A5021920937- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Reproductive Biology and Fertility
- Photoacoustic and Ultrasonic Imaging
- Sperm and Testicular Function
- Optical Coherence Tomography Applications
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Virus-based gene therapy research
- Biomedical and Engineering Education
- Computational Geometry and Mesh Generation
- Advanced Numerical Analysis Techniques
- Orbital Angular Momentum in Optics
- Functional Brain Connectivity Studies
- Primate Behavior and Ecology
- Diatoms and Algae Research
- Digital Holography and Microscopy
- Artificial Intelligence in Healthcare
- Advanced Materials and Mechanics
- Micro and Nano Robotics
- Ovarian function and disorders
- Animal Virus Infections Studies
- Bioinformatics and Genomic Networks
- Global Health and Surgery
Universidad Nacional Autónoma de México
2016-2024
Autonomous University of San Luis Potosí
2023
Abstract The resolution of fluorescence microscopy images is limited by the physical properties light. In last decade, numerous super-resolution (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on Mean Shift theory, which extends spatial single beyond diffraction limit MSSR works low and high fluorophore densities, not architecture optical setup applicable as well temporal series. theoretical...
Rotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins their organization VPs have lacked molecular-scale spatial resolution, due to inherent resolution constraints. In this work we employed super-resolution reveal nanometric-scale formed during infection, quantitatively describe structural seven viral within around...
The flagellar movement of the mammalian sperm plays a crucial role in fertilization. In female reproductive tract, human spermatozoa undergo process called capacitation which promotes changes their motility. Only capacitated may be hyperactivated and only those that transition to motility are capable fertilizing egg. Hyperactivated is characterized by asymmetric bends greater amplitude lower frequency. Historically, clinical fertilization studies have used two-dimensional analysis classify...
In September 2023, the two largest bioimaging networks in Americas, Latin America Bioimaging (LABI) and BioImaging North (BINA), came together during a 1-week meeting Mexico. This provided opportunities for participants to interact closely with decision-makers from imaging core facilities across Americas. The was held hybrid format attended in-person by scientists including Canada, United States, Mexico, Colombia, Peru, Argentina, Chile, Brazil Uruguay. aims of were discuss progress achieved...
Human spermatozoa must swim through the female reproductive tract, where they undergo a series of biochemical and biophysical reactions called capacitation, necessary step to fertilize egg. Capacitation promotes changes in motility pattern. Historically, two-dimensional analysis has been used classify sperm clinical fertilization studies. Nevertheless, natural environment is three-dimensional (3D). Imaging flagella freely swimming difficult task due their high beating frequency up 25 Hz....
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that extends spatial resolution in fluorescence images, beyond diffraction limit. MSSR works low- and high-density fluorophore not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single images as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...
Abstract In the realm of 3D image processing, accurately representing geometric nuances line curves is crucial. Building upon foundation set by slope chain code, which adeptly represents intricate two-dimensional using an array capturing exterior angles at each vertex, this study introduces innovative encoding method tailored for polygonal curves. This employs parallel and torsion chains, ensuring invariance to common transformations like translations, rotations, uniform scaling, while also...
The flagellar movement of the mammalian sperm is essential for male fertility as it enables this cell to reach and fertilize an egg. In female reproductive tract, human spermatozoa undergo a process called capacitation which promotes changes in their motility. Only those that change hyperactivated (HA) motility are capable fertilizing egg; type characterized by asymmetric bends greater amplitude lower frequency. Historically, clinical fertilization studies have used two-dimensional analysis...
This article describes a computer system to support decisions in Intensive Medicine developed for multipurpose Care Unit (13 beds and about 700 yearly patients). The is used microcomputer (486DX4). Works done this area are mentioned; the different programs which integrate system, advantages of its use results accomplished described. Some most important quality increment medical care given, physicians' possibilities dedicating more time their patients, cost reductions, improvements...
Abstract Summary FCSlib is an open-source R tool for fluorescence fluctuation spectroscopy data analysis. It encompasses techniques such as Fluorescence Correlation Spectroscopy, Number and Brightness, Pair Function of Molecular Brightness. Availability implementation Source code available at https://cran.r-project.org/web/packages/FCSlib/ Linux, Windows macOS platforms. Sample well a user's guide are https://github.com/FCSlib/FCSlib. Supplementary information Bioinformatics online.
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that improves spatial resolution in fluorescence images beyond diffraction limit. MSSR works low- and high-density fluorophore images, not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...
Abstract The flagellar movement of the mammalian sperm is essential for male fertility as it enables this cell to reach and fertilize an egg. In female reproductive tract, human spermatozoa undergo a process called capacitation which promotes changes in their motility. Only those that change hyperactivated (HA) motility are capable fertilizing egg; type characterized by asymmetric bends greater amplitude lower frequency. Historically, clinical fertilization studies have used two-dimensional...
Abstract In the realm of 3D image processing, accurately representing geometric nuances line curves is crucial. Building upon foundation set by Slope Chain Code (SCC), which adeptly represents intricate two-dimensional using an array capturing exterior angles at each vertex, this study introduces innovative encoding method tailored for polygonal curves. This employs parallel slope and torsion chains, ensuring invariance to common transformations like translations, rotations, uniform scaling,...
Abstract Rotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viro-plasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins their organization VPs have lacked molecular-scale spatial resolution, due to inherent resolution constraints. In this work we employed super-resolution reveal nanometric-scale formed during infection, quantitatively describe structural seven viral dsRNA...
ABSTRACT Due to the wave nature of light, optical microscopy has a lower-bound lateral resolution limit about half wavelength detected i.e., within range 200 300 nm. The Fluorescence Fluctuation based Super Resolution Microscopy (FF-SRM) encompases collection image analysis techniques which rely on statistical processing temporal variations fluorescence reduce uncertainty fluorophore positions sample, hence, bringing spatial down several tens FF-SRM is known be suitable for live-cell imaging...