- Advanced Fluorescence Microscopy Techniques
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Seed Germination and Physiology
- Plant Reproductive Biology
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
- Quantum-Dot Cellular Automata
- Receptor Mechanisms and Signaling
- Click Chemistry and Applications
- Biomedical and Engineering Education
- Advanced Electron Microscopy Techniques and Applications
- Health and Medical Research Impacts
- Digital Imaging for Blood Diseases
- AI in cancer detection
- Genetics, Bioinformatics, and Biomedical Research
- Smart Agriculture and AI
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Global Health and Surgery
- Digital Holography and Microscopy
- Ovarian function and disorders
- Cell Image Analysis Techniques
Universidad Nacional Autónoma de México
2021-2024
Yale University
2024
Colorado State University
2024
Abstract The resolution of fluorescence microscopy images is limited by the physical properties light. In last decade, numerous super-resolution (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on Mean Shift theory, which extends spatial single beyond diffraction limit MSSR works low and high fluorophore densities, not architecture optical setup applicable as well temporal series. theoretical...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after acrosomal exocytosis (AE) interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a...
In September 2023, the two largest bioimaging networks in Americas, Latin America Bioimaging (LABI) and BioImaging North (BINA), came together during a 1-week meeting Mexico. This provided opportunities for participants to interact closely with decision-makers from imaging core facilities across Americas. The was held hybrid format attended in-person by scientists including Canada, United States, Mexico, Colombia, Peru, Argentina, Chile, Brazil Uruguay. aims of were discuss progress achieved...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after acrosomal exocytosis (AE) interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a...
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that extends spatial resolution in fluorescence images, beyond diffraction limit. MSSR works low- and high-density fluorophore not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single images as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after AE interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a decrease diameter driven...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after acrosomal exocytosis interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a decrease...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after AE interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a decrease diameter driven...
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that improves spatial resolution in fluorescence images beyond diffraction limit. MSSR works low- and high-density fluorophore images, not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...
Image-Based Flow Cytometry (IBFC) is a potent tool for the detailed analysis and quantification of cells in intricate samples, facilitating comprehensive understanding biological processes. This study leverages ResNet50 model to address IBFC's object-defocusing issue, an inherent challenge when imaging 3D object with stationary optics. A dataset 604 mouse sperm IBFC images (both bright field fluorescence) underpins exceptional capability reliably identify optimally focused head flagella...