A5084430640- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Sperm and Testicular Function
- Cell Image Analysis Techniques
- Plant Reproductive Biology
- Seed Germination and Physiology
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Functional Brain Connectivity Studies
- Erythrocyte Function and Pathophysiology
- Chronic Lymphocytic Leukemia Research
- Single-cell and spatial transcriptomics
- Advanced Electron Microscopy Techniques and Applications
- Optical Coherence Tomography Applications
- Photosynthetic Processes and Mechanisms
- Biocrusts and Microbial Ecology
- Hydrological Forecasting Using AI
- Plant Stress Responses and Tolerance
- Electrochemical Analysis and Applications
- Hydrology and Watershed Management Studies
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Bioinformatics and Genomic Networks
- Electrochemical sensors and biosensors
- Ovarian function and disorders
Universidad Nacional Autónoma de México
2021-2024
Yale University
2024
Colorado State University
2024
European Molecular Biology Laboratory
2023
Marine Biological Laboratory
2022
Tecnologie Avanzate (Italy)
2020
Abstract The resolution of fluorescence microscopy images is limited by the physical properties light. In last decade, numerous super-resolution (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on Mean Shift theory, which extends spatial single beyond diffraction limit MSSR works low and high fluorophore densities, not architecture optical setup applicable as well temporal series. theoretical...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after acrosomal exocytosis (AE) interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a...
Abstract During seed maturation, plants may experience severe desiccation, leading to the accumulation of late embryogenesis abundant (LEA) proteins. These intrinsically disordered proteins also accumulate in plant tissues under water deficit. Functional roles LEA have been proposed based on vitro studies, where monomers are considered as functional units. However, potential formation homo‐oligomers has little explored. In this work, we investigated self‐association Arabidopsis thaliana...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after acrosomal exocytosis (AE) interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a...
Spatial biology is a rapidly growing research field that focuses on the transcriptomic or proteomic profiling of single cells within tissues with preserved spatial information. Imaging-based transcriptomics uses epifluorescence microscopy, which has shown remarkable results for identification multiple targets in situ. Nonetheless, number genes can be reliably visualized limited by diffraction light. Here, we investigate effect structured illumination (SIM), super-resolution microscopy...
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that extends spatial resolution in fluorescence images, beyond diffraction limit. MSSR works low- and high-density fluorophore not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single images as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...
Spatial biology is a rapidly growing research field which focuses on the transcriptomic or proteomic profiling of single cells within tissues with preserved spatial information. Imaging-based transcriptomics uses epifluorescence microscopy, has shown remarkable results for identification multiple targets in situ. Nonetheless, number genes that can be reliably visualized limited by diffraction light. Here, we investigate effect structured illumination (SIM), super-resolution microscopy...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after AE interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a decrease diameter driven...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after acrosomal exocytosis interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a decrease...
Mammalian sperm delve into the female reproductive tract to fertilize gamete. The available information about how regulate their motility during final journey fertilization site is extremely limited. In this work, we investigated structural and functional changes in flagellum after AE interaction with eggs. evidence demonstrates that double helix actin network surrounding mitochondrial sheath of midpiece undergoes prior cessation. This modification accompanied by a decrease diameter driven...
Abstract Summary FCSlib is an open-source R tool for fluorescence fluctuation spectroscopy data analysis. It encompasses techniques such as Fluorescence Correlation Spectroscopy, Number and Brightness, Pair Function of Molecular Brightness. Availability implementation Source code available at https://cran.r-project.org/web/packages/FCSlib/ Linux, Windows macOS platforms. Sample well a user's guide are https://github.com/FCSlib/FCSlib. Supplementary information Bioinformatics online.
Abstract Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that improves spatial resolution in fluorescence images beyond diffraction limit. MSSR works low- and high-density fluorophore images, not limited by architecture of detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) applicable to single as well temporal series. The theoretical limit resolution, optimized real-world imaging conditions analysis image series, has been measured be 40...
ABSTRACT Due to the wave nature of light, optical microscopy has a lower-bound lateral resolution limit about half wavelength detected i.e., within range 200 300 nm. The Fluorescence Fluctuation based Super Resolution Microscopy (FF-SRM) encompases collection image analysis techniques which rely on statistical processing temporal variations fluorescence reduce uncertainty fluorophore positions sample, hence, bringing spatial down several tens FF-SRM is known be suitable for live-cell imaging...