation, the molecular entities responsible their transport not fully known. During mouse sperm intracellular concentration of ([Cl(-)](i)) increases membrane potential (Em) hyperpolarizes. As in noncapacitated sperm, equilibrium appears to be close cell resting Em, opening channels could support [Cl(-)](i) increase observed during capacitation....
Sperm capacitation is required for fertilization. At the molecular level, this process associated with a fast activation of protein kinase A. Downstream event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity kinase(s) mediating has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested role PYK2 based on use small molecule inhibitors directed against kinase. However, critical loss-of-function reported. Here,...
Flagellar beating drives sperm through the female reproductive tract and is vital for reproduction. waves are generated by thousands of asymmetric molecular components; yet, paradoxically, forward swimming arises via symmetric side-to-side flagellar movement. This led to preponderance control hypotheses. However, asymmetries must still dictate flagellum be manifested in beat. Here, we reconcile microscopic observations, reconnecting structure function, showing that human uses anisotropic...
Abstract The resolution of fluorescence microscopy images is limited by the physical properties light. In last decade, numerous super-resolution (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on Mean Shift theory, which extends spatial single beyond diffraction limit MSSR works low and high fluorophore densities, not architecture optical setup applicable as well temporal series. theoretical...
Intracellular Ca(2+) has an important regulatory role in the control of sperm motility, capacitation, and acrosome reaction (AR). However, little is known about molecular identity membrane systems that regulate sperm. In this report, we provide evidence for expression seven Drosophila transient receptor potential homolog genes (trp1-7) three their protein products (Trp1, Trp3 Trp6) mouse Allegedly some trps encode capacitative channels. Immunoconfocal images showed while Trp6 was present...
There is pharmacological evidence that Ca2+ channels play an essential role in triggering the mammalian sperm acrosome reaction, exocytotic process required for to fertilize egg. Spermatozoa are small terminally differentiated cells difficult study by conventional electrophysiological techniques. To identify members of voltage-dependent channel family possibly present sperm, we have looked expression alpha 1A, 1B, 1C, 1D and 1E genes mouse testis purified spermatogenic cell populations with...
Ca2+ entry through channels is likely to play an important role in the differentiation of male germ cells as well fertilization by mature sperm. Here we present a detailed analysis currents expressed acutely dissociated mouse primary spermatocytes. Patch-clamp recordings demonstrated that only voltage-gated belong family T-type currents. Accordingly, Ni2+ (200 microM) and amiloride (500 reduced current amplitude 75 62%, respectively. To our knowledge, this first report system where are...
Eggs of many marine and mammalian species attract sperm by releasing chemoattractants that modify the bending properties flagella to redirect paths toward egg. This process, called chemotaxis, is dependent on extracellular Ca2+. We used stroboscopic fluorescence imaging measure intracellular Ca2+ concentration ([Ca2+]i) in swimming sea urchin sperm. Uncaging cyclic GMP induced entry via at least two distinct pathways, we identified a nimodipine-sensitive pathway, compartmentalized flagella,...