A5034474404- Advanced Fluorescence Microscopy Techniques
- Neurobiology and Insect Physiology Research
- Digital Holography and Microscopy
- Phytochemicals and Antioxidant Activities
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Ion Channels and Receptors
- T-cell and B-cell Immunology
- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- CAR-T cell therapy research
- Photoacoustic and Ultrasonic Imaging
Universidad Nacional Autónoma de México
2019-2023
Abstract The resolution of fluorescence microscopy images is limited by the physical properties light. In last decade, numerous super-resolution (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on Mean Shift theory, which extends spatial single beyond diffraction limit MSSR works low and high fluorophore densities, not architecture optical setup applicable as well temporal series. theoretical...
Summary CD 43 is one of the most abundant co‐stimulatory molecules on a T‐cell surface; it transduces activation signals through its cytoplasmic domain, contributing to modulation outcome responses. The aim this study was uncover new signalling pathways regulated by sialomucin. Analysis changes in protein abundance allowed us identify pyruvate kinase isozyme M2 ( PKM 2), an enzyme glycolytic pathway, as element potentially participating cascade resulting from engagement and receptor TCR )....
Abstract Store-operated calcium entry (SOCE) is an essential influx mechanism in animal cells. One of the most important auto regulatory control systems involves calcium-dependent inactivation (CDI) Orai channel, which prevents excessive influx. In present study we analyze role two channels induction CDI on Orai1. Here show that entering through freely diffusing TRPV1 induce strong Orai1 while P2X 4 channel does not. can because both were found close proximity cell membrane. This was not...
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that extends spatial resolution in fluorescence images, beyond diffraction limit. MSSR works low- and high-density fluorophore not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single images as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...